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Preparation method of LDI-PCLLA-PEG-PCLLA/hydroxyapatite electrospun fiber membrane as well as product and application thereof

A technology of hydroxyapatite and electrospun fibers, applied in medical science, prosthesis, tissue regeneration, etc., to achieve good biocompatibility, good mechanical properties, and good adhesion and crawling effects

Inactive Publication Date: 2018-05-04
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, single-component electrospun fiber membranes have been reported, but the blending and modification of PCLA1000-PEG2000-PCLA1000 and compounding Hap particles to prepare composite fiber membranes by electrospinning have not been reported yet.

Method used

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  • Preparation method of LDI-PCLLA-PEG-PCLLA/hydroxyapatite electrospun fiber membrane as well as product and application thereof
  • Preparation method of LDI-PCLLA-PEG-PCLLA/hydroxyapatite electrospun fiber membrane as well as product and application thereof
  • Preparation method of LDI-PCLLA-PEG-PCLLA/hydroxyapatite electrospun fiber membrane as well as product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Heat the PCLLA-PEG-PCLLA raw material to 50 degrees Celsius and vacuumize it. After filling the flask with inert and dry argon, add LDI to react and use magnetic stirring to keep the reaction temperature at 60 degrees Celsius overnight for about 48 hours. Mix 0.2 g of the obtained cross-linked substance IPDI-PEG-PCLLA with 2 g of hexafluoroisopropanol at a mass ratio of 0.1:1, sonicate for 30 minutes, and magnetically stir overnight to obtain a uniform solution. After stirring at room temperature for 6~12h, a homogeneous solution was obtained. The obtained solution was put into a 10 mL glass syringe, and the composite was obtained by electrospinning equipment under the condition of spinning voltage of 10-13 KV, spinning receiving distance of 10-15 cm, and solution flow rate of 0.32-0.4 mL / h. Nanofiber membrane support, after spinning, put the obtained membrane into a vacuum oven to dry overnight at room temperature for future use.

[0042] Among them, the SEM photos of...

Embodiment 2

[0046] Heat the PCLLA-PEG-PCLLA raw material to 50 degrees Celsius and vacuumize it. After filling the flask with inert and dry argon, add LDI to react and use magnetic stirring to keep the reaction temperature at 60 degrees Celsius overnight for about 48 hours. Mix 0.2 g of the obtained cross-linked substance LDI-PEG-PCLLA with 2 g of hexafluoroisopropanol at a mass ratio of 0.1:1, sonicate for 30 minutes, and add an additional 5% (LDI-PEG-PCLLA: Hap mass ratio) Hap 0.11 g, continue to sonicate for 30 minutes, and obtain a homogeneous solution after magnetic stirring overnight. After stirring at room temperature for 6~12h, a homogeneous solution was obtained. The obtained solution was put into a 20mL glass syringe, and composite nanofibers were obtained by electrospinning equipment under the conditions of spinning voltage 10-15 KV, tray receiving distance 12-15 cm, and push flow rate 0.32-0.40mL / h Membrane support, after the completion, put the obtained membrane into a vacuu...

Embodiment 3

[0048] Heat the PCLLA-PEG-PCLLA raw material to 50 degrees Celsius and vacuumize it. After filling the flask with inert and dry argon, add LDI to react and use magnetic stirring to keep the reaction temperature at 60 degrees Celsius overnight for about 48 hours. Mix 0.2 g of the obtained cross-linked substance LDI-PEG-PCLLA with 2 g of hexafluoroisopropanol at a mass ratio of 0.1:1, sonicate for 30 minutes, and add an additional 10% (LDI-PEG-PCLLA:Hap mass ratio) Hap 0.22g, continue to sonicate for 30 minutes, and obtain a homogeneous solution after magnetic stirring overnight. After stirring at room temperature for 6~12h, a homogeneous solution was obtained. The resulting solution was put into a 20mL glass syringe, and the composite nanofibers were obtained by electrospinning equipment under the conditions of spinning voltage 10-15 KV, tray receiving distance 12-15 cm, and push flow rate 0.32-0.40mL / h Membrane support, after the completion, put the obtained membrane into a v...

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Abstract

The invention relates to a method in the technical field of medical materials and in particular relates to a preparation method of an LDI-PCLLA-PEG-PCLLA / hydroxyapatite electrospun fiber membrane as well as a product and application thereof. The preparation method comprises the following steps: taking a main component of a body cross-linked structure biological scaffold which takes polycaprolactone polylactic acid-polyethylene glycol-polylactic acid (PCLLA-PEG-PCLLA) as a soft segment and takes L-lysine diisocyanate (LDI) as a hard segment, taking nano-sized hydroxyapatite particles as an inorganic filling material, and performing electrostatic spinning, thereby obtaining the composite nano fiber membrane. The compounded fiber membrane scaffold achieves effects of promoting bone growth andinducing osteoblast differentiation of cells.

Description

technical field [0001] The invention relates to a method in the technical field of medical materials, in particular to a method for preparing an LDI-PCLLA-PEG-PCLLA / hydroxyapatite electrospun fiber membrane and its product and application. The solid part is made of polycaprolactone polylactic acid-polyethylene glycol-polylactic acid (PCLLA-PEG-PCLLA) as the soft segment structure, and L-lysine diisocyanate (LDI) as the hard segment structure. The main component of the scaffold. Nanoscale hydroxyapatite particles are used as filler inorganic materials, and the composite nanofibrous membrane is prepared by electrospinning. The composite fibrous membrane scaffold can promote bone growth and induce cells to differentiate into osteoblasts. technical background [0002] The repair of tissue and organ defects has always been a major issue affecting human health. For a long time, scientists have been seeking various methods, means and materials for the treatment of tissue defects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/56A61L27/50A61L27/18C08G18/42
CPCA61L27/12A61L27/18A61L27/50A61L27/56A61L2430/02C08G18/4277C08L75/06
Inventor 何丹农严一楠刘训伟杨迪诚李士浩金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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