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A method for the detection of sulfhydryl biomolecules based on dual-emission fluorescence detection of doped ZnS nanocrystal probes

A dual emission fluorescence, biomolecular technology, applied in the field of chemical measurement, can solve the problem of being easily interfered by external environmental factors, and achieve the effect of convenient and fast method, simple operation, and avoidance of interference.

Active Publication Date: 2020-12-25
ANHUI UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this single luminous intensity change is easily disturbed by external environmental factors, and the use of dual-emission fluorescence properties of doped nanocrystals to detect biomolecules has not been reported in the literature.

Method used

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  • A method for the detection of sulfhydryl biomolecules based on dual-emission fluorescence detection of doped ZnS nanocrystal probes
  • A method for the detection of sulfhydryl biomolecules based on dual-emission fluorescence detection of doped ZnS nanocrystal probes
  • A method for the detection of sulfhydryl biomolecules based on dual-emission fluorescence detection of doped ZnS nanocrystal probes

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Experimental program
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Effect test

Embodiment 1

[0026] 1) Preparation of Cu-doped ZnS nanocrystals

[0027] Disperse 878 mg of zinc acetate dihydrate, 10 mg of copper sulfate pentahydrate and 87 μL of mercaptopropionic acid in 200 mL of ultrapure water to form a solution, and stir evenly. Then the sodium hydroxide solution adjusted the pH value of the solution to 9 to obtain the precursor solution. A microwave reaction tube was used to take 15 mL of the precursor solution and place it in a microwave reactor, set the reaction temperature to 120°C, and stop the experiment after the reaction was carried out for about 50 minutes, and the Cu-doped ZnS nanocrystal solution was obtained. Cu-doped ZnS nanocrystals with dual emission bands can be obtained by centrifugal washing, the appearance is shown in figure 1 , keep away from light for later use.

[0028] 2) Modulation of double emission fluorescence of doped nanocrystals

[0029] Take an appropriate amount of Cu-doped ZnS nanocrystals and disperse them in water, then add bi...

Embodiment 2

[0033] 1) Preparation of Co-doped ZnS nanocrystals

[0034] Disperse 878 mg of zinc acetate dihydrate, 12 mg of cobalt sulfate pentahydrate and 87 μL of mercaptopropionic acid in 180 mL of ultrapure water to form a solution, and stir evenly. Then the sodium hydroxide solution adjusted the pH value of the solution to 9 to obtain the precursor solution. Take 20mL of the precursor solution in a microwave reaction tube and place it in a microwave reactor, set the reaction temperature at 120°C, and stop the experiment after the reaction is carried out for about 60 minutes, and the Co-doped ZnS nanocrystal solution is obtained. Co-doped ZnS nanocrystals with double emission bands can be obtained by centrifuging and washing, and stored in the dark for future use.

[0035] 2) Modulation of double emission fluorescence of doped nanocrystals

[0036] An appropriate amount of Co-doped ZnS nanocrystals was dispersed in water, and then bicyclohexanone oxalyldihydrazone was added successi...

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Abstract

The invention discloses a method for detecting mercapto boimolecules based on dually-emitted fluorescence of a zinc sulfide-doped nano crystal probe, which belongs to the technical field of chemical measurement. The method comprises the following steps: preparing zinc sulfide-doped nano crystals, modulating a dually-emitted fluorescence signal, establishing a standard curve, and detecting the mercapto biomolecules, wherein the standard curve is a curve of a correspondence between the variation of a dually-emitted fluorescent signal intensity specific value determined under the excitation of ultraviolet rays and a concentration of a substance to be detected, i.e. the standard curve which is established by successively adding a sample solution of the substance to be detected with a gradientconcentration into a zinc sulfide-doped nano crystal probe modulated via the spectrum with concentration being 1 to 100 microgrammes per milliliter under the excitationof the ultraviolet rays and gradually determining a specific value of the fluorescence intensity. By adopting the method, the interference of other impurities in the sample can be effectively avoided, the selectivity is good, a pretreatment process is omitted, and the sensitive detection for the mercapto biological active molecules such as glutathione, cysteine, homocysteine can be achieved.

Description

Technical field: [0001] The invention belongs to the technical field of chemical measurement, and relates to an analysis method of mercapto biomolecules, in particular to a fluorescence analysis and detection method of a trace amount of mercapto biomolecules, in particular to a dual-sensor method based on doped zinc sulfide nanocrystal probes. A method for detecting sulfhydryl biomolecules by emitting fluorescence. Background technique: [0002] Many small active sulfhydryl molecules (including glutathione, cysteine, homocysteine, etc.) It plays a key role and is also an important marker molecule directly related to many diseases and cancers. Therefore, low-cost identification and detection of these sulfhydryl biomolecules is very important for disease diagnosis and medical research, and has become a research hotspot in the fields of chemistry, medicine and life sciences. At present, the methods for detecting these trace active molecules mainly include enzyme-linked immuno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 何利芳张奎
Owner ANHUI UNIVERSITY OF TECHNOLOGY
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