Application of 1,8-Naphthalimide Derivatives as Substrates in maldi-ms

A technology of naphthalene dicarboximide and derivatives, applied in the field of mass spectrometry analysis, can solve the problems of increasing the complexity of the experiment, affecting the properties of the analyte, etc., and achieves the effects of wide range of use, strong repeatability and less dosage

Active Publication Date: 2020-12-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these studies use labeled proteins as analytes, and these labeling reagents may affect the properties of the analyte and increase the complexity of the experiment

Method used

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  • Application of 1,8-Naphthalimide Derivatives as Substrates in maldi-ms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 1 mg of N-butyl-4-hydroxy-1,8-naphthalimide and dissolve it in 1 ml of a mixed solution of 50% acetonitrile and water containing 0.2% trifluoroacetic acid, sonicate for 5 minutes, and collect the overgrowth after centrifugation The membrane supernatant was used for later use. Weigh 1 mg of acetyl-L-carnitine hydrochloride and dissolve it in 1 ml of methanol, and sonicate for 5 minutes to obtain a clear solution of acetyl-L-carnitine hydrochloride. Dissolve 5 μL of the solution in 450 μL of methanol and dilute to 10 μg / ml for later use. Take 0.5 μL of N-butyl-4-hydroxy-1,8-naphthalimide solution on a clean MALDI target plate, place it in the air and wait for the solvent to evaporate naturally for MALDI-MS analysis. The results are shown in the attached figure 1 shown.

[0030] After N-butyl-4-hydroxy-1,8-naphthalimide solution was mixed with 10 μg / ml acetyl-L-carnitine hydrochloride solution in equal volumes, take 0.5 μL of the mixed solution on a clean MALDI targ...

Embodiment 2

[0032] Weigh 1 mg of N-butyl-4-hydroxy-1,8-naphthalimide and dissolve it in 1 ml of a mixed solution of 50% acetonitrile and water containing 0.2% trifluoroacetic acid, sonicate for 5 minutes, and collect the overgrowth after centrifugation The membrane supernatant was used for later use. Dissolve 500 μg of Aβ35-40 in 500 μl of methanol and sonicate for 5 minutes to obtain a clear Aβ35-40 solution. Dissolve 10 μL of this solution in 90 μL of methanol and dilute to 100 μg / ml for use.

[0033] After mixing equal volumes of N-butyl-4-hydroxy-1,8-naphthalimide solution and 100 μg / ml Aβ35-40 solution, take 0.5 μL of the mixed solution on a clean MALDI target plate and place in air MALDI-MS analysis was carried out after the solvent was naturally volatilized, and the results are shown in the attached image 3 shown. As can be seen from the attached figure, [M+Na] + 、[M+K] + , [M+2Na-H] + , [M+K+Na-H] + 、[M+2K-H] + , [M+2K+Na-2H] + The peaks are obvious, and the resolution and ...

Embodiment 3

[0035] Weigh 1 mg of N-butyl-4-hydroxy-1,8-naphthalimide and dissolve it in 1 ml of a mixed solution of 50% acetonitrile and water containing 0.2% trifluoroacetic acid, sonicate for 5 minutes, and collect the overgrowth after centrifugation The membrane supernatant was used for later use. Dissolve 500 μg of Aβ35-42 in 500 μl of methanol and sonicate for 5 minutes to obtain a clear Aβ35-42 solution. Dissolve 10 μL of this solution in 90 μL of methanol and dilute to 100 μg / ml for use.

[0036] After mixing equal volumes of N-butyl-4-hydroxy-1,8-naphthalimide solution and 100 μg / ml Aβ35-42 solution, take 0.5 μL of the mixed solution on a clean MALDI target plate and place in air MALDI-MS analysis was carried out after the solvent was naturally volatilized, and the results are shown in the attached Figure 4 shown. As can be seen from the attached figure, [M+Na] + 、[M+K] + , [M+2Na-H] + The peaks are obvious, and the resolution and signal-to-noise ratio are high.

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Abstract

The invention discloses the application of N-butyl-4-hydroxyl-1,8-naphthalimide and its analogs as MALDI substrates, wherein N-butyl-4-hydroxyl-1,8-naphthalene Dicarboximide analogs include natural and synthetic compounds based on naphthalimide. This method is used to detect various types of compounds such as small molecules and polypeptides, including pure substances, mixture systems of various structurally similar compounds, mixture systems of various types of compounds, and mixture systems with other matrices. N-Butyl-4-Hydroxy-1,8-Naphthalimide has a uniform crystal shape and can be applied to tissue imaging experiments, which is conducive to obtaining high-resolution images and plays an important role in the quantitative analysis of analytes . In addition, the amount of N-butyl-4-hydroxy-1,8-naphthalimide substrate is small, and the ionization efficiency is high. N-butyl-4-hydroxy-1,8-naphthalimide As a commonly used rhodamine-based fluorescent dye, it can replace fluorescently labeled proteins and be used in the mechanism research of matrix-assisted laser desorption ionization mass spectrometry.

Description

technical field [0001] The invention relates to the application of N-butyl-4-hydroxy-1,8-naphthalimide and its analogues as substrates in matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), which belongs to mass spectrometry field. Background technique [0002] Since the report of Karas et al. and Tanaka et al. in 1988, matrix-assisted laser desorption ionization mass spectrometry has attracted widespread attention because of its fast, high accuracy, and high sensitivity. It has been widely used in proteins, peptides, and nucleosides. During the analysis of biomolecules such as acids, polysaccharides, and amino acids. [0003] Matrix-assisted laser desorption ionization is a soft ionization technology. Its principle is to irradiate the co-crystal film formed by the sample and the matrix with laser light. The matrix absorbs energy from the laser and transfers it to biomolecules. During the ionization process, protons are transferred to biomolecules or b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64G01N21/64
CPCG01N21/6428G01N27/64G01N2021/6439
Inventor 张晓哲程喜哪赵楠赵慧园
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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