Flexible laccase electrode responsive to dual stimulation of temperature and pH and its construction method

A dual stimulus-response and stimulus-response technology, applied in battery electrodes, circuits, structural parts, etc., can solve the problems of low enzyme load, reduced electron transfer efficiency to electrodes, poor stability, etc., achieving good catalytic performance and satisfying load stability. Good performance and stability

Inactive Publication Date: 2020-05-19
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the "on / off" functional enzyme biofuel cell currently studied is only a conceptual battery
On the one hand, because the current enzyme electrode itself has low enzyme loading, low electron transfer efficiency, and easy enzyme detachment, the enzyme fuel cell constructed based on this inevitably has application bottlenecks such as low open circuit voltage, low power density, and poor stability.
On the other hand, the electrochemical responsiveness of responsive enzyme electrodes generally depends on the stimuli-responsive polymer on the surface of the electrode. The worse the electrical performance, the lower the efficiency of electron transfer to the electrode; in addition, the stimuli-responsive polymer brush The modification method on the electrode is mostly drop coating, the interaction between the prepared response layer and the electrode is small, and it is easy to fall off during use, resulting in serious attenuation of the "on / off" function of the electrode, and "on / off" less effective

Method used

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  • Flexible laccase electrode responsive to dual stimulation of temperature and pH and its construction method
  • Flexible laccase electrode responsive to dual stimulation of temperature and pH and its construction method
  • Flexible laccase electrode responsive to dual stimulation of temperature and pH and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Implementation example 1: the reaction flow diagram is as figure 1 shown.

[0023] a. Pretreatment of foam nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically in acetone, 0.5mM sulfuric acid, ultrapure water, and in 0.1mM NiCl 4 Soak in the water solution for 4 hours, then take it out and dry it naturally;

[0024] b. Preparation of nano-gold deposition solution: 40 μL of 10 mg / mL chloroauric acid solution was added to 10 ml of K with a pH value of 8 2 HPO 4 -KH 2 PO 4 In the buffer solution, stir until completely dissolved and feed nitrogen to saturation to obtain nano-gold deposition solution;

[0025] c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scanning in the nano-gold deposit...

Embodiment 2

[0029] Implementation example 2: the reaction flow diagram is as figure 1 shown.

[0030] a. Pretreatment of foam nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically in acetone, 0.5mM sulfuric acid, ultrapure water, and in 0.1mM NiCl 4 Soak in the water solution for 4 hours, then take it out and dry it naturally;

[0031] b. Preparation of nano-gold deposition solution: Add 40 μL of 10 mg / mL chloroauric acid solution to 10 ml of K with a pH value of 8 2 HPO 4 -KH 2 PO 4 In the buffer solution, stir until completely dissolved and feed nitrogen to saturation to obtain nano-gold deposition solution;

[0032] c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scanning in the nano-gold deposition solu...

Embodiment 3

[0036] Implementation example 3: the reaction flow diagram is as figure 1 shown.

[0037] a. Pretreatment of foam nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically in acetone, 0.5mM sulfuric acid, ultrapure water, and in 0.1mM NiCl 4 Soak in the water solution for 4 hours, then take it out and dry it naturally;

[0038] b. Preparation of nano-gold deposition solution: Add 40 μL of 10 mg / mL chloroauric acid solution to 10 ml of K with a pH value of 8 2 HPO 4 -KH 2 PO 4 In the buffer solution, stir until completely dissolved and feed nitrogen to saturation to obtain nano-gold deposition solution;

[0039] c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scanning in the nano-gold deposition so...

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Abstract

The invention relates to a temperature and pH value dual-stimulation responsive flexible laccase electrode and a construction method. According to the method, the dual-stimulation responsive laccase electrode is prepared on a flexible foam nickel conductive substrate through free radical electro-copolymerization, and laccase is fixed in a covalent bond manner, so that the electrode stability can be better met. One temperature-stimulation responsive monomer and two pH-stimulation responsive monomers are selected and subjected to free radical electro-copolymerization on the surface of the laccase electrode, and the prepared stimulation responsive laccase electrode has good on / off performance, has the catalytic peak current of 0.000771 A in a 20 mM glucose solution and has good catalytic performance. The electrode has the catalytic peak current only decreased by 6.49% in 10 times of on / off cycles and has good stability. The electrode can meet the cathode use requirement in EBFCs (enzymatic biofuel cells).

Description

technical field [0001] The invention belongs to the technical field of preparation of flexible stimuli-responsive enzyme electrodes, and relates to a flexible laccase electrode with double stimuli responses to temperature and pH value and a construction method thereof, and to a polymer with double stimuli responses to temperature and pH value prepared by electro-induced free radical copolymerization Membrane Modified Enzyme Electrode Method. Background technique [0002] Enzyme bioelectrodes are electrodes constructed with biologically active enzymes instead of noble metal catalysts. The electrode can catalyze the oxidation-reduction reaction of the substrate in the physiological body fluid environment, and convert the gain and loss of electrons into electrical signal output. The battery constructed with this electrode is the enzyme biofuel cell (EBFC), which is currently the most promising green and renewable micro-fuel cell that can be used for implanting small medical de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01M4/86H01M4/88H01M8/16
CPCH01M4/8605H01M4/8825H01M8/16Y02E60/50
Inventor 马晓燕陈影索鸿飞屈枫锦
Owner NORTHWESTERN POLYTECHNICAL UNIV
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