Method for ultralow-temperature preservation of winged bean seeds

A technology for ultra-low temperature preservation and quadrangular bean, which is applied in the preservation of plants, botanical equipment and methods, applications, etc., can solve the problems of non-permanent preservation, reduction, and vitality reduction, and achieve safe preservation, simple process, and stability. reliable results

Inactive Publication Date: 2018-05-22
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant seeds stored at room temperature need to be replanted every 5 years, because after 5 years of storage, the vitality of plant seeds decreases rapidly, only about 50%. In China, a national medium-term bank and national For the crop germplasm bank, the storage temperature of the national medium-term bank is about 4°C, and the relative humidity is less than 65%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take the Xiangleng bean No. 1 harvested in a certain place in Hunan, and dry it naturally, and use the B5 liquid culture solution containing 2.2mol / L glycerol and 1.3mol / L dimethyl sulfoxide to the four-lens bean seeds with a water content of about 11%. Treatment, after 20 minutes of treatment, take out the treated square bean seeds, and put them into the cryopreservation tube of B5 liquid culture medium containing 3.2mol / L glycerol, 1.7mol / L dimethyl sulfoxide and 0.6mol / L sucrose , incubate at room temperature for 30 minutes, and then put it into the gradient cooling box; put the gradient cooling box into a -70°C low-temperature refrigerator for gradient cooling, so that the temperature of the cooling box is at -45°C, and the cooling rate is -1°C / min , then take out the cooling box, put the cooling box into liquid nitrogen immediately, and store it at a low temperature in liquid nitrogen for 5 years, then take the cryopreservation tube out of the liquid nitrogen, place...

Embodiment 1

[0025] Take Xiangleng bean No. 1 harvested in a certain place in Hunan that year, and dry it naturally. Treat the four-lens bean seeds with a water content of about 11% with B5 liquid culture solution containing 2.2mol / L glycerol. After 20 minutes of treatment, take out the treated Lens bean seeds, then put into the cryopreservation tube of the B5 liquid culture medium containing 3.2mol / L glycerol, 1.7mol / L dimethyl sulfoxide and 0.6mol / L sucrose, and then put it into the gradient cooling box ;Put the gradient cooling box into a -70°C low-temperature refrigerator for gradient cooling, so that the temperature of the cooling box is at -45°C, and the cooling rate is -1°C / min, then take out the cooling box, and immediately put the cooling box into liquid nitrogen , after 5 years of cryopreservation in liquid nitrogen, the cryopreservation tube was taken out of the liquid nitrogen, placed at a room temperature of about 23°C, and thawed naturally at room temperature. The tap water o...

Embodiment 2

[0027] Take the Xiangleng bean No. 1 harvested in a certain place in Hunan, and dry it naturally, and use the B5 liquid culture solution containing 2.2mol / L glycerol and 1.3mol / L dimethyl sulfoxide to the four-lens bean seeds with a water content of about 11%. Treatment, after 20 minutes of treatment, take out the treated square bean seeds, and put them into the cryopreservation tube of B5 liquid culture medium containing 3.2mol / L glycerol, 1.7mol / L dimethyl sulfoxide and 0.6mol / L sucrose , incubate at room temperature for 30 minutes, and then store it in a low-temperature refrigerator at -5°C for 5 years. Take the cryopreservation tube out of the liquid nitrogen, place it at a room temperature of about 23°C, and thaw it naturally at room temperature. After thawing, The four-lens bean seeds in the frozen storage tube were taken out, and then washed four times with tap water or distilled water. After washing, the four-lens bean seeds were directly used for germination experiment...

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PUM

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Abstract

The invention provides a method for ultralow-temperature preservation of winged bean seeds. The method comprises the following steps: treating the winged bean seeds with a reagent A at room temperature for 20 to 60 minutes, and taking out the treated winged bean seeds; placing the treated wing bean seeds in a cryogenic vial containing a reagent B, performing incubating for 10 to 30 min at room temperature, and placing the cryogenic vial in a gradient freezing box; placing the gradient freezing box with the winged bean seeds in a cryogenic refrigerator, and keeping the temperature of the box between -35 DEG C to -55 DEG C and a cooling rate at -1 DEG C/min; and taking out the freezing box, immediately placing the freezing box in liquid nitrogen, and cryopreserving the winged been seeds in liquid nitrogen for use.

Description

technical field [0001] The invention relates to a method for ultra-low temperature preservation of four-lens bean seeds, and belongs to the technical field of germplasm resource preservation. Background technique [0002] Four-sided beans are also called winged beans, winged beans, four-cornered beans, four-twisted beans, carambola beans, tropical soybeans, dragon beans, emperor beans, fragrant dragon beans, etc. The pods are four-sided long cylinders with four corners, so they are called four-sided beans. Bean or quadrangular bean, with wrinkled edges, shaped like wings, also known as winged bean, winged bean, pods shaped like carambola, also known as carambola bean, is an annual or perennial winding herbaceous plant of the leguminous quadrangular bean genus. Its seeds, tubers, young pods, young leaves and flowers are all edible; vines and pod shells can be used as a feed with high protein, high nutrition and good palatability after drying and processing. Square beans cont...

Claims

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Application Information

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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 罗婵
Owner 长沙爱扬医药科技有限公司
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