Fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony

A Gram-negative bacteria and fluorescent probe technology, applied in the field of fluorescent probes, can solve problems such as limited usability, expensive AHL, and inability to withstand polymerization conditions, and achieve the effects of low price, high sensitivity, and simple operation

Inactive Publication Date: 2018-05-18
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But AHL is expensive, has limited availability and cannot withstand aggregation conditions

Method used

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  • Fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony
  • Fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony
  • Fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony

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Embodiment 1

[0028] The present invention is achieved through the following technical scheme, the present invention is dissolved in 60mL acetonitrile by furanone (DMHF) analogue template (0.1mmol) and methacrylic acid (MAA) monomer (0.4mmol), and stirred at 25 ℃ The mixture allows it to self-assemble. After 24 h, a certain amount of CdSe / ZnS quantum dots, ethylene glycol dimethacrylate (EGDMA) (1 mmol) and azobisisobutyronitrile (AIBN) (12 mg) were added and sonicated for 5 min, followed by N 2 Purge for 20 minutes. The mixture was magnetically stirred at 600 rpm in a 60°C water bath for 24 hours. After polymerization, the mixture was cooled to room temperature, the precipitate (QDs@MIP) was collected, template removal was accomplished by a combination of sonication and centrifugation under optimal experimental conditions, and QDs@MIP was dispersed in ethanol acidified with 0.5 mol / l hydrochloric acid , followed by sonication and centrifugation to collect the precipitate. This process w...

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Abstract

The invention discloses a fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony, and belongs to the technical field of rapid detection of pathogenic bacterium. According to the fluorescent probe, a quantum dot is connected with MIP through a surface molecular imprinting technology, and the purpose of accurate quantitative detection is achieved through a fluorescence value on the basis of high degree specificity detection and fluorescence. MIP polymer is precipitated and polymerized on the surface of an amino-silane functional group modified by a CdSe / ZnS quantum dot in advance, so that QDs@MIP is successfully synthesized. The invention further discloses a preparation method and the application of the molecular imprinting fluorescent probe. The fluorescent probe can realize indirect detection of part of Gram negative bacteria, has the advantages of sensitivity, speediness and high specificity, and is low in price, and the method has an important implication for food safety or health care facilities, and can be applied to the direct monitoring of Gram negative bacteria.

Description

technical field [0001] The invention relates to a fluorescent probe for detecting and quantifying signal molecules of Gram-negative bacteria populations, and belongs to the technical field of rapid detection of pathogenic bacteria. Background technique [0002] Bacteria currently pose a big problem worldwide, multiplying mainly in the form of multicellular colonies or biofilms onto all inert and biological surfaces. According to the World Health Organization, more than 60 percent of infections reported in hospitals are caused by bacterial resistance to antibiotics. As spontaneous signaling components of Gram-negative bacteria, acyl-homoserine lactones (AHLs) mediate population density-dependent gene expression in a variety of Gram-negative bacteria, and to date, they have been reported to cause food spoilage and Most diseases cause bacteria to modulate their behavior through quorum sensing. [0003] Quorum sensing is a mechanism by which bacteria mediate the expression of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486G01N21/6402
Inventor 孙秀兰单雪晴吉地皮付伟纪剑张银志路勇
Owner JIANGNAN UNIV
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