A strain of bacillus for removing the stench of sheep manure and its application

A Bacillus, sheep manure technology, applied in bacteria, microorganism-based methods, biological sludge treatment, etc., can solve the problem of sheep feces odor and other issues

Inactive Publication Date: 2020-12-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of feces odor in sheep sheds, and provide a bacillus with high-efficiency removal ability for fecal matter of sheep manure and its application. The present invention also provides a method and microbial bacteria agent

Method used

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  • A strain of bacillus for removing the stench of sheep manure and its application
  • A strain of bacillus for removing the stench of sheep manure and its application
  • A strain of bacillus for removing the stench of sheep manure and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preliminary screening of embodiment 1 deodorizing microorganism

[0027] In fertile soil, dig up earthworms. After the surface of the earthworm is sterilized with medical alcohol, cut its cavity with dissecting scissors, take out the contents of the cavity and put it in 20mL sterile saline, stir it evenly, and after appropriate dilution, spread it on the sheep manure extract agar medium plate . Inverted at 30°C for 48 hours, single colonies with different shapes were picked, streaked on the PDA slant medium of the test tube, and named W1~W22 respectively, a total of 22 single strains.

[0028] Inoculate 22 single bacterial strains in nutrient broth medium, culture at 30°C and 100r / min shaker for 36 hours, and obtain bacterial liquid for future use. Take by weighing 50g of fresh sheep manure in a 500mL Erlenmeyer flask, and inoculate the bacterial solution by 10% of the inoculum. 3 parallels for each strain. Seal the Erlenmeyer flask with a rubber stopper, incuba...

Embodiment 2

[0029] The rescreening of embodiment 2 deodorizing microorganisms

[0030] Bacterial strains such as W7 and W15 with deodorizing effect obtained through primary screening were expanded and cultivated with nutrient broth medium, 30°C, 100r / min shaker shaking culture for 36h, and the bacterial liquid was obtained for future use. Weigh 200g of fresh sheep manure in a 1000mL Erlenmeyer flask, inoculate the bacterial solution with 10% inoculum size, and each bacterial strain has 5 parallels, while using fresh sheep manure without any treatment as a control. Seal the Erlenmeyer flask with a rubber stopper again, collect ammonia gas with 0.005N dilute sulfuric acid solution, cultivate at a constant temperature of 30°C, evaluate the deodorizing effect of each bacterial strain by sensory evaluation every 5 days, and detect the collected ammonia gas quality (NH 3 ), samples were taken to detect the content of volatile fatty acids (VAFs), indole, skatole, p-cresol and other malodorous su...

Embodiment 3

[0038] Identification of embodiment 3 bacterial strain W15

[0039] The W15 strain obtained from the above screening was identified by molecular biology methods, and the strain was sent to a sequencing company for 16S rDNA sequence determination, and the measured sequence was compared with the sequence in the GenBank database by BLAST. The similarity of Bacillus sp. is more than 99%.

[0040] 110> Huazhong Agricultural University

[0041] A strain of Bacillus for removing odor from sheep manure and its application

[0042] 1

[0043] 1

[0044] PatentIn version 3.5

[0045] 1

[0046] 1447

[0047] DNA

[0048] Bacillus sp.

[0049] 1

[0050] GGGCGGGTGC TATAATGCAA GTCGAGCGGA CAGATGGGAG CTTGCTCCCT GATGTTAGCG 60

[0051] GCGGACGGGT GAGTAACACG TGGGTAACCT GCCTGTAAGA CTGGGATAAC TCCGGGAAAC 120

[0052] CGGGGCTAAT ACCGGATGGT TGTTTGAACC GCATGGTTCA AACATAAAAG GTGGCTTCGG 180

[0053] CTACCACTTA CAGATGGACC CGCGGCGCAT TAGCTAGTTG GTGAGGTAAC GGCTCACCAA 240

[0054] GGC...

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Abstract

The invention discloses a strain of Bacillus sp. W15 for efficiently removing the stench of sheep manure, which is preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M 2017786. The present invention screens out a Bacillus strain that can significantly reduce the stench of sheep manure from the contents of the earthworm cavity, and the sensory evaluation and the detection of the content of the malodorous substance all show significant effects, which can improve the environment of the sheep house and prevent the malodorous substance from harming humans and animals. It has broad application prospects in health hazards.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus (Bacillus sp.) for removing the stench of sheep manure and its application. [0002] technical background [0003] Intensive breeding of mutton sheep in my country is increasing year by year. The large amount of feces produced by intensive farming can release a large amount of malodorous gases, including ammonia, volatile fatty acids (VFAs), indole, skatole, etc. These malodorous substances not only affect the health of animals and people in sheep houses, but also are released into the air, causing air pollution and affecting the lives of nearby residents. At present, how to efficiently and cheaply remove odorous gases and reduce their harm to animals, humans and the environment has become a major problem in the breeding industry. [0004] It has been reported that vermicompost can effectively remove malodorous substances because vermicompost contain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/07
CPCC02F11/02C02F2103/20C02F2303/02C12N1/20C12N1/205C12R2001/07
Inventor 齐德生张家才张妮娅孙铝辉於江坤汪智云
Owner HUAZHONG AGRI UNIV
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