Application of clove essential oil in sterilization and/or bacteria inhibition and drug-resistant bacteria prevention

A technology of clove volatile oil and drug-resistant bacteria, applied in the field of sterilization and/or bacteriostasis, clove volatile oil, anti-drug-resistant bacteria, can solve the problems that have not yet been seen in the study of clove volatile oil anti-drug-resistant bacteria

Inactive Publication Date: 2018-05-18
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant literature report on t

Method used

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  • Application of clove essential oil in sterilization and/or bacteria inhibition and drug-resistant bacteria prevention
  • Application of clove essential oil in sterilization and/or bacteria inhibition and drug-resistant bacteria prevention
  • Application of clove essential oil in sterilization and/or bacteria inhibition and drug-resistant bacteria prevention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Clove volatile oil antibacterial effect

[0022] 1 Materials and methods

[0023] 1.1 Materials:

[0024] (1) Tested strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa.

[0025] (2) Culture medium: nutrient broth medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.

[0026] 1.2 Experimental method

[0027] 1.2.1 Activation of strains: Take 3 clean test tubes, add 5 mL of nutrient broth medium respectively, seal with silica gel plugs, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 3 kinds of bacteria into the test tube, mix them evenly, and place them in the incubator for 18-24 hours to observe the results. Among them, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were placed in a 37°C incubator.

[0028] 1.2.2 Oxford cup method (tube and dish method) to screen the antibacterial activ...

Embodiment 2

[0033] Embodiment 2 Clove volatile oil antifungal effect

[0034] 1 Materials and methods

[0035] 1.1 Materials:

[0036] (1) Tested strain: Candida albicans.

[0037] (2) Culture medium: nutrient broth medium, improved Martin medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.

[0038] 1.2 Experimental method

[0039] 1.2.1 Activation of strains: Take a clean test tube, add 5 mL of modified Martin's medium, seal it with a silica gel plug, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 1 species of bacteria into the test tube, mix well, and place it in an incubator for 18-24 hours to observe the results. Place Candida albicans at 26.5°C in the incubator.

[0040] 1.2.2 Oxford cup method (tube and dish method) to screen the antibacterial activity of volatile oil of traditional Chinese medicine

[0041] Measure the OD600 value of the microbial ...

Embodiment 3

[0047] Embodiment 3 Clove volatile oil anti-drug-resistant bacteria effect

[0048] 1. Materials and methods

[0049] 1.1 Bacterial Strains

[0050]Drug-resistant Staphylococcus aureus (MRSA), bacterial number: BAA-1717; drug-resistant Klebsiella, bacterial number: BAA-1705; drug-resistant Pseudomonas aeruginosa, bacterial number: BAA-2110; Drug-resistant Enterococcus faecium, strain number: BAA-2320; drug-resistant Acinetobacter baumannii, strain number: 19606; both were purchased from the American Type Culture Collection (ATCC). Drug-resistant entero-invasive Escherichia coli, bacteria number: 186731, was purchased from Beijing Beina Chuanglian Biotechnology Research Institute. Beta-hemolytic streptococci were purchased from the China General Microorganism Culture Collection and Management Center.

[0051] 1.2 Test reagents

[0052] table 3

[0053]

[0054] 1.3 Test equipment

[0055] Table 4

[0056]

[0057] 1.4 Test method

[0058] Add tryptone liquid mediu...

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Abstract

The invention discloses application of bacteriostatic and drug-resistance bacteria prevention functions of clove essential oil. The clove volatile oil is extracted through a water vapor distillation method, and the clove volatile oil has certain killing and/or growth inhibition functions on bacterial and fungi such as staphylococcocci, escherichia coli, candida, pseudomonades, enterobacter and streptococcocci. The clove volatile oil has certain killing and/or growth inhibition functions on viruses such as drug-resistance staphylococcocci, drug-resistance escherichia coli, drug-resistance enterobacter coli, drug-resistance klebsiella, drug-resistance pseudomonades, drug-resistance acinetobacter, drug-resistant streptococcocci and drug-resistant enterococcocci. The clove volatile oil can beapplied to industries such as food, medicine, health-care products and cosmetics, and can be prepared into various products with the bacteriostatic and drug-resistance bacteria prevention functions. The invention belongs to the field of novel application of the clove volatile oil.

Description

technical field [0001] The invention relates to the technical field of food, medicine and chemical industry, in particular to the application of clove volatile oil for sterilization and / or bacteriostasis and resistance to drug-resistant bacteria. Background technique [0002] Clove Caryophylli Flos is the dried flower bud of Eugenia caryophyllata Thunb., a plant of the family Myrtleaceae. It is one of the key medicinal materials developed and researched in my country, and it is also one of the commonly used seasonings in daily food. It is a dual-purpose traditional Chinese medicine for medicine and food. It is warm in nature and pungent in taste. It has the effects of nourishing the kidney and yang, warming the middle and lowering the adverse. The main chemical component of clove is volatile oil, and its volatile oil is the material basis of various pharmacological effects. [0003] Literature (Qiu Qin, Cui Zhaojie, Zhao Yi. GC-MS analysis of chemical components of clove vo...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P31/04A61P31/10A61K8/92A61Q17/00A61Q19/00A23L3/3463
CPCA23L3/3463A23V2002/00A61K8/922A61K36/61A61K2236/331A61K2236/37A61K2800/10A61K2800/524A61Q17/005A61Q19/00A23V2250/21Y02A50/30
Inventor 郑琴杨明王雅琪梁新丽黄小英张小飞
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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