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COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Turpan black sheep meat quality trait

A detection kit and a technology for meat quality traits, which are applied in the identification field of animal genetic selection and breeding, can solve problems such as no influence on meat quality, and achieve the effect of strong lignification and more crude fiber

Inactive Publication Date: 2018-05-04
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on sheep is mainly aimed at the research of the disease prevention and control process. There is no research on the effect of this gene on the meat quality of Turpan black sheep, and the specific research based on the improvement of the meat quality of the typical breed of Turpan black sheep
In the prior art, there is no research and report on the use of COL1A1 gene to improve the meat quality traits of Turpan black mutton at home and abroad.

Method used

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  • COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Turpan black sheep meat quality trait
  • COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Turpan black sheep meat quality trait

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A detection kit containing COL1A1 gene to improve the meat quality traits of Turpan black mutton, the kit was obtained through the construction of the following co-construction system:

[0033] (1) Design primer pairs: Use the mRNA sequence of the sheep COL1A1 gene to manually design specific primers. The primers are as follows:

[0034] COL1A1 primers:

[0035] Upstream primer F: 5'-TACAGCGTCACCTACGACGG-3';

[0036] Downstream primer R: 5'-AAGCCGAATTCCTGGTCTG-3'.

[0037] (2) PCR reaction reagents: Mix, upstream and downstream primers, cDNA template and RNase-free water.

Embodiment 2

[0039] The present invention provides the application of the detection kit containing COL1A1 gene for improving Turpan black mutton meat quality, comprising the following steps:

[0040] (1) Genomic RNA extraction:

[0041] 30-50 mg of longissimus dorsi muscle of Turpan black sheep to be tested was collected and stored in liquid nitrogen; RNA was extracted and stored at -80°C; reverse transcribed into cDNA, and PCR amplification was performed with the internal reference gene ACTB.

[0042] Preparation of 1.5% agarose gel: 1.5g of agarose, dissolved in 100ml of 0.5×TBE, heated in a microwave oven until fully dissolved, poured into the glue tank with a comb inserted after cooling to room temperature, and set aside after cooling and solidification.

[0043] The prepared 1.5% agarose gel was used for electrophoresis detection, the voltage was 100V, the current was 50mA, and the electrophoresis was carried out for 35 minutes. After dyeing with hexamethylene bromide for 15 minutes,...

Embodiment 3

[0057] Based on the second embodiment, the detection kit containing the COL1A1 gene provided in the above-mentioned embodiment to improve the meat quality traits of Turpan black mutton was applied to pigs, Ba Yinbrook sheep, Tashkurgan sheep, Bashibai sheep, Xinjiang fine wool sheep, and Kirgiz sheep, respectively. The relationship between the expression of COL1A1 gene in the longissimus dorsi and intramuscular fatty acid content, water content and shearing force of the longissimus dorsi was observed. See Table 2 for details:

[0058] Table 2: Association analysis of COL1A1 gene expression and some meat quality indicators

[0059]

fatty acid content

moisture content

Longissimus dorsi shear force

Turpan black sheep

0.883

0.995

1.00

pig

0.127

0.118

0.067

Octave Brooke Sheep

0.132

0.045

0.136

Tashkurgan sheep

0.075

0.141

0.151

bashbai sheep

0.126

0.166

0.171

Xinjiang fine w...

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Abstract

The invention discloses a COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Turpan black sheep meat quality trait. A specific primer is self and artificially designed according to an mRNA sequence of a sheep COL1A1 gene, an upstream primer is 5'-TACAGCGTCACCTACGACGG-3', a downstream primer is 5'-AAGCCGAATTCCTGGTCTG-3', sample genome DNA is utilized as a template to perform PCR proliferation, fluorescence quantitative PCR detection is performed on a PCR proliferation product, correlation analysis between gene expression quantity and intramuscular fatty acid content, moisture content and longissimus dorsi shear force is performed, and the COL1A1 gene-containing detection kit disclosed by the invention is shown to have better specificity to the Turpan black sheep. The COL1A1 gene-containing detection kit disclosed by the invention not only fills the blank of the research field at home and abroad, but also has important significance and actual application value inprotecting Turpan black sheep good stock resource development, establishing molecular theory basis for sustainable utilization and quickening Sinkiang mutton sheep industry development.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection and breeding, in particular, the invention relates to the technical field of a kit for selection and breeding of Turpan black mutton meat quality traits. Background technique [0002] Xinjiang is one of the five major pastoral areas in my country, and the total output of mutton ranks among the top in the country. At the same time, Xinjiang is a minority area, and the demand for mutton is increasing rigidly, making it difficult for the production of mutton in the region to meet the growing demand of people. To solve this problem, it is particularly important to analyze the genetic mechanism attached to the abundant and unique sheep breeds in Xinjiang. Turpan black sheep, also known as Toksun big-tailed black sheep, is formed by crossbreeding with short-fat-tailed Mongolian line (Bayinbulak big-tailed sheep) as male parent and fat-rumped Kazakh sheep as female parent after lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/158C12Q2600/166
Inventor 刘武军赵国艺方超马海玉方丽君刘玲玲于茜褚洪忠
Owner XINJIANG AGRI UNIV
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