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Method for improving adsorption capacity of saccharomyces cerevisiae for metal ions in food

A technology of Saccharomyces cerevisiae and metal ions, which is applied in the field of microorganisms, can solve the problems that microorganisms are not food-safe microorganisms, the types of adsorbed objects are single, and are not suitable for food production, etc., achieve important industrial application value, easy operation, and solve metal pollution problems Effect

Inactive Publication Date: 2018-07-06
ZIBO VOCATIONAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the technology of removing heavy metals by biosorption has been reported, but the microorganisms reported so far have defects such as a single type of adsorption target, microorganisms that do not belong to food safety microorganisms, and are not suitable for food production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Pick a single colony from the YPD plate and inoculate it into a 500mL Erlenmeyer flask containing 50mL seed medium, at 30°C, 220r min -1 The 24h of cultivation was used as the seed solution for fermenter cultivation. The seed solution is inoculated into a 5L fermenter containing 1.2L fermentation medium with an inoculation amount of 10% (volume ratio) and cultivated for 16-36 hours. Centrifuge at 7000rpm for 3-10min, collect the bacteria, wash the bacteria with deionized water 2-3 times,

Embodiment 2

[0033] Wash 0.5 g (dry weight) of Saccharomyces cerevisiae 3 to 4 times with 0.1 mol / L HCl, centrifuge at 3000 to 5000 rpm, and collect the bacteria. Using deionized water as a control, the bacterial cells were collected by washing and centrifuging in the same manner as above.

[0034] Prepare 100mL of 50mg / L lead ion solution in the conical flask, add the bacteria collected by the above method into the conical flask respectively, plug the bottle mouth with a cotton plug, treat at 30°C, 200rpm for 60min, calculate the adsorption rate, The adsorption rate of Saccharomyces cerevisiae without HCl and ethanol treatment to lead ions was 35.2%, and that of Saccharomyces cerevisiae treated with HCl and ethanol reached 78.6%, which was 1.23 times higher than that of the control.

Embodiment 3

[0036] Wash 0.5 g (dry weight) of Saccharomyces cerevisiae 3 to 4 times with 0.1 mol / L HCl, centrifuge at 3000 to 5000 rpm, and collect the bacteria. Using deionized water as a control, the bacterial cells were collected by washing and centrifuging in the same manner as above.

[0037] Prepare 100mL of 50mg / L copper ion solution in the conical flask, add the bacteria collected by the above method into the conical flask respectively, plug the bottle mouth with a cotton plug, treat at 30°C, 200rpm for 60min, calculate the adsorption rate, The Saccharomyces cerevisiae without HCl and ethanol treatment has an adsorption rate of copper ions of 40.9%, and the Saccharomyces cerevisiae treated with HCl and ethanol has an adsorption rate of copper ions of 81.4%, which is 0.99 times higher than that of the control.

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PUM

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Abstract

The invention discloses a method for improving adsorption capacity of saccharomyces cerevisiae for metal ions in food and belongs to the technical field of microorganisms. According to the method, saccharomyces cerevisiae is treated with HCl and ethanol, so that the adsorption effect of the saccharomyces cerevisiae on the heavy ions can be improved. The saccharomyces cerevisiae treated with the method has the adsorption capacity for lead ions, copper ions, rhodium ions and cobalt ions remarkably improved from 20% to 90% or above. The method is convenient to operate and low in cost and has important industrial application value.

Description

technical field [0001] The invention relates to a method for improving the ability of Saccharomyces cerevisiae to absorb metal ions in food, and belongs to the technical field of microorganisms. Background technique [0002] Heavy metals refer to metals with a specific gravity greater than 4 or 5. There are about 45 types, such as copper, lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver, etc. Although manganese, copper, zinc and other heavy metals are trace elements needed for life activities, most heavy metals such as mercury, lead, cadmium, etc. are not necessary for life activities, and all heavy metals exceeding a certain concentration will cause protein coagulation, which is harmful to the human body. Has a toxic effect. [0003] With the rapid development of the economy, a large amount of waste water is discharged, and the accumulation of heavy metals in the soil and water sources is intensified. The heavy metals flowin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
CPCA23L5/28
Inventor 贾雷
Owner ZIBO VOCATIONAL INST