Application of cell wall skeleton as penaeus vannamei feed additive
A technology of feed additive and cell wall, which is applied in the application field of cell wall skeleton as a feed additive of Penaeus vannamei, which can solve the problems of reduced disease resistance, frequent disease, and degradation of shrimp species, so as to increase the economic benefits of farming and improve the survival rate of farming , the effect of improving the body's immunity
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[0027] (2) Preparation of cell wall skeleton
[0028] Prepare the cell wall skeleton from the fermentation broth, the specific steps are:
[0029] 1) Centrifugation and washing
[0030] The fermentation broth was centrifuged to collect the bacteria, and the bacteria were washed with distilled water for 3 times;
[0031] 2) Preparation of coarse cell wall
[0032] Add distilled water to the cells, ultrasonically crush for 20 minutes, centrifuge and discard the sediment, centrifuge the supernatant again at 10,000 rpm, discard the supernatant, wash the sediment once with 0.9% normal saline and distilled water, and freeze-dry for 24 hours to obtain thick cell walls;
[0033] 3) Refined cell wall skeleton
[0034] Add trypsin and 0.07M, pH7.3 phosphate buffer to the above thick cell wall, stir slowly at room temperature for 24 hours, centrifuge, wash the precipitate twice with 0.07M phosphate buffer, repeat the above steps for the second enzymatic hydrolysis, The precipitate wa...
Embodiment 1
[0036] The application of a cell wall skeleton as a feed additive for Penaeus vannamei, adding 0.8 g of cell wall skeleton to 1 kg of Penaeus vannamei basal feed, and feeding Penaeus vannamei with an average body weight of 4.56 g for 4 weeks can significantly improve the hemolymph and hepatopancreas of Penaeus vannamei. The activities of superoxide dismutase, nitric oxide synthase, phenol oxidase, lysozyme and glutathione peroxidase in the blood (P<0.05, P<0.01), significantly reduced the concentration of malondialdehyde (P<0.05 , P<0.01), significantly enhanced the respiratory burst activity of shrimp hemolymph (P<0.05, P<0.01), significantly improved the immune protection rate of shrimp, and thus significantly improved the survival rate of Penaeus vannamei.
Embodiment 2
[0038] The application of a cell wall skeleton as a feed additive for Penaeus vannamei. Adding 1.0 g of cell wall skeleton to 1 kg of Penaeus vannamei basal feed and feeding Penaeus vannamei with an average body weight of 5.12 g for 4 weeks can significantly improve the hemolymph and hepatopancreas of Penaeus vannamei. The activities of superoxide dismutase, nitric oxide synthase, phenol oxidase, lysozyme and glutathione peroxidase in the blood (P<0.05, P<0.01), significantly reduced the concentration of malondialdehyde (P<0.05 , P<0.01), significantly enhanced the respiratory burst activity of shrimp hemolymph (P<0.05, P<0.01), significantly improved the immune protection rate of shrimp, and thus significantly improved the survival rate of Penaeus vannamei.
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