Reagent and detection for detecting porcine senecavirus A and application of reagent

Abstract

The invention discloses a reagent and a detection method for detecting porcine senecavirus A and an application of the reagent. The reagent for detecting the porcine senecavirus A provided by the invention comprises a primer pair and a probe, wherein in the primer pair, the sequence an upstream primer is shown as SEQ ID No.1 and the sequence a downstream primer is shown as SEQ ID No.2; the sequence of the probe is shown as SEQ ID No.3 or a reverse complementary sequence of the sequence; in the probe sequence shown as SEQ ID No.3, a 6-fluorophore-dT is modified by the 31st base group, the 32ndbase group is substituted into a base analogue; a fluorescent quencher-dT is modified by the 33rd base group; and C3 Spacer is modified by 3' terminal. The reagent provided by the invention, through recombinase polymerase amplification, can conduct sensitive, specific and efficient detection on the SVA (the senecavirus A). In comparison with existing detection methods, the reagent and the method provided by the invention are short in time and easy to operate, and a conclusion can be reached rapidly; the reagent and the method are applicable to on-site detection; and the reagent and the methodare significant for the rapid prevention and control of the SVA.

Description

technical field [0001] The application relates to the field of detection of porcine type A Seneca virus, in particular to a reagent for detection of porcine type A Seneca virus and its application, and a detection method for porcine type A Seneca virus based on the reagent. Background technique [0002] Senecavirus A (SVA), formerly known as Seneca Valleyvirus (SVV), is the only member of the Picornaviridae family, genus Senecavirus. SVA is an icosahedral non-enveloped single-stranded positive-sense RNA virus with a diameter of about 27 nm and a genome length of about 7.2 kb. SVA was initially thought to be a contaminant in cell culture and was presumed to be derived from porcine pancreatic enzymes or fetal bovine serum. Before 2007, SVA was mainly studied as an oncolytic cell for the treatment of certain neuroendocrine tumors and has been considered a non-pathogenic virus. In 2007, a batch of pig nose mirrors shipped from Canada to Minnesota, USA showed symptoms of bliste...

AI Technical Summary

Problems solved by technology

[0003] Because the disease is a newly discovered zoonotic disease, the clinical symptoms of infected pigs are mainly manifested as vesicular lesions such as blisters and festering wounds on the nose and crown of the hooves, which is similar to a type of zoonotic disease in my country, foot-and-mouth disease (FMD). , Swine vesicular disease (SVD), if the accurate diagnosis is not made in time, it will lead to improper prevention and control measures, which will cause serious consequences

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