Application of 1,8-Naphthalimide Derivatives as Substrates in maldi-ms

A technology of naphthalene dicarboximide and derivatives, applied in the field of mass spectrometry analysis, can solve the problems of increasing the complexity of the experiment, affecting the properties of the analyte, etc., and achieves the effects of wide range of use, strong repeatability and less dosage
CN108132295BActive Publication Date: 2020-12-01DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2020-12-01

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Abstract

The invention discloses the application of N-butyl-4-hydroxyl-1,8-naphthalimide and its analogs as MALDI substrates, wherein N-butyl-4-hydroxyl-1,8-naphthalene Dicarboximide analogs include natural and synthetic compounds based on naphthalimide. This method is used to detect various types of compounds such as small molecules and polypeptides, including pure substances, mixture systems of various structurally similar compounds, mixture systems of various types of compounds, and mixture systems with other matrices. N-Butyl-4-Hydroxy-1,8-Naphthalimide has a uniform crystal shape and can be applied to tissue imaging experiments, which is conducive to obtaining high-resolution images and plays an important role in the quantitative analysis of analytes . In addition, the amount of N-butyl-4-hydroxy-1,8-naphthalimide substrate is small, and the ionization efficiency is high. N-butyl-4-hydroxy-1,8-naphthalimide As a commonly used rhodamine-based fluorescent dye, it can replace fluorescently labeled proteins and be used in the mechanism research of matrix-assisted laser desorption ionization mass spectrometry.
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Description

technical field

[0001] The invention relates to the application of N-butyl-4-hydroxy-1,8-naphthalimide and its analogues as substrates in matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), which belongs to mass spectrometry field. Background technique

[0002] Since the report of Karas et al. and Tanaka et al. in 1988, matrix-assisted laser desorption ionization mass spectrometry has attracted widespread attention because of its fast, high accuracy, and high sensitivity. It has been widely used in proteins, peptides, and nucleosides. During the analysis of biomolecules such as acids, polysaccharides, and amino acids.

[0003] Matrix-assisted laser desorption ionization is a soft ionization technology. Its principle is to irradiate the co-crystal film formed by the sample and the matrix with laser light. The matrix absorbs energy from the laser and transfers it to biomolecules. During the ionization process, protons are transferred to biomolecules or b...

Claims

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