Ratio-type near-infrared cysteine fluorescent probe

A compound and straight-chain technology, applied in fluorescence/phosphorescence, luminescent materials, material analysis through optical means, etc., can solve the problem of less biological imaging and achieve good stability

Active Publication Date: 2018-06-12
广煜医药科技(宁夏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many probes currently used to detect cysteine, but there are few ratiometric near-infrared fluorescent probes that can be used for biological imaging

Method used

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  • Ratio-type near-infrared cysteine fluorescent probe
  • Ratio-type near-infrared cysteine fluorescent probe
  • Ratio-type near-infrared cysteine fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Probe Preparation

[0044] Synthesis and Purification of 2,6-Diformylphenol

[0045] Use a balance to weigh 940.5mg of phenol and 2.80g of hexamethylenetetramine into a 100mL glass reaction bottle, add 30mL of trifluoroacetic acid, put in a clean small magnet, and then use 84-1 magnetic stirring control The warm electric heating mantle is heated to reflux, and the reaction time is recorded. During the reaction, the thin-layer chromatography silica gel plate is used to track the progress, and the reaction takes about 6 hours. After the reaction is complete, the reaction is turned off. The synthesis reaction is shown in formula I:

[0046]

[0047] Formula I: Synthesis of 2,6-diformylphenol

[0048] Prepare a 100mL beaker and pour about 50mL of distilled water into it. After the reaction stopped, after cooling, the reaction solution was added dropwise to the beaker prepared in advance,

[0049] Stir evenly with a dropper, pour the liquid in the beaker int...

Embodiment 2

[0072] Embodiment 2 solution preparation

[0073] Weigh 5.22 mg of the final product to prepare 1 mM probe stock solution. Weigh 12.1 mg of cysteine ​​to prepare 10 mM cysteine ​​(Cys) stock solution.

[0074]Pipette 50 μL of liquid from the probe mother solution and dilute to 10 mL to prepare a 5 μM probe detection solution. Divide 10 mL of the probe detection solution into two parts, and add 25 μL of the cysteine ​​mother solution to one of them at a concentration of 50 μM.

[0075] The above-mentioned systems for preparing solutions are ethanol:water=5:5 (v / v), containing PBS=5mM, pH=7.4.

Embodiment 3

[0076] Embodiment 3 The influence of response time on probe recognition cysteine

[0077] Prepare a 5 μM probe detection solution as described in Example 2, then quickly add cysteine ​​mother solution to make the concentration 10 μM, shake a few times, make the substances in the solution evenly distributed, and quickly put it into the fluorescence spectrophotometer In the meter, set the fluorescence spectrophotometer to scan once every 30 seconds, thereby obtaining the response spectrum at different times, record and save the spectrogram, and the room temperature during detection is 25°C. The detection solution system is still ethanol:water=5:5 (v / v) containing PBS=5mM, pH=7.4. The result is as figure 1 shown. It can be seen from the fluorescence spectrum that the peak value of the pure probe solution is relatively high at 700nm, and there is basically no peak at 530nm. The peak value gradually increased.

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PUM

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Abstract

The invention relates to a ratio-type near-infrared cysteine fluorescent probe that may act with cysteine and that has high sensitivity and selectivity. The ratio-type near-infrared cysteine fluorescent probe is applicable to high-sensitivity and high-selectivity recognition of cysteine or measurement of cysteine concentration in a sample.

Description

technical field [0001] The invention relates to a ratio-type near-infrared fluorescent probe for detecting cysteine. The probe of the present invention can interact with cysteine, and has the characteristics of high sensitivity and selectivity, and can be used in the identification of cysteine ​​with high sensitivity and high selectivity, or it can measure cysteine ​​in samples. concentration of amino acid. Background technique [0002] There are many types of thiols, but there are three small molecular biothiols, namely homocysteine, glutathione and cysteine. Among them, cysteine ​​exists in many small molecules and proteins in biological tissue cells, and is an important substance that composes them, and cysteine ​​plays a very important role in regulating various physiological and pathological processes in mammalian regulatory systems , mainly through the reducing properties of cysteine ​​can be oxidized to oxidative substances to maintain the redox balance in the cellu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D417/10C09K11/06G01N21/359G01N21/64
CPCC07D417/10C09K11/06C09K2211/1007C09K2211/1029C09K2211/1037G01N21/359G01N21/6428
Inventor 刘岩
Owner 广煜医药科技(宁夏)有限公司
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