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A strain of Acinetobacter oilophilus nmb17 and its application in plant disease control

A bacillus and plant technology, applied in applications, animal repellents, plant growth regulators, etc., can solve problems such as unsatisfactory control effects, pathogenic bacteria resistance to human health, and hazards

Active Publication Date: 2019-12-31
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of plant soil-borne diseases mainly use disease-resistant varieties, agricultural measures and the use of chemical agents, etc., but the control effects are not ideal, especially the use of chemical pesticides, which not only makes pathogenic bacteria develop drug resistance but also has serious consequences for human health. harm

Method used

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  • A strain of Acinetobacter oilophilus nmb17 and its application in plant disease control
  • A strain of Acinetobacter oilophilus nmb17 and its application in plant disease control
  • A strain of Acinetobacter oilophilus nmb17 and its application in plant disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, isolation and identification of NMB17 bacterial strain

[0078] 1. Isolation of NMB17 strain

[0079] The NMB17 strain was isolated from the rhizosphere soil where the sugar beet root rot was severe in the sugar beet continuous cropping plot in Ulanqab City, Inner Mongolia Autonomous Region. The specific separation method is as follows: pick the rhizosphere soil collected from the sugar beet continuous cropping plot, pick the root system, scrape the rhizosphere soil, weigh 5g, put it into the prepared sterilized triangular flask under aseptic conditions, and wash it with sterilized water. The gradient was diluted to 10-6 times, and 3 replicates were set. Take 200 μL of the soil samples that were diluted in gradient and spread them on the PDA medium plate respectively, cultivate them in a 28°C incubator, and observe the growth status of the colonies every day. Basically no colony grows on the plate, and when the dilution is 10-4 times, a single colony can...

Embodiment 2

[0096] Embodiment 2, the preparation of microbial NMB17 bacterial agent, fermented liquid and metabolic liquid

[0097] 1. Preparation of microbial NMB17 bacterial agent

[0098] Inoculate the Acinetobacter oleophilus NMB17 obtained in Example 1 into LB medium, shake and culture at 28°C and 200rpm for 12-16h, then centrifuge at 4000rpm for 20min, discard the supernatant, collect the bacterial precipitate, and use Sterilized water is used to dilute the bacterial precipitate to make microbial NMB17 bacterial agent, and the total concentration of NMB17 viable bacteria in the finished product of microbial NMB17 bacterial agent is 1×10 9 -1×10 10 CFU / mL.

[0099] 2. Preparation of microbial NMB17 fermentation broth

[0100] The Acinetobacter oleophilus NMB17 obtained in Example 1 was inoculated in LB culture medium, shaken and cultured at 28°C and 200rpm for 12-16h, then centrifuged at 4000rpm for 20min, and the supernatant was collected, which was the microbial NMB17 fermentati...

Embodiment 3

[0103] Embodiment 3, the influence of microorganism NMB17 fermented liquid and bacterial agent on the antibacterial rate of cotton Verticillium dahliae V991

[0104] 1. Experimental method

[0105] 1. Add the NMB17 fermentation broth to the PDA medium that has been melted and cooled to about 50°C according to the volume ratio of 1:25, mix well and spread the plate to obtain the PDA medium containing the NMB17 fermentation broth. At the same time, the PDA medium without any bacterial agent was set as the control.

[0106] The PDA medium without adding any bacterial agent was spread on the plate, and the center point of the plate was drawn by the cross method, and holes were punched at 1.5cm and 2.5cm away from the center point respectively, and then the diameter of the hole was punched. For a 5cm round hole, add 10 μl of NMB17 bacterial agent to the round hole, and use 10 μl of sterilized water as a control, place it in an ultra-clean bench and wait for the bacteria solution a...

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Abstract

The invention discloses a strain of Acinetobacter oilophilus NMB17 and its application in preventing and treating plant diseases and storage molds. The invention provides a strain of Acinetobacter oilophilus NMB17, and prepares microbial NMB17 bacterial agent, fermented liquid and metabolic liquid. Proved by experiments: Acinetobacter oilophilus NMB17 of the present invention or its bacterium agent or its fermented liquid or its metabolism liquid or its bacterium suspension or its culture liquid or its volatile matter can broad-spectrum, efficient prevention and treatment of plant disease and storage mold, It can not only improve the resistance of plants to plant soil-borne diseases and storage molds, but also solve the problems of pesticide residues and environmental pollution, which is conducive to promoting environmental protection and sustainable agricultural development, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Acinetobacter oleophilus NMB17 and its application in plant disease control. Background technique [0002] Plant soil-borne diseases are mostly soil-inhabiting bacteria. These soil-borne diseases have the characteristics of wide host range, strong stress resistance, and difficulty in eradication. It is especially difficult to control combined infection. In production, there is often a lack of excellent disease-resistant varieties and effective chemical control agents. [0003] Plant root rot, cotton verticillium wilt, etc. are soil and seed-borne systemic diseases with a wide range of hosts. They are also typical soil-borne diseases that cause crop disease. The main pathogenic bacteria include Fusarium and Pathogenic fungi such as Verticillium dahliae Kleb. These pathogenic fungi can produce a large number of microsclerotias, survive in the soil for a long tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 叶健姚香梅龚雨晴赵启祥
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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