A flexible glucose oxidase electrode in response to pH stimulation and its construction method

A glucose oxidase and stimuli-response technology, applied in the field of preparation of flexible stimuli-responsive enzyme electrodes, can solve the problems of reduced electron transfer efficiency, low power density, small interaction between the response layer and the electrode, and achieve good on/off performance , Satisfy the effect of electrode stability and good cycle stability

Inactive Publication Date: 2020-07-14
RES & DEV INST OF NORTHWESTERN POLYTECHNICAL UNIV IN SHENZHEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the "on / off" functional enzyme biofuel cell currently studied is only a conceptual battery
On the one hand, because the current enzyme electrode itself has low enzyme loading, low electron transfer efficiency, and easy enzyme detachment, the enzyme fuel cell constructed based on this inevitably has application bottlenecks such as low open circuit voltage, low power density, and poor stability.
On the other hand, the electrochemical responsiveness of responsive enzyme electrodes is generally achieved by the presence of stimuli-responsive polymers on the electrode surface, which reduces the electron transfer efficiency. The response layer prepared in this way has little interaction with the electrode, and it is easy to fall off during use, resulting in serious attenuation of the "on / off" function of the electrode and poor "on / off" effect

Method used

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  • A flexible glucose oxidase electrode in response to pH stimulation and its construction method
  • A flexible glucose oxidase electrode in response to pH stimulation and its construction method
  • A flexible glucose oxidase electrode in response to pH stimulation and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Implementation example 1: the reaction flow diagram is as figure 1 shown.

[0028] a. Pretreatment of foamed nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically clean it in dilute sulfuric acid, acetone, and ultrapure water, and wash it in 0.1mM NiCl 4 Soak in the water solution for 3 hours, then take it out and let it dry naturally;

[0029] b. Preparation of nano-gold deposition solution: 50 μL of chloroauric acid solution of 10 mg / mL was added to 10 ml of Na with a pH value of 8 2 HPO 4 -NaH 2 PO 4 In the buffer solution, feed nitrogen gas to saturation to obtain a gold nanometer deposition solution;

[0030]c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scan in the nano-gold depo...

Embodiment 2

[0036] Implementation example 2: the reaction flow diagram is as figure 1 shown.

[0037] a. Pretreatment of foamed nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically clean it in dilute sulfuric acid, acetone, and ultrapure water, and wash it in 0.3mM NiCl 4 Soak in the water solution for 4 hours, then take it out and dry it naturally;

[0038] b. Preparation of nano-gold deposition solution: 60 μL of 10 mg / mL chloroauric acid solution was added to 10 ml of Na with a pH value of 8 2 HPO 4 -NaH 2 PO 4 In the buffer solution, feed nitrogen gas to saturation to obtain a gold nanometer deposition solution;

[0039] c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scan in the nano-gold deposition...

Embodiment 3

[0045] Implementation example 3: the reaction flow diagram is as figure 1 shown.

[0046] a. Pretreatment of foamed nickel electrode: Cut the electrode-grade nickel foam into an appropriate size, and ultrasonically clean it in dilute sulfuric acid, acetone, and ultrapure water, and wash it in 0.5mM NiCl 4 Soak in the water solution for 6 hours, then take it out and dry it naturally;

[0047] b. Preparation of nano-gold deposition solution: 70 μL of 10 mg / mL chloroauric acid solution was added to 10 ml of Na with a pH value of 8 2 HPO 4 -NaH 2 PO 4 In the buffer solution, feed nitrogen gas to saturation to obtain a gold nanometer deposition solution;

[0048] c. Preparation of nano-gold hybrid nickel foam electrode: take the electrode processed in step a as the working electrode, the platinum column electrode as the counter electrode, and the silver / silver chloride electrode as the reference electrode to construct a three-electrode system. Scan in the nano-gold deposition...

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PUM

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Abstract

The invention relates to a pH value stimulus-response flexible glucose oxidase electrode and a construction method. The construction method comprises the steps of bonding and fixing, via a covalent bond, a glucose oxidase on a surface of a foamed nickel electrode deposited with nanogold particles, then modifying an SI-eATRP initiator on the electrode via an amide reaction, and then polymerizing apH-sensitive monomer via the SI-eATRP to obtain the flexible glucose oxidase electrode with pH value stimulus response and excellent cyclic stability. According to the pH value stimulus-response flexible glucose oxidase electrode and the construction method provided by the invention, by adopting the method of fixing an enzyme with the covalent bond, the stability of the electrode can be well met;by modifying an amino of a mercapto coupling agent and an amino carried by a protein shell of the enzyme into the SI-eATRP initiator via the amide reaction, selecting the pH stimulus-response monomerand copolymerizing via the SI-eATRP on the surface of the enzyme electrode, the prepared stimulus-response enzyme electrode has good "on / off" performance and excellent cyclic stability; and in five times of "on / off" circulation of the electrode, the catalytic peak current is reduced by 18.6%, and the stability is good.

Description

technical field [0001] The invention belongs to the technical field of preparation of flexible stimuli-responsive enzyme electrodes, and relates to a pH-value stimuli-responsive flexible glucose oxidase electrode and a construction method, in particular to a pH-value stimuli-responsive polymer prepared by electrically mediated surface-triggered atom transfer radical polymerization Method for brush modification of enzyme electrodes. Background technique [0002] Enzyme bioelectrodes are electrodes constructed with biologically active enzymes instead of noble metal catalysts. The electrode can catalyze the oxidation-reduction reaction of the substrate in the physiological body fluid environment, and convert the gain and loss of electrons into electrical signal output. The battery constructed with this electrode is the enzyme biofuel cell (EBFC), which is currently the most promising green and renewable micro-fuel cell that can be used for implanting small medical devices in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 马晓燕索鸿飞陈影屈枫锦
Owner RES & DEV INST OF NORTHWESTERN POLYTECHNICAL UNIV IN SHENZHEN
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