Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for preparing a multifunctional microfluidic chip for in situ formation of embryoid bodies

A microfluidic chip, multi-functional technology, applied in biochemical equipment and methods, surface pretreatment bonding method, tissue cell/virus culture device, etc., can solve the problems of inability to directly observe the lower layer and limit applications

Active Publication Date: 2021-10-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional Transwell experiment cannot directly observe the phenomenon of the lower layer, it can only be observed through fluorescence, which limits its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing a multifunctional microfluidic chip for in situ formation of embryoid bodies
  • A method for preparing a multifunctional microfluidic chip for in situ formation of embryoid bodies
  • A method for preparing a multifunctional microfluidic chip for in situ formation of embryoid bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An in situ formation of multifunctional microfluidic chip in situ forming a multifunctional microfluidic chip, which is a Transwell microfluidic chip for cell migration and invasion experiments; the chip consists of three-layer chip, The upper layer chip is a cell culture chamber with a collapsed column. The intermediate layer is a porous filter film, and the lower layer is a cell culture chamber with a microarray column; the lower surface of the microfluidic chip is under the surface and a porous filter membrane For electrostatic adsorption, the upper surface of the underlying chip and the porous filter film are bonded to polymethylsiloxane PDMS, and the upper surface of the underlying chip and the lower surface of the top layer chip are bonded by polydimethylsiloxane PDMS;

[0032] The preparation method of the multi-function microfluidic chip forming the pyrocarbon bodies is in turn:

[0033] Take the porous filter in the position of the top chip to observe the window;

...

Embodiment 2

[0047] Microfluidic chip making

[0048]The porous filter is taken at the position of the top-level chip corresponding to the window, and the porous filter film (2) is placed on the glass sheet for 1 hour, then, for 30 minutes, the porous filter film (2) is treated with silylation. After the top chip performs an irreversible oxygen oriented ion, it is placed in an 80 degree oven for 30 minutes. A PDMS polymer having a monomer and an initiator ratio of 20: 1, 10 um-50 um thick film on the glass sheet, the upper surface of the underlayer chip is treated with oxygen plasma, dip PDMS, and the top layer The porous filtration of the chip is aligned, placed in an 80 degree oven, is heated, and 30 minutes is solidified. After taking the sealed chip from the oven, cut into the required size.

Embodiment 3

[0050] Cell vaccination and induction

[0051] The chip was placed in a petri dish, ultraviolet sterilization 2H, and the cell culture medium was added from the chip inlet with a pipette to allow the medium to full cell culture chamber. Put it into the vacuum pump to remove empty, remove the bubbles in the cell culture chamber. The HIPS cells in the six-hole plate were digest with Reservant, and the cells were removed from the wall. After digestion, the cell suspension in the culture flask was centrifuged, and after centrifugation, dried away from the supernatant. The new medium resuscited cell resuscitation with a pipette, adding from the underlying liquid inlet of the chip, from the liquid outlet, the corresponding volume of medium, adding the medium to the culture dish, reducing the culture in the chip Volatilization of the base. The culture dish was placed in an incubator at 37 ° C for 24 h to form the cells to form a pyrogenic nodule, and then the directional differentiation ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

A method for preparing a multi-functional microfluidic chip that forms embryoid bodies in situ: take a porous filter membrane and punch holes at the position of the corresponding observation window of the top chip; place the porous filter membrane on a glass slide for ultraviolet activation and use silane chemical treatment; the porous filter membrane and the top chip are irreversibly sealed with oxygen plasma, and then heated in an oven; the polydimethylsiloxane bonding method is: throw polydimethylsiloxane film on a glass sheet, After the upper surface of the bottom chip is treated with oxygen plasma, the polydimethylsiloxane polymer film is dipped, aligned and sealed with the porous filter membrane sealed with the top chip, and heated in an oven until it is completely cured. . The microfluidic chip prepared by the present invention has the function of cell migration and invasion experiment chamber and microfluidic chip at the same time, the bottom chip can directly form embryoid body, and carry out directional differentiation to the myocardium or brain, and can be used for Evaluation of Drugs.

Description

Technical field [0001] The present invention relates to the structural design and preparation of microfluidic chips, in particular, in particular, a method of preparing a multi-functional microfluidic chip in situ forming a pyrocarbon. Background technique [0002] In the prior art, the microfluidic chip used in cell migration and invasion experiments, TRANSWELL, the traditional method is to put the Transwell chamber into the culture plate, and the interior is called the room, and the culture plate is called the room, The upper culture solution is contained in the room, and the lower culture liquid is contained in the lower chamber, and the upper and lower tissue is separated by a polycarbonate film. We use the cell species in the upper chamber, since the polycarbonate film has permeability, the ingredient in the lower culture solution can affect the cells in the upper chamber, so that the ingredients in the lower culture solution can be investigated to cell growth, exercise, etc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C09J5/02
CPCC09J5/02C12M23/16
Inventor 秦建华尹方超王丽朱玉娟
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com