Lytic vibrio phage ValLY-4, bactericidal composition containing the same and application thereof

A vally-4 and phage technology, applied in the biological field, can solve the problems of easy resistance of host bacteria, short duration of lysis ability, narrow lysis host spectrum, etc., and achieve low production cost, good control effect and high potency Effect

Inactive Publication Date: 2018-05-18
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

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  • Lytic vibrio phage ValLY-4, bactericidal composition containing the same and application thereof
  • Lytic vibrio phage ValLY-4, bactericidal composition containing the same and application thereof
  • Lytic vibrio phage ValLY-4, bactericidal composition containing the same and application thereof

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Embodiment

[0093] The present invention separates and obtains 7 strains of pathogenic Vibrio from its lesions by collecting environmentally friendly fish and shrimp samples in the water samples of Shanwei prawn breeding grounds. The screening flow chart of the bacteriophage of the present invention is as follows figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were identified as highly pathogenic, LD 50 4 CFU / mL, action time <100h.

[0094] By plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 32 strains of different species was determined (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio phage. Is a specific split phage.

[0095] Table 1 host spectrum

[0096]

[0097]

[0098] Remarks: "+" means it can be lysed, "-" means it can't be lysed; the above strains are all isolated from aquaculture fish and shr...

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Abstract

The invention relates to a lytic vibrio phage ValLY-4, a bactericidal composition containing the same and application thereof. The phage can rapidly inhibit vibrio growth within a short time, and theinhibiting effect lasts for a long time. In addition, the bactericidal composition of the phage is safe and effective, also has strong specificity, is low in production cost, and makes up for the shortcomings of single phage therapy in the control process of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio schisis phage ValLY-4, a bactericidal composition comprising the phage and applications thereof. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04
CPCA61K35/76C12N7/00C12N2795/00021C12N2795/00032A61K2300/00Y02A50/30
Inventor 马迎飞樊继强王婧婷陈玲刘陈立
Owner SHENZHEN INST OF ADVANCED TECH
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