Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lytic vibrio phage VspSw-1, bactericidal composition containing the same and application thereof

A phage and composition technology, applied in the field of Vibrio schisis phage VspSw-1 and the bactericidal composition containing the phage, can solve the problems of easy resistance of host bacteria, short duration of lysis ability, narrow lysis host spectrum, etc. , to achieve the effect of low production cost, good control effect and high potency

Active Publication Date: 2018-05-18
SHENZHEN INST OF ADVANCED TECH
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lytic vibrio phage VspSw-1, bactericidal composition containing the same and application thereof
  • Lytic vibrio phage VspSw-1, bactericidal composition containing the same and application thereof
  • Lytic vibrio phage VspSw-1, bactericidal composition containing the same and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0093] The present invention separates and obtains 7 strains of pathogenic Vibrio from its lesions by collecting environmentally friendly fish and shrimp samples in the water samples of Shanwei prawn breeding grounds. The screening flow chart of the bacteriophage of the present invention is as follows figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were identified as highly pathogenic, LD 50 4 CFU / mL, action time <100h.

[0094] Through the plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 32 strains of different species (Table 1) was determined, and it was concluded that the phage has strong host specificity and is a Vibrio phage. Is a specific split phage.

[0095] Table 1 host spectrum

[0096]

[0097]

[0098] Remarks: "+" means it can be lysed, "-" means it can't be lysed; the above strains are all isolated from aquaculture fish...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a lytic vibrio phage VspSw-1, a bactericidal composition containing the same and application thereof. The phage can rapidly inhibit vibrio growth within a short time, and theinhibiting effect lasts for a long time. In addition, the bactericidal composition of the phage is safe and effective, also has strong specificity, is low in production cost, and makes up for the shortcomings of single phage therapy in the control process of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio schisis phage VspSw-1, a bactericidal composition containing the phage and applications thereof. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important pat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00032C12N2795/00021A61K2300/00Y02A50/30
Inventor 马迎飞樊继强王婧婷陈玲刘陈立
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com