Method for determining uronic acid content in stigma maydis polysaccharide, and antioxidation action thereof
A technology of corn silk polysaccharide and uronic acid, which is applied in the field of analytical chemistry, can solve the problems of measurement error, uronic acid measured value deviates from the actual content, etc., and achieve high accuracy, small measurement error and good reproducibility
Inactive Publication Date: 2018-05-01
JILIN INST OF CHEM TECH
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[0004] In the sulfuric acid carbazole colorimetric method, since there are generally neutral monosaccharides, pentoses and hexoses in the solution to be tested, the absorption wavelength of uronic acid is the same as that of uronic acid, which will cause the measured value of uronic acid to deviate from the actual content. result in large errors
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[0019] The present invention will be described in detail below in combination with diagrams and specific implementation operations.
[0020] Accurately weigh 4 mg of galacturonic acid reference substance dried to constant weight at 70°C and place it in a 50 mL volumetric flask, dissolve it in distilled water to make up 80 mu g / mL of the reference substance solution.
[0021] Accurately weigh 0.0500g of corn silk polysaccharide under the same conditions, place it in a 50mL volumetric flask, dilute to the mark with distilled water, shake well, and centrifuge (10 min, 3000r / min) to prepare a 1mg / mL polysaccharide solution; The method of preparing 1 mg / mL fungus polysaccharide solution.
[0022] Weigh 0.477 g of borax and add 100 mL of concentrated sulfuric acid to dissolve it ultrasonically, and set aside.
[0023] Weigh 0.15 g of m-hydroxybiphenyl, dissolve it with 0.5% sodium hydroxide solution and dilute it to a 100 mL volumetric flask.
[0024] Precisely pipette 0.5 mL of...
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The invention discloses a method for determining a uronic acid content in stigma maydis polysaccharide based on an m-hydroxydiphenyl method. The method comprises the following steps of precisely weighing 0.0500g of stigma maydis polysaccharide into a 50-mL volumetric flask, dissolving with distilled water to dilute to a scale; shaking up, and preparing into 1mg / mL of polysaccharide solution; weighing 0.477g of borax, adding 100mL of concentrated sulfuric acid, and carrying out ultrasonic dissolving; weighing 0.15g of m-hydroxy diphenyl, dissolving with 0.5 percent of sodium hydroxide solution,and sizing into a 100-mL volumetric flask; precisely transferring 0.5mL of 1mg / mL of stigma maydis polysaccharide solution into a 10-mL volumetric flask, slowly adding 5mL of sulfuric acid-borax solution, shaking up, boiling in a boiling water bath for 5min, cooling to room temperature, adding 8muL of 0.15 percent of m-hydroxy diphenyl solution, uniformly mixing, and standing for 30min; using distilled water as a blank solution for zero setting, and using an ultraviolet spectrophotometer for determining an absorption value at a 528nm part; adopting a galacturonic acid solution as a standard liquid, drawing a standard curve, and substituting the absorption value of the polysaccharide into the standard curve for calculating the uronic acid content.
Description
technical field [0001] The invention relates to a method for determining the content of uronic acid in polysaccharides, in particular to a method for determining the content of uronic acid in polysaccharides based on the m-hydroxybiphenyl method, and belongs to the technical field of analytical chemistry. Background technique [0002] Uronic acid, also known as hyaluronic acid, is an acidic mucopolysaccharide. It is the most moisturizing substance found in nature. It is known as an ideal natural moisturizing factor. It is widely distributed in various parts of the human body, and the skin also contains A large amount of uronic acid, the maturation and aging process of human skin also changes with the content and metabolism of uronic acid, it can improve skin nutrition metabolism, make skin soft, smooth, wrinkle-free, increase elasticity, prevent aging, and in moisturizing It is also a good transdermal absorption accelerator. [0003] There are two methods for the determinat...
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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 周鸿立李亚平赵鹤鹏
Owner JILIN INST OF CHEM TECH