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Method for preparation of phosphatidic acid from micro-aqueous phase

A technology of phosphatidic acid and micro-water, applied in the field of phospholipids, can solve the problems of complicated preparation operation, long preparation period, and many wastes, etc., and achieve the effects of simple process steps, low cost and energy consumption, and less waste generation.

Active Publication Date: 2018-06-01
江苏中酶生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods generally have the disadvantages of cumbersome preparation operations, long preparation cycle, large waste, large energy consumption, and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Use 0.1kg lecithin (liquid phospholipid with 50% or more acetone insoluble content) as raw material, 0.005kg water as reaction solvent, after the two are fully mixed, add 0.005kg highly active phospholipase D concentrated enzyme solution at 55°C Carry out an enzyme-catalyzed reaction, supply 0.005kg of water to the reaction system after 12 hours, add 0.12kg of food-grade absolute ethanol to the reaction material after continuing to react for 12 hours, stir vigorously to precipitate solid insolubles, remove ethanol by centrifugation, and add water to the obtained precipitate After dissolving, add 0.16 kg of food-grade acetone and stir vigorously to precipitate solid insolubles. The acetone is removed by centrifugation, and the obtained solid can be dried in a vacuum to obtain a high-content phosphatidic acid product. HPLC detection shows that the phosphatidic acid content is 64.2%.

Embodiment 2

[0029] Use 1kg of lecithin (liquid phospholipid with acetone insoluble content greater than or equal to 50%) as raw material, 0.08kg of water as the reaction solvent, after the two are fully mixed, add 0.05kg of highly active phospholipase D concentrated enzyme solution at 55 ° C Enzyme-catalyzed reaction, after 12 hours, supply 0.08kg of water to the reaction system, continue to react for 12 hours, add 2.4kg of food-grade absolute ethanol to the reaction material, stir vigorously to precipitate solid insoluble matter, centrifuge to remove ethanol, and dissolve the obtained precipitate in water Add 2.0 kg of food-grade acetone and stir vigorously to precipitate solid insolubles, centrifuge to remove acetone, and vacuum-dry the obtained solid to obtain a high-content phosphatidic acid product. HPLC detection shows that the phosphatidic acid content is 68.2%.

Embodiment 3

[0031] Use 50kg of lecithin (liquid phospholipid with acetone insoluble content greater than or equal to 50%) as raw material, 2.5kg of water as the reaction solvent, after the two are fully mixed, add 2.5kg of highly active phospholipase D concentrated enzyme solution at 65 ° C Enzyme-catalyzed reaction, after 12 hours, supply 2.5kg of water to the reaction system, continue to react for 12 hours, add 120kg of food-grade absolute ethanol to the reaction material, stir vigorously to precipitate solid insoluble matter, centrifuge to remove ethanol, add water to dissolve the obtained precipitate, and then add 100kg of food-grade acetone was vigorously stirred to precipitate solid insolubles, and the acetone was removed by centrifugation. The resulting solid was vacuum-dried to obtain a high-content phosphatidic acid product. HPLC detection showed that the phosphatidic acid content was 72.7%.

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Abstract

The invention discloses a method for preparation of phosphatidic acid from a micro-aqueous phase. The method includes the steps of: adopting lecithin as the raw material, taking water as the reactionsolvent, fully mixing the two evenly to form a micro-aqueous phase system, adding highly active phospholipase D concentrated enzyme liquid and carrying out enzymic catalytic reaction at certain temperature for certain period of time, then adding food grade anhydrous ethanol into the reaction materials, performing intense stirring to precipitate out insoluble solid, conducting centrifugation to remove ethanol, adding water into the obtained precipitate for dissolving, then adding an appropriate volume of food grade acetone, and performing intense stirring to precipitate out insoluble solid, conducting centrifugation to remove acetone, and subjecting the obtained solid to vacuum drying so as to obtain a high content phosphatidic acid product. The method provided by the invention has the advantages of simple process, short production cycle, few waste and low cost, and is easy to realize industrial scale continuous production.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, and relates to a method for preparing phosphatidic acid in a slightly aqueous phase. Background technique [0002] Phosphatidic acid (Phosphatidic acid, PA), scientific name 3-phospho-1,2-diglyceride, is a phosphate-containing monolipid derivative and belongs to natural lipid compounds. As the basic components of animal and plant cell membranes, nuclear membranes, and lipid membranes, it is the basic substance of living organisms. It has the function of regulating the activity of various enzymes, and can also be used as a natural emulsifier, bitterness inhibitor, drug carrier, lipid messenger, etc. in food, medicine, cosmetics, fitness and other fields. From the chemical structure point of view, PA is an amphoteric compound, one end of the fatty acid group has strong lipophilicity, and one end of the phosphoric acid group has strong hydrophilicity. Phosphatidic acid is an intermediate in ...

Claims

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Application Information

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IPC IPC(8): C12P7/62
CPCC12P7/62
Inventor 严明蒋红宇陈圣章志林魏淼陈晶晶
Owner 江苏中酶生物科技有限公司