Dog procalcitonin source detection kit and preparation method of fluorescent reagent in detection kit

Abstract

The invention relates to a dog procalcitonin source detection kit and a preparation method of a fluorescent reagent in the detection kit. The detection kit comprises a locking cover with a built-in reaction module and a box body with a built-in reagent module, the reaction module comprises a sample feeding hole, a reaction key, a reaction zone, a test paper plate and a barb; and the reagent modulecomprises a reagent groove, and the fluorescent reagent is arranged in the reagent groove. The dog procalcitonin source detection kit can be used for the quick quantitative analysis for CPCT in serum, plasma, peripheral blood or venous blood and has the advantages of high sensitivity, high specificity, good repetition and the like; and the dog procalcitonin source detection kit has good stability, linearity and accuracy, can rapidly, simply and quantitatively determine the content of an object to be detected, provides support for the diagnosis, identification diagnosis, treatment effect monitoring and healing evaluation of bacterial infection, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid quantitative detection kit for canine procalcitonin. Background technique [0002] Canine procalcitonin (Canine Procalcitonin, CPCT) is a non-hormonally active precursor of calcitonin and a major acute phase protein (APP) in dogs. From a single-copy gene located on chromosome 11 (llp15, 4), it is a glycoprotein consisting of 116 amino acids and a molecular weight of 13KD. PCT is composed of N-terminal-calcitonin-C-terminal three parts, which will not be degraded into calcitonin and will not be affected by hormone levels in the body. PCT is expressed by intraneural cells (including thyroid, lung and pancreatic tissue cells) after being stimulated by pro-inflammatory responses, especially those caused by bacteria, with a half-life of 25-30 hours. One of the good indicators. [0003] CPCT is an important marker that can specifically distinguish bacterial infection from other ...

AI Technical Summary

Problems solved by technology

RIA has a high detection sensitivity, but due to the radioactive hazards of the markers, the poor stability of the markers, and the difficulty of waste disposal, it has gradually withdrawn from the field of clinical testing; ELISA uses the principle of two double-antibody sandwich methods to detect CPCT in serum. The method is specific and has no cross-reaction, but the detection sensitivity is low, the minimum limit is only 10ug / L, the PCT concentration in normal serum cannot be detected, and the linear range is narrow, and it is not easy to realize full automation; GICA has the advantages of simple operation and high detection speed. However, it also has disadvantages such as low sensitivity, unstable reagents, poor repeatability, and difficulty in quantification; CLIA method is an immunoassay technology developed on the basis of RIAh and ELISA, which has high sensitivity and a linear range of detection. wide, easy to operate, and high degree of automation, but most of the chemiluminescence immunoassay kits in the prior art are imported closed automatic chemiluminescence detection systems, which require expensive automatic chemiluminescence detectors, thus limiting the promotion and use , cannot be widely used in clinical diagnosis and scientific research

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