COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Turpan black sheep meat quality trait
A detection kit and a technology for meat quality traits, which are applied in the identification field of animal genetic selection and breeding, can solve problems such as no influence on meat quality, and achieve the effect of strong lignification and more crude fiber
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Embodiment 1
[0032] A detection kit containing COL1A1 gene to improve the meat quality traits of Turpan black mutton, the kit was obtained through the construction of the following co-construction system:
[0033] (1) Design primer pairs: Use the mRNA sequence of the sheep COL1A1 gene to manually design specific primers. The primers are as follows:
[0034] COL1A1 primers:
[0035] Upstream primer F: 5'-TACAGCGTCACCTACGACGG-3';
[0036] Downstream primer R: 5'-AAGCCGAATTCCTGGTCTG-3'.
[0037] (2) PCR reaction reagents: Mix, upstream and downstream primers, cDNA template and RNase-free water.
Embodiment 2
[0039] The present invention provides the application of the detection kit containing COL1A1 gene for improving Turpan black mutton meat quality, comprising the following steps:
[0040] (1) Genomic RNA extraction:
[0041] 30-50 mg of longissimus dorsi muscle of Turpan black sheep to be tested was collected and stored in liquid nitrogen; RNA was extracted and stored at -80°C; reverse transcribed into cDNA, and PCR amplification was performed with the internal reference gene ACTB.
[0042] Preparation of 1.5% agarose gel: 1.5g of agarose, dissolved in 100ml of 0.5×TBE, heated in a microwave oven until fully dissolved, poured into the glue tank with a comb inserted after cooling to room temperature, and set aside after cooling and solidification.
[0043] The prepared 1.5% agarose gel was used for electrophoresis detection, the voltage was 100V, the current was 50mA, and the electrophoresis was carried out for 35 minutes. After dyeing with hexamethylene bromide for 15 minutes,...
Embodiment 3
[0057] Based on the second embodiment, the detection kit containing the COL1A1 gene provided in the above-mentioned embodiment to improve the meat quality traits of Turpan black mutton was applied to pigs, Ba Yinbrook sheep, Tashkurgan sheep, Bashibai sheep, Xinjiang fine wool sheep, and Kirgiz sheep, respectively. The relationship between the expression of COL1A1 gene in the longissimus dorsi and intramuscular fatty acid content, water content and shearing force of the longissimus dorsi was observed. See Table 2 for details:
[0058] Table 2: Association analysis of COL1A1 gene expression and some meat quality indicators
[0059]
fatty acid content
moisture content
Longissimus dorsi shear force
Turpan black sheep
0.883
0.995
1.00
pig
0.127
0.118
0.067
Octave Brooke Sheep
0.132
0.045
0.136
Tashkurgan sheep
0.075
0.141
0.151
bashbai sheep
0.126
0.166
0.171
Xinjiang fine w...
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