Method for improving stress resistance of streptococcus thermophilus and application thereof in preparation of streptococcus thermophilus powder

A technology of Streptococcus thermophilus and bacterial powder, applied in the food field, can solve the problems of unsuitable industrial application, cumbersome operation steps, limited improvement degree, etc., and achieve the effect of improving self-resistance, high activity, and strong resistance to stress.

Pending Publication Date: 2018-06-01
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the optimization of drying protectant formula, the improvement of drying process conditions, and the application of microencapsulation technology can improve the drying survival rate of Streptococcus thermophilus to a certain extent, the improvement of the above methods is limited, and the operation steps are cumbersome, which is not suitable for large-scale industrial application of

Method used

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  • Method for improving stress resistance of streptococcus thermophilus and application thereof in preparation of streptococcus thermophilus powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Freeze-drying of Streptococcus thermophilus

[0032] (1) Preparation of streptococcus thermophilus membrane fermentation medium: yeast powder 5g / L, beef extract 5g / L, lactose 10g / L, tryptone 5g / L, soybean peptone 5g / L, magnesium sulfate 0.2g / L, Sodium glycerophosphate is 20g / L, ascorbic acid is 0.5g / L, and the amount of insoluble fiber added is shown in Table 1.

[0033] (2) Fermentation preparation of Streptococcus thermophilus bacterium film: Activate Streptococcus thermophilus for 3 generations, according to the inoculation ratio of 3-5%, transfer in the 10L fermenter, adjust the rotating speed, 37 degrees of constant pH (pH=6.5 ) for 24 hours.

[0034] (3) Collection of Streptococcus thermophilus pellicle: Centrifuge the fermented liquid of Streptococcus thermophilus to collect the bacterium sludge at 4°C. Streptococcal slime.

[0035] (4) Add drying protective agent: Mix the obtained bacteria slime and drying protective agent according to the weight-t...

Embodiment 2

[0041] Follow the same steps as in Example 1, the difference is that soybean powder and oat bran powder are replaced by kudzu powder and walnut shell powder to carry out bacterial film fermentation, and the results of freeze-drying show that the kudzu root powder and walnut shell powder are used as solid media. The freeze-dried survival rate of the bacterial film was 87%, and the number of viable bacteria in the bacterial powder was 7×10 10cfu / g. The bacterial powder was stored at 25 degrees for 12 weeks, and the survival rate was 60%.

Embodiment 3

[0043] Follow the same steps as in Example 1, except that the freeze-drying protective agent is replaced by: 120g / L skimmed milk powder, 20g / L trehalose, 20g / L sucrose, 0.5g / L cysteine ​​hydrochloride, The pH is 6.5. The freeze-drying survival rate of pellicle-forming Streptococcus thermophilus was 83%, which was significantly higher than the freeze-drying survival rate of bacteria in the non-pellet state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 56%.

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Abstract

The invention discloses a method for improving stress resistance of streptococcus thermophilus and application thereof in preparation of streptococcus thermophilus powder and belongs to the technicalfield of food. According to the method, the streptococcus thermophilus powder is prepared through taking non-water-soluble dietary fibers as a solid-phase carrier, carrying out fermentation to producestreptococcus thermophilus biofilm thalli, then, collecting bacterium mud, mixing the collected bacterium mud with a drying protectant, and carrying out drying. The streptococcus thermophilus prepared by using the method disclosed by the invention has the advantages that the survival rate after drying is high, the survival rate after freeze drying can reach 85% or more, the survival rate after vacuum drying can reach 35% or more to the maximum, the survival rate after spray drying can reach 40% or more, and the viable count of the streptococcus thermophilus powder can reach 5*10<10>cfu/g or more. Compared with non-biofilm-state streptococcus thermophilus, the survival rate after drying is increased by 10 times or more. According to the method and the application, the technical problem, i.e., key generality in development of high-activity streptococcus thermophilus powder is solved, and the prepared streptococcus thermophilus powder can be applied to product forms such as fermentationagents and solid drinks.

Description

technical field [0001] The invention relates to a method for improving the stress resistance of streptococcus thermophilus and its application in the preparation of bacterial powder, belonging to the technical field of food. Background technique [0002] Streptococcus thermophilus is a typical dairy starter, mainly used in yogurt production. Streptococcus thermophilus produces lactic acid, exopolysaccharides and characteristic flavor substances by fermenting milk, which endows yogurt products with unique texture and flavor. [0003] Streptococcus thermophilus powder is usually prepared by vacuum freeze-drying technology. The survival rate of freeze-dried strains can be improved by optimizing the formula of the protective agent. At the same time, freeze-drying has the disadvantages of high production equipment requirements, high energy consumption during the drying process, and low yield. These problems have greatly increased the preparation cost of Streptococcus thermophilu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/46
CPCC12N1/04C12N1/20
Inventor 陆文伟陈卫翟齐啸崔树茂赵建新张灏
Owner JIANGNAN UNIV
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