Acinetobacter oleivorans NMB 17, and applications thereof in plant disease prevention and treatment

A bacillus and plant technology, applied in applications, animal repellents, plant growth regulators, etc., can solve problems such as unsatisfactory control effects, pathogenic bacteria resistance to human health, and hazards

Active Publication Date: 2018-06-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of plant soil-borne diseases mainly use disease-resistant varieties, agricultural measures and the use of chemical agents, etc., but the control ef

Method used

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  • Acinetobacter oleivorans NMB 17, and applications thereof in plant disease prevention and treatment
  • Acinetobacter oleivorans NMB 17, and applications thereof in plant disease prevention and treatment
  • Acinetobacter oleivorans NMB 17, and applications thereof in plant disease prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, isolation and identification of NMB17 bacterial strain

[0078] 1. Isolation of NMB17 strain

[0079] The NMB17 strain was isolated from the rhizosphere soil where the sugar beet root rot was severe in the sugar beet continuous cropping plot in Ulanqab City, Inner Mongolia Autonomous Region. The specific separation method is as follows: pick the rhizosphere soil collected from the sugar beet continuous cropping plot, pick the root system, scrape the rhizosphere soil, weigh 5g, put it into the prepared sterilized triangular flask under aseptic conditions, and wash it with sterilized water. The gradient was diluted to 10-6 times, and 3 replicates were set. Take 200 μL of the soil samples that were diluted in gradient and spread them on the PDA medium plate respectively, cultivate them in a 28°C incubator, and observe the growth status of the colonies every day. Basically no colony grows on the plate, and when the dilution is 10-4 times, a single colony can...

Embodiment 2

[0096] Embodiment 2, the preparation of microbial NMB17 bacterial agent, fermented liquid and metabolic liquid

[0097] 1. Preparation of microbial NMB17 bacterial agent

[0098] Inoculate the Acinetobacter oleophilus NMB17 obtained in Example 1 into LB medium, shake and culture at 28°C and 200rpm for 12-16h, then centrifuge at 4000rpm for 20min, discard the supernatant, collect the bacterial precipitate, and use Sterilized water is used to dilute the bacterial precipitate to make microbial NMB17 bacterial agent, and the total concentration of NMB17 viable bacteria in the finished product of microbial NMB17 bacterial agent is 1×10 9 -1×10 10 CFU / mL.

[0099] 2. Preparation of microbial NMB17 fermentation broth

[0100] The Acinetobacter oleophilus NMB17 obtained in Example 1 was inoculated in LB culture medium, shaken and cultured at 28°C and 200rpm for 12-16h, then centrifuged at 4000rpm for 20min, and the supernatant was collected, which was the microbial NMB17 fermentati...

Embodiment 3

[0103] Embodiment 3, the influence of microorganism NMB17 fermented liquid and bacterial agent on the antibacterial rate of cotton Verticillium dahliae V991

[0104] 1. Experimental method

[0105] 1. Add the NMB17 fermentation broth to the PDA medium that has been melted and cooled to about 50°C according to the volume ratio of 1:25, mix well and spread the plate to obtain the PDA medium containing the NMB17 fermentation broth. At the same time, the PDA medium without any bacterial agent was set as the control.

[0106] The PDA medium without adding any bacterial agent was spread on the plate, and the center point of the plate was drawn by the cross method, and holes were punched at 1.5cm and 2.5cm away from the center point respectively, and then the diameter of the hole was punched. For a 5cm round hole, add 10 μl of NMB17 bacterial agent to the round hole, and use 10 μl of sterilized water as a control, place it in an ultra-clean bench and wait for the bacteria solution a...

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Abstract

The invention discloses Acinetobacter oleivorans NMB 17, and applications thereof in plant disease prevention and treatment and storage of mould. An Acinetobacter oleivorans NMB 17 bacterial preparation, a broth, or a metabolism liquid of Acinetobacter oleivorans NMB 17 are prepared. It is confirmed by experiments that Acinetobacter oleivorans NMB 17, or the bacterial preparation, or the broth, orthe metabolism liquid, or bacterial suspension, or nutrient solution, or volatile matters of Acinetobacter oleivorans NMB 17 are capable of preventing and treating plant diseases and storage of mouldwith high efficiency and wide spectrum, so that the resistance of plants on soil born diseases and storage of mould is increased, pesticide residue and environment pollution problems are solved, it is beneficial for promotion of environment protection and agriculture sustainable development, and the application prospect is promising.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Acinetobacter oleophilus NMB17 and its application in plant disease control. Background technique [0002] Plant soil-borne diseases are mostly soil-inhabiting bacteria. These soil-borne diseases have the characteristics of wide host range, strong stress resistance, and difficulty in eradication. It is especially difficult to control combined infection. In production, there is often a lack of excellent disease-resistant varieties and effective chemical control agents. [0003] Plant root rot, cotton verticillium wilt, etc. are soil and seed-borne systemic diseases with a wide range of hosts. They are also typical soil-borne diseases that cause crop disease. The main pathogenic bacteria include Fusarium and Pathogenic fungi such as Verticillium dahliae Kleb. These pathogenic fungi can produce a large number of microsclerotias, survive in the soil for a long tim...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 叶健姚香梅龚雨晴赵启祥
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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