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A magnetic graphene oxide immobilized enzyme and its application in wet corn soaking

A technology for immobilizing enzymes and oxidized stones, applied to biochemical equipment and methods, enzymes, etc. fixed on or in inorganic carriers, can solve the problems of reducing economic benefits, environmental pollution, corrosion of equipment and pipelines, etc., and achieve protein The effect of low content, high yield and low dosage

Active Publication Date: 2021-03-05
山东科桥生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sulfur dioxide produced in the traditional immersion process is the main component of acid rain, which will cause environmental pollution. At the same time, sulfurous acid will also corrode equipment and pipelines, increasing production costs.
In the final product, the residue of sulfur dioxide will affect the quality of starch products and the utilization of by-products, reducing economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Take 10g of graphene oxide (GO) and add 600mL of ethanol solution with a volume fraction of 30%, after 30KHz ultrasonic dispersion for 0.5h, add ferrous chloride (15mmol) and ferric chloride (30mmol) under stirring, and continue stirring After 30 minutes, react at 50°C for 2 hours, add ammonia water to adjust the pH to 11, and continue stirring for 1 hour, then magnetically separate, wash with water, and dry to obtain GO / Fe 3 o 4 Composite magnetic materials;

[0019] (2) Take the GO / Fe obtained in step (1) 3 o 4 Composite magnetic material (1.0g) and papain (50mg) were placed in 80mL phosphate buffer solution pH 6.0, and after 30KHz ultrasonication for 45min, the magnetic graphene oxide immobilized enzyme (hereinafter referred to as product) was obtained by magnetic separation, washing and drying. A, the enzyme immobilization rate is 82.1%).

Embodiment 2

[0021] (1) Take 10g of graphene oxide (GO) and add 400mL of ethanol solution with a volume fraction of 50%. After 40KHz ultrasonic dispersion for 0.5h, add ferrous sulfate (25mmol) and ferric sulfate (50mmol) under stirring, and continue stirring for 30 minutes Finally, react at reflux temperature for 1 hour, add ammonia water to adjust the pH to 11, continue to stir for 2 hours, and obtain GO / Fe by magnetic separation, washing and drying. 3 o 4 Composite magnetic materials;

[0022] (2) Take the GO / Fe obtained in step (1) 3 o 4 Composite magnetic material (1.0g) and papain (80mg) were placed in 100mL phosphate buffer solution pH 7.0, and after 40KHz ultrasonication for 30min, the magnetic graphene oxide immobilized enzyme (hereinafter referred to as product) was obtained by magnetic separation, washing and drying. B, the immobilization rate of the enzyme is 80.5%).

Embodiment 3

[0024] Commercially available degerminated corn (100kg) was crushed to 20-40 mesh, water (300kg) and product A (100g) were added, and after stirring and soaking at 50°C for 6 hours, the magnetic graphene oxide immobilized enzyme was reclaimed by magnetic separation ( After the recovery rate is 99.2%), the remaining soaked mixture is transferred to a pulverizer for fine pulverization, and the filtrate is allowed to stand overnight after crossing a 200 mesh sieve, centrifuged, and dried to obtain 71.3kg of cornstarch (protein≤0.4%).

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PUM

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Abstract

The invention relates to a magnetic graphene oxide immobilized enzyme and its application in wet corn soaking. The magnetic graphene oxide immobilized enzyme can rapidly separate corn starch and protein, with simple process steps and high yield of corn starch. High, low protein content, and after the magnetic graphene oxide immobilized enzyme is recycled 10 times, the corn starch yield and product quality are basically unchanged.

Description

technical field [0001] The invention belongs to the field of corn starch extraction, in particular to a magnetic graphene oxide immobilized enzyme and its application in wet corn soaking. Background technique [0002] The traditional corn starch extraction process uses old pulp and new acid (about 0.2% sulfurous acid solution) to soak in a soaking tank. The complete soaking process includes adding old pulp to new tanks, adding new acid to corn that has been soaked for a long time There is a regular cycle from tank to tank. This circulation process can maintain a certain concentration difference between the corn and the soluble substances in the soaking liquid, and soak the soluble substances in the corn. However, the sulfur dioxide produced in the traditional immersion process is the main component of acid rain, which will cause environmental pollution. At the same time, sulfurous acid will corrode equipment and pipelines and increase production costs. In the final product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C08B30/04
CPCC08B30/044C12N9/63C12N11/14C12Y304/22002
Inventor 刘庆艾史建国马耀宏杨俊慧孟庆军王丙莲马恒李秋顺公维丽蔡雷郑岚高广恒
Owner 山东科桥生物技术有限公司