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A chip-based photoderivatizer for aflatoxins and sulfonamides

A technology for sulfonamides and aflatoxins, applied in the field of optical detection, to achieve the effects of improving photoderivatization efficiency, improving detection sensitivity, and reducing costs

Active Publication Date: 2020-05-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For chip capillary electrophoresis, chip microfluidic analysis, micro flow injection analysis, capillary liquid chromatography, microcolumn liquid chromatography, post-column light derivatization of ultra-high pressure liquid chromatography, there is no nanoliter to microliter pool volume. Report on photoderivatizers

Method used

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  • A chip-based photoderivatizer for aflatoxins and sulfonamides
  • A chip-based photoderivatizer for aflatoxins and sulfonamides

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A post-column optical derivatizer for ultra-high performance liquid chromatography (UPLC), the excitation light source is 2 high-power ultraviolet LEDs with a central wavelength of 365nm, the divergence angle is 120°, and the rated current is 500mA; the distance between the ultraviolet LED light source and the chip derivation reaction channel is 3mm The chip substrate is made of highly transparent quartz glass, on which there are chip-derived reaction channels arranged in a comb shape. The channel is a rectangular channel with a width of 150 μm, a depth of 200 μm, and a length of 250 mm. The volume of the derived pool is 7.5 μL; the chip-derived reaction channel and The reflector distance is 3mm; the reflector is a stainless steel polished plate; the material of the LED substrate and heat sink is metal aluminum. The chip light derivatizer was coupled with a Waters e2475FLD type fluorescence detector (2 μL cell volume) to evaluate its performance. The liquid chromatograp...

Embodiment 2

[0037] As described in Example 1, the chip-type optical derivatizer was combined with a Waters e2475FLD fluorescence detector to analyze and detect four kinds of sulfa drugs. The excitation wavelength is 230nm, and the emission wavelength is 400nm.

[0038] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and Waters e2475FLD was not detected when the derivatizer was not connected; after connecting the chip-type light derivatizer of the present invention, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15 .

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Abstract

The present invention discloses a chip type light derivatizer for aflatoxins and sulfonamides. An ultraviolet light-emitting diode (LED) having a center wavelength between 280 and 380 nm as an excitation light source, high-transparent quartz, ultraviolet-transmitting glass, PDMS (Polydimethylsiloxane) or perfluoroethylene propylene copolymer (FEP) is used as a chip substrate, a rectangular or trapezoidal groove path having a width of 10 to 500 mu m, a depth of 10 to 260 mu m, and a length of 5 to 500 mm is fabricated on the chip substrate as a derivation reaction tank, the groove path is in linear or comb-like or sine-wave shaped arrangement, the volume of the derivation reaction tank is 5nL to 75mu L, and is only 1 / 13 to 1 / 100000 of the volume of the derivation reaction tank of a conventional derivatizer. The chip type light derivatizer improves the fluorescence intensity of aflatoxin B1 by 6.5 times, and is suitable for post-column light derivation of chip capillary electrophoresis,chip microfluidic analysis, capillary liquid chromatography, microcolumn liquid chromatography, ultrahigh pressure liquid phase chromatography and normal liquid chromatography, and micro flow injection analysis / small flow injection analysis.

Description

technical field [0001] The patent of the present invention relates to the field of optical detection of microfluidic chemical analysis, capillary high performance liquid chromatography (μ-HPLC), and microfluidic injection analysis (μ-FIA), more specifically, a method for aflatoxin and sulfonamide Photoderivatizers for drug-like compounds. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfonamides by high-performance liquid chromatography (HPLC)-fluorescence detection, such as national standards GB / T 18979-2003 and GB / T 23212-2008, and the 2015 Chinese Pharmacopoeia The photoderivatizer is added to the chapter on aflatoxin determination to assist in the determination of trace aflatoxins in medicinal materials, decoction pieces and preparations. With the development of the times and the advancement of science and technology, national standards and EU testing standards have gradually reduced the limit values ​​o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N30/06G01N30/74
CPCG01N21/6428G01N30/06G01N30/74
Inventor 关亚风耿旭辉王楠
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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