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Application of cell wall skeleton as penaeus vannamei feed additive

A technology of feed additive and cell wall, which is applied in the application field of cell wall skeleton as a feed additive of Penaeus vannamei, which can solve the problems of reduced disease resistance, frequent disease, and degradation of shrimp species, so as to increase the economic benefits of farming and improve the survival rate of farming , the effect of improving the body's immunity

Active Publication Date: 2018-06-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the passage of breeding time, the shrimp species degenerated significantly, and the resistance to diseases decreased, coupled with the deterioration of the breeding environment, resulting in frequent occurrence of diseases, which became the main bottleneck restricting the healthy development of my country's shrimp farming industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] (2) Preparation of cell wall skeleton

[0028] Prepare the cell wall skeleton from the fermentation broth, the specific steps are:

[0029] 1) Centrifugation and washing

[0030] The fermentation broth was centrifuged to collect the bacteria, and the bacteria were washed with distilled water for 3 times;

[0031] 2) Preparation of coarse cell wall

[0032] Add distilled water to the cells, ultrasonically crush for 20 minutes, centrifuge and discard the sediment, centrifuge the supernatant again at 10,000 rpm, discard the supernatant, wash the sediment once with 0.9% normal saline and distilled water, and freeze-dry for 24 hours to obtain thick cell walls;

[0033] 3) Refined cell wall skeleton

[0034] Add trypsin and 0.07M, pH7.3 phosphate buffer to the above thick cell wall, stir slowly at room temperature for 24 hours, centrifuge, wash the precipitate twice with 0.07M phosphate buffer, repeat the above steps for the second enzymatic hydrolysis, The precipitate wa...

Embodiment 1

[0036] The application of a cell wall skeleton as a feed additive for Penaeus vannamei, adding 0.8 g of cell wall skeleton to 1 kg of Penaeus vannamei basal feed, and feeding Penaeus vannamei with an average body weight of 4.56 g for 4 weeks can significantly improve the hemolymph and hepatopancreas of Penaeus vannamei. The activities of superoxide dismutase, nitric oxide synthase, phenol oxidase, lysozyme and glutathione peroxidase in the blood (P<0.05, P<0.01), significantly reduced the concentration of malondialdehyde (P<0.05 , P<0.01), significantly enhanced the respiratory burst activity of shrimp hemolymph (P<0.05, P<0.01), significantly improved the immune protection rate of shrimp, and thus significantly improved the survival rate of Penaeus vannamei.

Embodiment 2

[0038] The application of a cell wall skeleton as a feed additive for Penaeus vannamei. Adding 1.0 g of cell wall skeleton to 1 kg of Penaeus vannamei basal feed and feeding Penaeus vannamei with an average body weight of 5.12 g for 4 weeks can significantly improve the hemolymph and hepatopancreas of Penaeus vannamei. The activities of superoxide dismutase, nitric oxide synthase, phenol oxidase, lysozyme and glutathione peroxidase in the blood (P<0.05, P<0.01), significantly reduced the concentration of malondialdehyde (P<0.05 , P<0.01), significantly enhanced the respiratory burst activity of shrimp hemolymph (P<0.05, P<0.01), significantly improved the immune protection rate of shrimp, and thus significantly improved the survival rate of Penaeus vannamei.

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Abstract

The invention relates to an application of a cell wall skeleton as a penaeus vannamei feed additive. According to the application, 0.8-1.2g of the cell wall skeleton is added to each kilogram of a penaeus vannamei basal feed, wherein the cell wall skeleton is obtained in the following manner of enabling separated and purified nocardiopsis lucentensis to be subjected to enlarged culture, performingultrasonic wave crushing on fermentation liquor, performing centrifuging, performing enzymolysis with trypsin, performing defatting, and performing freeze drying so as to obtain a white cell wall skeleton. According to the application disclosed by the invention, the activity of superoxide dismutase, nitricoxide synthase, phenoloxidase, lysozyme and glutathione peroxidase in hemolymph and hepatopancreas of penaeus vannamei can be notably increased, the concentration of malondialdehyde can be notably reduced, the respiratory burst activity of the hemolymph of the penaeus vannamei can be notablystrengthened, the immunity protection ratio of the penaeus vannamei can be obviously increased, and the breeding survival rate of the penaeus vannamei can be obviously increased.

Description

technical field [0001] The invention belongs to the technical field of aquatic product breeding feed, in particular to the application of a cell wall skeleton as a feed additive for Penaeus vannamei. Background technique [0002] Penaeus vannamei, native to the waters of the Pacific coast of the Americas, is mainly distributed in the northern part of Peru to the coast of the Gulf of Mexico, with the most concentrated distribution along the coast of Ecuador. The Institute of Oceanology, Chinese Academy of Sciences introduced it to China from Hawaii, USA in 1988, and now it has been widely cultivated throughout the country. However, with the passage of breeding time, the shrimp species have degenerated significantly, and the resistance to diseases has decreased. Coupled with the deterioration of the breeding environment, diseases have occurred frequently, which has become the main bottleneck restricting the healthy development of my country's shrimp farming industry. Since ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K50/80C12N1/20C12N13/00C12R1/365
CPCA23K10/18A23K50/80C12N1/20C12N13/00
Inventor 袁春营孟阳吴佩佩崔青曼
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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