A method for the detection of sulfhydryl biomolecules based on dual-emission fluorescence detection of doped ZnS nanocrystal probes

Abstract

The invention discloses a method for detecting mercapto boimolecules based on dually-emitted fluorescence of a zinc sulfide-doped nano crystal probe, which belongs to the technical field of chemical measurement. The method comprises the following steps: preparing zinc sulfide-doped nano crystals, modulating a dually-emitted fluorescence signal, establishing a standard curve, and detecting the mercapto biomolecules, wherein the standard curve is a curve of a correspondence between the variation of a dually-emitted fluorescent signal intensity specific value determined under the excitation of ultraviolet rays and a concentration of a substance to be detected, i.e. the standard curve which is established by successively adding a sample solution of the substance to be detected with a gradientconcentration into a zinc sulfide-doped nano crystal probe modulated via the spectrum with concentration being 1 to 100 microgrammes per milliliter under the excitationof the ultraviolet rays and gradually determining a specific value of the fluorescence intensity. By adopting the method, the interference of other impurities in the sample can be effectively avoided, the selectivity is good, a pretreatment process is omitted, and the sensitive detection for the mercapto biological active molecules such as glutathione, cysteine, homocysteine can be achieved.

Description

Technical field:

[0001] The invention belongs to the technical field of chemical measurement, and relates to an analysis method of mercapto biomolecules, in particular to a fluorescence analysis and detection method of a trace amount of mercapto biomolecules, in particular to a dual-sensor method based on doped zinc sulfide nanocrystal probes. A method for detecting sulfhydryl biomolecules by emitting fluorescence. Background technique:

[0002] Many small active sulfhydryl molecules (including glutathione, cysteine, homocysteine, etc.) It plays a key role and is also an important marker molecule directly related to many diseases and cancers. Therefore, low-cost identification and detection of these sulfhydryl biomolecules is very important for disease diagnosis and medical research, and has become a research hotspot in the fields of chemistry, medicine and life sciences. At present, the methods for detecting these trace active molecules mainly include enzyme-linked immuno...

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