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Extract of total flavonoids of cyperus esculentus, active monomer orientin and extraction method and application thereof

A technology of active monomers and sedges, which can be applied in drug combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as research reports on the intervention effect of orientin that have not yet been seen.

Inactive Publication Date: 2018-06-15
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above studies all focus on the use of cell models and animal models to study the effect and mechanism of orientin on myocardial ischemia, but there is no research report on the intervention effect of orientin on ischemic stroke

Method used

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  • Extract of total flavonoids of cyperus esculentus, active monomer orientin and extraction method and application thereof
  • Extract of total flavonoids of cyperus esculentus, active monomer orientin and extraction method and application thereof
  • Extract of total flavonoids of cyperus esculentus, active monomer orientin and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: The total flavonoid extract of Cyperus oleifera is obtained according to the following preparation method: in the first step, the stems and leaves of Cyperus oleifera after drying are reflux extracted with 50% to 95% ethanol solution for 2 to 3 Second, the extracts are combined, and the combined extracts are concentrated under reduced pressure to obtain medicinal extracts; in the second step, the extracts obtained in the second step are separated by macroporous resin column chromatography after pretreatment, during the separation process , followed by elution with water and 30% ethanol solution to obtain 30% ethanol eluted parts; the third step, the 30% ethanol eluted parts were sequentially concentrated under reduced pressure and freeze-dried to obtain the total flavonoids extract of Cyperus oleifera .

[0052] According to the method described in this example, 100g of Cyperus oleifera can obtain 0.82g of total flavonoids extract of Cyperus oleifera.

[...

Embodiment 2

[0055] Embodiment 2: The total flavonoid extract of Cyperus oleifera is obtained according to the following preparation method: in the first step, the dried stems and leaves of Cyperus oleifera are refluxed and extracted with 50% or 95% ethanol solution for 2 times or 3 times. Second, the extracts are combined, and the combined extracts are concentrated under reduced pressure to obtain medicinal extracts; in the second step, the extracts obtained in the second step are separated by macroporous resin column chromatography after pretreatment, during the separation process , followed by elution with water and 30% ethanol solution to obtain 30% ethanol eluted parts; the third step, the 30% ethanol eluted parts were sequentially concentrated under reduced pressure and freeze-dried to obtain the total flavonoids extract of Cyperus oleifera .

Embodiment 3

[0056] Embodiment 3: As the optimization of the above embodiment, in the first step, the time of each extraction is 1.5h; or / and, the macroporous resin adopts D101 macroporous resin, and the pretreatment of D101 macroporous resin is carried out as follows : The macroporous resin is refluxed with absolute ethanol until the macroporous resin is fully swollen, and then gradiently eluted with different concentrations of absolute ethanol until the eluent is added with water without white turbid dissolution, and then eluted with distilled water. Until the eluted solution has no alcohol smell.

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Abstract

The invention relates to the technical field of extraction and application of total flavonoids of plants and relates to extract of total flavonoids of cyperus esculentus, an active monomer orientin and an extraction method and application thereof. The preparation method of the extract of total flavonoids of cyperus esculentus comprises carrying out reflux extraction on aerial stems and leaves of cyperus esculentus through an ethanol solution, mixing the extract, carrying out reduced pressure concentration to obtain extract, carrying out macroporous resin column chromatography on the pretreatedextract, carrying out elution in separation orderly through water and an ethanol solution with the content of 30% to obtain eluate of the ethanol solution with the content of 30%, and orderly carrying out reduced pressure concentration and freeze drying through the eluate of the ethanol solution with the content of 30% to obtain the extract of total flavonoids of cyperus esculentus. The inventiondiscloses the application of the extract of total flavonoids of cyperus esculentus and the active monomer orientin in preparation of drugs for treating ischemic stroke. The pharmacological experimentresult shows that the extract of total flavonoids of cyperus esculentus and the active monomer orientin can protect I / R injury.

Description

technical field [0001] The present invention relates to the technical field of extraction and application of total plant flavonoids, and relates to an extract of total flavonoids from Cyperus oleifera and a preparation method thereof, the application of the total flavonoids extract from Cyperus oleifera in the preparation of medicines for treating ischemic cerebral apoplexy, and Active monomer orientin and its extraction method, the application of the active monomer orientin in the preparation of drugs for the treatment of ischemic stroke, the active monomer orientin in the preparation of the treatment of ischemia reperfusion 24 hours caused Application of ischemic stroke drugs. Background technique [0002] Cerebral apoplexy, also known as cerebrovascular accident or stroke, is a serious cerebrovascular disease. Stroke has the characteristics of high morbidity, high mortality, high disability rate, high recurrence rate and many complications. It is listed as the three majo...

Claims

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Application Information

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IPC IPC(8): A61K36/8905A61P9/10C07D407/04
CPCA61K36/8905A61K2236/333A61K2236/39A61K2236/51A61K2236/55C07D407/04
Inventor 敬思群陈婧萱
Owner XINJIANG UNIVERSITY
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