Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting food safety by chemiluminescence and preparation method of kit

A chemiluminescence detection and food safety technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of low sensitivity, high detection cost, easy inactivation, etc. The effect of good stability, low background luminescence and few interference factors

Inactive Publication Date: 2018-05-04
李梅秀
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbiological method has poor specificity, cumbersome operation, long detection cycle, and large error in results
The disadvantages of ELISA detection are that peroxidase or alkaline phosphate markers are easily inactivated, and the chromogenic substrate is easily decomposed when exposed to light, with low sensitivity and certain cross-reactivity to compounds with similar structures, resulting in inaccurate test results. Accurate, and only suitable for the detection and identification of trace substances
The disadvantages of using high performance liquid chromatography are that the sample pretreatment is complicated, laborious and time-consuming, and the detection cost is high.
The disadvantage of using the colloidal gold method for detection is that the final detection result is characterized by the color visible to the naked eye, the error is large, and the operation is cumbersome, the process is more, the error is more prone to occur, and the sensitivity is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] The present invention provides a kit for detecting food safety by chemiluminescence and a preparation method thereof, wherein, the kit for detecting the residues of oxaconazole pesticide by chemiluminescence method provided by the present invention can use oxaconazole antibody coupled magnetic Microparticles, acridinium ester-labeled oxaflucondone, can also use acridinone antigen-coupled magnetic particles, acridinium ester-labeled oxaflucondone antibody. The kit also includes oxaconazole calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The oxaconazole antigen is a synthetic polypeptide antigen, and its amino acid sequence is Glu Lys Lys Gly Ile Gln Gly SerThr Ala Lys Ser.

[0023] Specifically, in the process of preparing the magnetic particle coupling of the present invention, the buffer solution of the coupling antigen is a buffer solution with a pH value of 6.0 and a concentration of 0.1mol / L MES...

Embodiment 1

[0030] Example 1: The composition and preparation method of a kit 1 for detecting food safety by chemiluminescence 1. The composition of the kit 1

[0031] Acridinium ester-labeled oxafluconone antigen;

[0032] Magnetic particle-coupled oxaflucondone antibody;

[0033] Oxacontazone series calibrator solutions, concentrations are: 0μg / L, 0.4μg / L, 2μg / L, 10 μg / L, 50μg / L, 250μg / L;

[0034] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0035] The cleaning solution includes the following components: a trometamol solution with a pH value of 7.2 and a concentration of 25 mmol / L, which contains sodium chloride at a concentration of 0.15 mmol / L and Tween 20 with a mass fraction of 0.05%.

[0036] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Oxaflucondone Antibody

[0037] (1) Take 1mg of carboxyl magnetic particles in a 0.5mL centrifuge tube, add a certain amount of 0.1mol / L MES buffer, vortex and mix, place on a m...

Embodiment 2

[0054] Example 2: The composition and preparation method of a kit 1 for detecting food safety with chemiluminescence

[0055] 1. Construction of Kit 2

[0056] Acridinium ester-labeled oxafluconone antigen;

[0057] Magnetic particle-coupled oxaflucondone antibody;

[0058] Oxacontazone series calibrator solutions, concentrations are: 0μg / L, 0.4μg / L, 2μg / L, 10 μg / L, 50μg / L, 250μg / L;

[0059] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0060] The cleaning solution includes the following components: a trometamol solution with a pH value of 7.2 and a concentration of 25 mmol / L, which contains sodium chloride at a concentration of 0.15 mmol / L and Tween 20 with a mass fraction of 0.05%.

[0061] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Oxaflucondone Antibody

[0062] (1) Take 1mg of carboxyl magnetic particles in a 0.5mL centrifuge tube, add a certain amount of 0.1mol / L MES buffer, vortex and mix, place o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a kit for detecting the food safety by chemiluminescence and a preparation method of the kit. The kit comprises an acridinium ester labeled famoxadone antigen or antibody, a magnetic micro-particle coupled antibody or antigen, a famoxadone series standard product solution, a chemiluminescence pre-excitation solution A, a chemiluminescence pre-excitation solution B and a washing solution. The kit disclosed by the invention combines a chemiluminescence technology and immune magnetic micro-particles and a reaction system close to a homogeneous phase is provided. Compared with the prior art, the kit disclosed by the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a kit for detecting oxaflucone residues in food safety by using chemiluminescence and a preparation method thereof. Background technique [0002] Famoxadone, foreign name Famoxadone, chemical formula is C22H18N2O4. Oxaflucone is a new type of high-efficiency, broad-spectrum bactericidal pesticide. Suitable crops such as wheat, barley, peas, sugar beets, rapeseed, grapes, potatoes, claws, peppers, tomatoes, etc. It is mainly used to control important diseases in Ascomycetes, Basidiomycetes, and Oomycetes, such as powdery mildew, rust, glume blight, net spot, downy mildew, and late blight. Mixed with flusilazole, it is more effective in preventing and controlling wheat blight, net spot, powdery mildew, and rust, and has protection, treatment, eradication, penetration, and systemic activities. The mechanism of action is an energy inhibitor, that is, inhibition of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76
CPCG01N33/54326G01N21/76
Inventor 李梅秀
Owner 李梅秀
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com