Unlock instant, AI-driven research and patent intelligence for your innovation.

Fumonisins detection colloidal gold quick test card and kit and method for detecting fumonisins

A technology of fumonisins and colloidal gold, which is applied in measuring devices, instruments, biomaterial analysis, etc., can solve the problems of long detection time, low specificity and accuracy, and poor stability of fumonisins, and achieve portability and instant sensitivity , easy operation, high accuracy and stability

Active Publication Date: 2021-04-06
COFCO GROUP +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of long detection time, low sensitivity, specificity and accuracy, high cost and poor stability of fumonisins in the prior art, and provide a fast, high sensitivity, high specificity, Colloidal gold quick test card and kit for detecting fumonisins with high accuracy and stability and a method for detecting fumonisins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fumonisins detection colloidal gold quick test card and kit and method for detecting fumonisins

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] The inventors of the present invention have found in the course of their research that by carrying out redox reactions with a specific proportion of chloroauric acid and sodium citrate under specific conditions, and cooling the reaction product at a specific rate, uniform particle size can be obtained. Colloidal gold particles with a particle size of 10-35nm, preferably 10-30nm. When the colloidal gold particles are used to prepare a colloidal gold quick test card for the detection of mycotoxins, the sensitivity, specificity and precision of the detection can be effectively improved. Therefore, the preparation method of the colloidal gold particle in the colloidal gold solution of the present invention preferably comprises:

[0047] (i) under the condition of stirring, add sodium citrate to the boiling auric acid solution, make the auric acid and sodium citrate contact reaction 5-15min, obtain reaction product;

[0048] (ii) cooling the reaction product obtained in ste...

Embodiment 1

[0065] This embodiment is used to illustrate the preparation method of the colloidal gold quick test card provided by the present invention

[0066] (1) Preparation of colloidal gold solution

[0067]Concentration is that the chloroauric acid solution of 1.2% by weight is placed on the heating magnetic stirrer, is heated to boiling, adds 3ml trisodium citrate solution rapidly (the weight ratio of chloroauric acid and trisodium citrate is 1:3.8), the solution The color begins to change from light blue, blue, purple, and wine red in sequence. When the solution turns into wine red, continue heating for 5 minutes, then cool down to room temperature at a rate of 15°C / min, and store at 4°C for later use. Through transmission electron microscope inspection, the colloidal gold particles are uniform in size, all between 20-25nm, and form a regular spherical shape;

[0068] (2) Preparation of gold-labeled antibody

[0069] 1) Use boric acid buffer to adjust the pH value of the colloid...

Embodiment 2

[0085] This embodiment is used to illustrate the preparation method of the colloidal gold quick test card provided by the present invention

[0086] (1) Preparation of colloidal gold solution

[0087] Concentration is that the chloroauric acid solution of 1% by weight is placed on the heating magnetic stirrer, is heated to boiling, adds 2.5ml trisodium citrate solution rapidly (the weight ratio of chloroauric acid and trisodium citrate is 1:3.3), The solution began to change color, from light blue, blue, purple, and wine red in sequence. When the solution turned into wine red, continue heating for 8 minutes, then cool down to room temperature at a rate of 18 °C / min, and store at 4 °C for later use. Through the transmission electron microscope inspection, the colloidal gold particles are consistent in size, all between 15-20nm, and form a regular spherical shape;

[0088] (2) Preparation of gold-labeled antibody

[0089] 1) Use boric acid buffer to adjust the pH value of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biological detection, and discloses a colloidal gold quick test card for fumonisin detection, a kit and a method for detecting fumonisins, specifically, a colloidal gold quick test card for fumonisin detection, the The colloidal gold quick test card includes a sample loading area, a conjugate area, an observation area and a water absorption area arranged sequentially on the bottom plate; a method for detecting fumonisins is also disclosed, and the method includes: pretreatment of the sample to be tested, The fumonisin extract was obtained, and the colloidal gold quick test card was used to detect and analyze the fumonisin extract. At the same time, a kit for detecting fumonisins is disclosed, which is characterized in that the kit includes the colloidal gold quick test card, a vial filled with purified water and a vial filled with alcohol. The colloidal gold rapid test card of the present invention can rapidly and effectively improve the sensitivity, specificity and accuracy of detection.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a colloidal gold rapid detection card for detecting fumonisins, a method for detecting fumonisins and a kit for detecting fumonisins. Background technique [0002] Mycotoxins are metabolites produced by fungi growing in food or feed that are harmful to humans and animals. The earliest record of poisoning by mycotoxins is ergot poisoning in Europe in the 11th century, and the clinical symptoms of this poisoning were described in medieval icon paintings. [0003] Common mycotoxins are aflatoxin (B1), deoxynivalenol, zearalenone, fumonisins, T-2 toxin, and ochratoxin A. [0004] Fumonisins are a group of mycotoxins mainly produced by Fusarium moniliforme under certain temperature and humidity conditions. So far, 28 fumonisins analogues have been discovered, which are divided into four types: A, B, C and P. Types of. At the same time, fumonisin B1 has the strongest toxicity amo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/569
CPCG01N33/56961G01N33/577G01N2333/37
Inventor 李慧何景蔡军欧静堃胡梦龙傅洋杨学昊
Owner COFCO GROUP