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Preparation method of high-efficiency medicament carrier material based on enzyme-degrading bacterium micro-environmental response

A carrier material and micro-environment technology, applied in prosthesis, medical science, coating, etc., can solve the problems of long-term infection, drug resistance, re-infection, etc., and achieve the effect of wide application range, good repeatability, and convenient operation

Inactive Publication Date: 2018-05-04
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, long-term use of antibiotics is likely to cause drug resistance, and some bacteria remain in the cells after being phagocytized by macrophages, resulting in re-infection or long-term infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Prepare a 0.5mg / mL MMT solution and adjust the pH to 7.0. Take 50ml of ultrapure water in a 50ml centrifuge tube, adjust the pH to 7.0, and mark it as washing solution 1.

[0020] Prepare 0.5 mg / mL gentamicin sulfate and dissolve it in 50 ml of 0.5 mg / ml PLL with magnetic stirring, and adjust the pH value to 9.5. Take 50ml of ultrapure water and adjust the pH value to 9.5 in a 50ml centrifuge tube, and mark it as washing solution 2.

[0021] Add the pretreated substrate into the MMT solution and soak for 6-8 minutes, take it out, put it in lotion 1 and wash it for 2-3 minutes, take it out, and use N 2 blow dry. Then put it into the PLL-GS solution and soak it for 6-8min, take it out, put it into the lotion 2 and wash it for 2-3min, take it out, and wash it with N 2 blow dry. So far, the preparation of a double layer is completed. 6, 8, 10 bilayers were prepared respectively.

[0022] Ellipsometric spectroscopy found that the thickness of the film layer increased l...

Embodiment 2

[0024] Prepare a 1mg / mL MMT solution and adjust the pH to 7.0. Take 50ml of ultrapure water in a 50ml centrifuge tube, adjust the pH to 7.0, and mark it as washing solution 1.

[0025] Prepare 0.5 mg / mL gentamicin sulfate and dissolve it in 50 ml of 0.5 mg / ml PLL with magnetic stirring, and adjust the pH value to 5.5. Take 50ml of ultrapure water and adjust the pH value to 5.5 in a 50ml centrifuge tube, and mark it as washing solution 2.

[0026] Add the pretreated substrate into the MMT solution and soak for 6-8 minutes, take it out, put it in lotion 1 and wash it for 2-3 minutes, take it out, and use N 2 blow dry. Then put it into the PLL-GS solution and soak it for 6-8min, take it out, put it into the lotion 2 and wash it for 2-3min, take it out, and wash it with N 2 blow dry. So far, the preparation of a double layer is completed. 6, 8, 10 bilayers were prepared respectively.

[0027] The antibacterial zone experiment found that the drug carrier has high antibacteria...

Embodiment 3

[0029] Prepare a 4mg / mL MMT solution and adjust the pH to 5.5. Take 50ml of ultrapure water in a 50ml centrifuge tube, adjust the pH to 5.5, and mark it as washing solution 1.

[0030] Dissolve 1 mg / mL gentamicin sulfate in 50 ml 2 mg / ml PLL with magnetic stirring, and adjust the pH to 5.5. Take 50ml of ultrapure water and adjust the pH value to 5.5 in a 50ml centrifuge tube, and mark it as washing solution 2.

[0031] Add the pretreated substrate into the MMT solution and soak for 6-8 minutes, take it out, put it in lotion 1 and wash it for 2-3 minutes, take it out, and use N 2 blow dry. Then put it into the PLL-GS solution and soak it for 6-8min, take it out, put it into the lotion 2 and wash it for 2-3min, take it out, and wash it with N 2 blow dry. So far, the preparation of a double layer is completed. 6, 8, 10 bilayers were prepared respectively.

[0032] Scanning electron microscope results showed that the prepared drug-loaded organic-inorganic hybrid coating had ...

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PUM

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Abstract

The invention discloses a preparation method of a high-efficiency medicament carrier material based on enzyme-degrading bacterium micro-environmental response. According to the preparation method of the high-efficiency medicament carrier material based on the enzyme-degrading bacterium micro-environmental response, small molecular gentamycin is loaded in a membrane, and the membrane is degraded under the stimulation of bacteria or chymotrypsin to release a medicament so as to realize a high-efficiency and long-term sterilizing function on gram-negative bacteria, gram-positive bacteria as wellas fungi. A medicament carrier has an enzyme and bacterial injection response medicament release effect, can realize controllability of medicament release, and avoids medicament resistance. The medicament carrier can realize broad-spectrum antibacterial capacity and controllable medicament release capacity; polylysine with good biocompatibility is added, so that the cellular compatibility of the medicament carrier is improved, and the medicament carrier shows low cytotoxicity on the lens epithelial cells and desmocytes. The preparation method is simple, has good repeatability and wide application range, and can perform coating modification on a biomedical device with a complex haplotype structure.

Description

technical field [0001] The invention specifically relates to the technical field of new materials, in particular to a method for preparing a high-efficiency drug carrier material based on enzymatic degradation of bacterial microenvironmental responses. Background technique [0002] Bacterial infection is currently a key factor limiting the clinical application of biomaterials. According to relevant studies, more than 16% of patients die of infection every year. After pathogenic microorganisms adhere to the surface of biological materials, a dense biofilm is formed on the surface of biological materials with the proliferation of pathogenic microorganisms, which seriously hinders the function of antibacterial media. Moreover, long-term use of antibiotics is likely to cause drug resistance, and some bacteria remain in the cells after being phagocytized by macrophages, resulting in reinfection or long-term infection. Therefore, realizing the controllability of drug release and ...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L27/40A61L31/10A61L31/08A61L31/14A61L31/16A61L27/34A61L27/30A61L27/56A61L27/58
CPCA61L31/10A61L27/306A61L27/34A61L27/56A61L27/58A61L31/088A61L31/148A61L31/16A61L2300/232A61L2300/404A61L2300/604A61L2420/04A61L2420/08C08L77/04
Inventor 王佰亮徐青文李溪金滢滢陈浩南开辉
Owner WENZHOU MEDICAL UNIV
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