Spray drying production process and application of high-activity lactobacillus powder

A technology of lactobacillus and bacteria powder, applied in the direction of lactobacillus, application, bacteria, etc., can solve the problems of limited improvement, unsuitability for industrial application, cumbersome operation steps, etc., to improve functions, improve self-stress resistance, and increase survival rate Effect

Inactive Publication Date: 2018-05-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the optimization of drying protectant formula, the improvement of drying process conditions, and the application of microencapsulation technology can improve the drying survival rate of La

Method used

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  • Spray drying production process and application of high-activity lactobacillus powder

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the spray drying of Lactobacillus reuteri

[0033] (1) Preparation of Lactobacillus film fermentation medium:

[0034] Preparation of Lactobacillus reuteri membrane fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish powder peptone 10g / L, magnesium sulfate heptahydrate 0.5 g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween- 801g / L, the amount of insoluble fiber added is shown in Table 1.

[0035] (2) Fermentation preparation of Lactobacillus film: activate Lactobacillus reuteri for 3 generations, transfer it to a 10L fermenter according to the inoculation ratio of 3-5%, continuously feed nitrogen, adjust the rotation speed, and keep the pH constant at 37°C (pH=6) and cultivated for 24 hours.

[0036] (3) Collection of Lactobacillus pellicle: Centrifuge the Lactobacillus...

Embodiment 2

[0043] (1) Preparation of Lactobacillus film fermentation medium:

[0044] Preparation of Lactobacillus reuteri membrane fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish powder peptone 10g / L, magnesium sulfate heptahydrate 0.5 g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween- 801g / L, soybean powder 10g / L.

[0045] (2) Fermentation preparation of Lactobacillus film: activate Lactobacillus reuteri for 3 generations, transfer it to a 10L fermenter according to the inoculation ratio of 3-5%, continuously feed nitrogen, adjust the rotation speed, and keep the pH constant at 37°C (pH=6) and cultivated for 24 hours.

[0046] (3) Collection of Lactobacillus pellicle: Centrifuge the Lactobacillus pellicle fermentation liquid at 4°C to collect the sludge. The centrifugation conditions are: the ro...

Embodiment 3

[0050] Embodiment 3: the spray drying of lactobacillus rhamnosus

[0051] (1) Preparation of Lactobacillus film fermentation medium:

[0052] Yeast powder 6g / L, anhydrous sodium acetate 4g / L, beef extract 12g / L, anhydrous glucose 25g / L, peptone 12g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween-801g / L, soybean flour 10g / L, oat bran Powder 10g / L.

[0053] (2) Fermentation preparation of Lactobacillus pellicle: Activate 3 generations of Lactobacillus, transfer it to a 10L fermenter according to the inoculation ratio of 3-5%, continuously feed nitrogen, adjust the rotating speed, and constant pH at 37°C (pH= 6) Culture for 24 hours.

[0054] (3) Collection of Lactobacillus pellicle: Centrifuge the Lactobacillus pellicle fermentation liquid at 4°C to collect the sludge. The centrifugation conditions are: the rotation sp...

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Abstract

The invention discloses a spray drying production process and application of high-activity lactobacillus powder, and belongs to the technical field of food. Lactobacillus biofilm thalli are produced by fermenting water-insoluble dietary fiber as a solid carrier; bacterial sludge collected after fermentation and a drying protective agent are mixed and subjected to spray drying, and the lactobacillus powder is prepared. The survival rate of spray dried lactobacillus with the method is 50% or higher, the highest viable bacteria number of the bacterial powder can reach 5*10<10> cfu/g or more, andthe survival rate is increased by 50 times or higher compared with that of the spray dried lactobacillus in a non-biofilm state. The key common technical problem of developing high-activity lactobacillus powder is solved, and the prepared lactobacillus powder can be applied to product forms such as fermentation agents, solid beverages and the like.

Description

technical field [0001] The invention relates to a spray-drying production process and application of highly active Lactobacillus powder, belonging to the technical field of food. Background technique [0002] Lactobacillus is an inherent flora in the intestines of humans and animals, and plays an important role in the stability of intestinal microecology and the regulation of host physiological functions. Commercially available live Lactobacillus products include: Lactobacillus solid drinks, Lactobacillus fermented milk products, etc. Most of these products are made from Lactobacillus powder. At present, most of the commercially available Lactobacillus powders are produced by freeze-drying. Although freeze-drying can obtain Lactobacillus powders with a high survival rate, freeze-drying has the disadvantages of high production equipment requirements, high energy consumption during the drying process, and Shortcomings such as low yield, these problems have greatly improved th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23C9/123A23G1/42A23G3/36A23L2/39A23L29/00A23L33/135C12R1/225C12R1/245C12R1/25
CPCA23C9/123A23G1/423A23G3/366A23L2/39A23L29/065A23L33/135C12N1/04C12N1/20A23V2002/00A23V2400/145A23V2400/151A23V2400/137A23V2400/147A23V2400/143A23V2400/125A23V2400/181A23V2400/173A23V2400/113A23V2400/175A23V2400/169A23V2200/30
Inventor 陈卫陆文伟翟齐啸崔树茂赵建新张灏
Owner JIANGNAN UNIV
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