Streptomyces deccanensis QY-3 and application thereof

A technology of Streptomyces decanata, QY-3, applied in the application, bacteria, fungicides and other directions, to achieve the effect of broad-spectrum anti-phytopathogenic fungi activity and good control effect

Active Publication Date: 2018-05-18
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the currently screened biocontrol actinomycetes against anthracnose are aimed at yams, strawberries, citrus, grapes,

Method used

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  • Streptomyces deccanensis QY-3 and application thereof
  • Streptomyces deccanensis QY-3 and application thereof
  • Streptomyces deccanensis QY-3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and screening of Streptomyces deccanensis QY-3

[0027] Take glacier surface soil to obtain glacier sediment. Take 10g of glacier sediment sample, grind it fully, put it into a triangular flask with 100mL sterile water and glass beads inside, vibrate on a shaker at 100rpm for 20min, take the supernatant for 10 times Gradual dilution to obtain the dilution, take 0.1mL of the dilution and spread it evenly on the Gaussian No. 1 medium plate, place it in a constant temperature incubator at 28°C and cultivate it for 5-10 days, pick up actinomycetes colonies and further separate them by streaking Purify until the pure culture of actinomycete strains is obtained; with G. anthracnose as the target bacteria, a strain QY-3 with high inhibitory activity against G. anthracnose was screened by confrontation culture method.

[0028] The biological characteristics of Streptomyces deccanensis QY-3 of the present invention are as follows:

[0029] The effective frag...

Embodiment 2

[0042] Embodiment 2: the inhibitory activity determination of Streptomyces deccan QY-3 to pathogenic bacteria

[0043] Fermentation medium used in the present invention: millet 20g, peptone 3g, glucose 20g, CaCO 3 2g, NaCl 2.5g, water 1000mL, pH: 7.2-7.4, culture conditions are 28°C, 180rpm shaking culture for 7d.

[0044] The inhibitory activity of Streptomyces deccan QY-3 against G. anthracnose was determined by plate confrontation test. As shown in Figure 2, Streptomyces deccan QY-3 had strong antagonistic activity against G. anthracnose.

[0045] Streptomyces deccan QY-3 was fermented and cultured, and the supernatant of the fermentation broth was obtained by filtering out the bacteria, and its inhibitory activity against different plant pathogenic fungi was tested. As can be seen from Table 4, the fermented liquid of Streptomyces deccan QY-3 has broad-spectrum antifungal activity, especially to the pathogenic bacteria of the genus Anthracnose. 87.0% and 88.9%, respecti...

Embodiment 3

[0049] Embodiment 3: the temperature and pH stability determination of Streptomyces deccan QY-3 fermented liquid

[0050] Streptomyces deccan QY-3 fermentation broth was treated at different temperatures and pHs for 1 hour, and its inhibitory activity against G. anthracnose was determined. Depend on image 3 Shown, Streptomyces deccan QY-3 fermentation broth has better temperature and pH stability.

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Abstract

The invention discloses streptomyces deccanensis QY-3 and application thereof. The Latin name of the strain is streptomyces deccanensis, the number of the strain is QY-3, the strain is collected in CGMCC (China General Microbiological Culture Collection Center), and the collection number is CGMCC No.14467. The streptomyces deccanensis QY-3 disclosed by the invention has broad-spectrum activity forresisting to plant pathogenic fungi, has obvious inhibition effects on plant pathogenic fungi such as colletotrichum gloeosporioides, colletotrichum musae, banana fusarium oxysporum, fusarium graminerum, cucumber fusarium wilt, dragon fruit fusarium, dragon fruit rot pathogens, physalospora piricola, phomopsis mangiferae or guava pestalotiopsis, and especially has good prevention and treatment effects on colletotrichum gloeosporioides of rubber trees, so that the streptomyces deccanensis QY-3 is bio-control actinomyces strain with great development potential.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a strain of Streptomyces deccan QY-3 and its application. Background technique [0002] Rubber tree (Hevea brasiliensis) is a perennial tropical rainforest tree species, mainly distributed in Hainan, Yunnan, Guangdong, Guangxi, Fujian and other regions in China, and is the main source of natural rubber. Natural rubber is an important industrial raw material and strategic resource. It is closely related to people's daily life, national defense and industrial construction. Its superior performance cannot be completely replaced by synthetic rubber at present, and it occupies an important strategic position in the national economy. Rubber anthracnose is an important leaf disease that harms rubber trees in my country. It is one of the four major diseases in rubber production. The repeated defoliation and the withering of young shoots delayed the opening and cutting ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/02A01P3/00C12R1/465
CPCA01N63/10C12N1/02C12N1/20C12N1/205C12R2001/465
Inventor 李晓宇柳志强辜柳霜章程辉张楠张月凤吴曼莉
Owner HAINAN UNIVERSITY
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