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A Bifidobacterium lactis strain, Bifidobacterium lactis freeze-dried powder and a preparing method thereof

A technology of Bifidobacterium lactis and freeze-dried powder is applied in the field of Bifidobacterium lactis strains, Bifidobacterium lactis freeze-dried powder and preparation thereof, and can solve the problem of low number of live bacteria, high cost, and survival rate of storage at room temperature in freeze-dried powder. Low problems, to prevent oxidative damage, prevent interactions, and ensure the number of viable bacteria

Active Publication Date: 2018-06-29
金华银河生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present application is to provide a strain of Bifidobacterium lactis, and the second purpose of the present application is to provide a method for preparing the Bifidobacterium lactis into freeze-dried powder to solve the high cost of the existing freeze-drying method, Moreover, the number of viable bacteria in the freeze-dried powder is low and the survival rate of normal temperature storage is low.

Method used

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  • A Bifidobacterium lactis strain, Bifidobacterium lactis freeze-dried powder and a preparing method thereof
  • A Bifidobacterium lactis strain, Bifidobacterium lactis freeze-dried powder and a preparing method thereof
  • A Bifidobacterium lactis strain, Bifidobacterium lactis freeze-dried powder and a preparing method thereof

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Embodiment 1

[0106] 1. Prefabrication of embedding protectant

[0107] ①Preparate 0.01kg of transglutaminase to make 10% enzyme solution, filter and sterilize the enzyme solution through a 0.22μm sterile filter membrane, and refrigerate for later use;

[0108] ② Prepare 50% solution of 0.05kg calcium chloride and sterilize at 121°C for 15 minutes for later use;

[0109] ③Prepare 0.05kg of cysteine ​​hydrochloride into a 50% solution and sterilize at 121°C for 15 minutes;

[0110] ④ Prepare 1kg of skimmed milk powder into a 15% solution and sterilize at 121°C for 7 minutes for later use;

[0111] ⑤ Put 0.02kg of chitosan into a 5% solution and sterilize at 121°C for 15 minutes for later use;

[0112] ⑥ Prepare 0.02kg of gelatin into a 5% solution and sterilize at 121°C for 15 minutes for later use;

[0113] ⑦ Prepare 1kg trehalose into a 50% solution and sterilize at 121°C for 15 minutes for later use;

[0114] ⑧ Prepare 5% solution of 0.02kg sodium alginate and sterilize at 121°C for 1...

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Abstract

The invention provides a Bifidobacterium lactis strain (that is Bifidobacterium lactis BL9) with an accession number of CGMCC No.14536. The invention also provides a preparing method of Bifidobacterium lactis freeze-dried powder. The method includes performing embedding and freeze-drying treatment in an anaerobic environment, and before freeze-drying treatment, a plurality of antioxidants are adopted to perform embedding treatment on the Bifidobacterium lactis in steps, thus effectively preventing interactions between oxygen and a cytomembrane system of the Bifidobacterium lactis, preventing behaviors damaging DNA synthesis, and clearing free radicals generated before freeze drying of the strain, and therefore oxidation damage in a drying process is prevented, and the Bifidobacterium lactis is not liable to be deactivated under room-temperature storage conditions. The invention also provides Bifidobacterium lactis freeze-dried powder prepared by the method.

Description

technical field [0001] The application relates to the field of biotechnology, in particular to a strain of Bifidobacterium lactis, freeze-dried powder of Bifidobacterium lactis prepared by using the Bifidobacterium lactis and a preparation method thereof. Background technique [0002] Bifidobacterium lactis is a beneficial bacterium that colonizes the human intestinal tract, and is the dominant flora in the intestinal tract of healthy people. The structural composition of Bifidobacterium lactis in the human intestinal flora is related to the age and sex of the host, as well as the diet structure and lifestyle of the host. The anaerobic bacteria Bifidobacterium lactis in the intestinal tract of breastfed infants accounts for more than 92% of the total intestinal flora. It is generally believed that Bifidobacterium lactis is a strictly anaerobic bacterium, which has physiological functions such as regulating intestinal flora, preventing and treating intestinal-related disease...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/20C12N1/205C12R2001/01
Inventor 张建军姚国强
Owner 金华银河生物科技有限公司
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