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Preparation method of immobilized microorganisms embedded with aerogel

A technology for immobilizing microorganisms and aerogels, applied in the direction of being immobilized on/in organic carriers, etc., can solve the problems of easy loss of microorganisms, small growth space, weak anti-toxic ability, etc., and is conducive to growth and reproduction. , good biological affinity, low equipment requirements

Pending Publication Date: 2018-07-06
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is: in the existing microbial immobilization materials, a single immobilization method cannot well meet the mission of fixing functional microorganisms to repair environmental pollution, and aims at the easy loss of microorganisms in the immobilized materials, low biological activity, and resistance to environmental pollution. To solve the problems of weak toxicity, small growth space and short available time, a preparation method of immobilized microorganisms embedded in airgel is proposed

Method used

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  • Preparation method of immobilized microorganisms embedded with aerogel
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for immobilized microorganisms embedded in airgel, which comprises the steps of:

[0032] S1: Mix the monomers hydroxyethyl methacrylate, N-methylolacrylamide, polyacrylamide and diisodecyl adipate in a molar ratio of 1:3:2:1, and then mix the monomers with water The liquid is mixed evenly according to the volume ratio of 1:7;

[0033]S2: Add 0.5mL (1L of mixed solution) of tetramethylethylenediamine to the above-mentioned mixed solution as a catalyst, and then add 0.5g (1L of mixed solution) of persulfuric acid after magnetic stirring in an ice-water bath environment Ammonium was used as an initiator to react for 8h;

[0034] S3: Freeze the reacted gel in the refrigerator for 12 hours, gradually lower the temperature from -40°C to -80°C in four stages, each stage lowers the temperature by 10°C, and the time interval for changing the temperature between each stage is 3h , and finally lyophilized in a lyophilizer for 24 hours to make hydroxyethyl m...

Embodiment 2

[0041] A preparation method for immobilized microorganisms embedded in airgel, which comprises the steps of:

[0042] S1: Mix the monomers hydroxyethyl methacrylate, N-methylol acrylamide, polyacrylamide and diisodecyl adipate in a molar ratio of 2:3:3:1, and then mix the monomers with water The liquid is mixed evenly according to the volume ratio of 2:5;

[0043] S2: Add 0.6mL (1L of mixed solution) of tetramethylethylenediamine to the above-mentioned mixed solution as a catalyst, and then add 0.6g (1L of mixed solution) of persulfuric acid after magnetic stirring in an ice-water bath environment Ammonium was used as an initiator to react for 8h;

[0044] S3: Freeze the reacted gel in the refrigerator for 24 hours, gradually lower the temperature from -40°C to -80°C in four stages, each stage lowers the temperature by 10°C, and the time interval for changing the temperature between each stage is 6h , and finally lyophilized in a lyophilizer for 72 hours to make hydroxyethyl...

Embodiment 3

[0051] A preparation method for immobilized microorganisms embedded in airgel, which comprises the steps of:

[0052] S1: Mix the monomers hydroxyethyl methacrylate, N-methylol acrylamide, polyacrylamide and diisodecyl adipate in a molar ratio of 3:17:4:2, and then mix the monomers with water The liquid is mixed evenly according to the volume ratio of 2:9;

[0053] S2: Add 0.8mL (1L of mixed solution) of tetramethylethylenediamine to the above-mentioned mixed solution as a catalyst, and then add 1g (1L of mixed solution) of ammonium persulfate after magnetic stirring in an ice-water bath environment As an initiator to react for 9h;

[0054] S3: Freeze the reacted gel in the refrigerator for 24 hours, gradually lower the temperature from -40°C to -80°C in four stages, each stage lowers the temperature by 10°C, and the time interval for changing the temperature between each stage is 6h , and finally lyophilized in a lyophilizer for 36 hours to make hydroxyethyl methacrylate-hy...

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Abstract

The invention discloses a preparation method of immobilized microorganisms embedded with aerogel, and belongs to the fields of immobilized microorganism technology and environmental functional composite material preparation. The preparation method comprises the following steps: proportionally mixing monomer hydroxyethyl methacrylate, N-methylol acrylamide, polyacrylamide and diisodecyl adipate, and performing a series of treatment to prepare aerogel; and performing mixed culture on the prepared aerogel and bacterial liquid proportionally, uniformly mixing the bacterium-loaded aerogel with themixed liquid, then transferring the aerogel to a saturated calcium chloride solution to be crosslinked and hardened, and then cleaning the surfaces of immobilized spheres with sterile water to prepareimmobilized spheres embedded with the aerogel. The prepared immobilized microorganism spheres embedded with the aerogel have the advantages of large specific surface area, good mass transfer performance, good stability, high mechanical strength, low cost and the like and have wide application prospects in the aspect of environmental pollution remediation.

Description

technical field [0001] The invention relates to the technical field of immobilized microorganism technology and the preparation of environmental functional composite materials, in particular to a method for preparing immobilized microorganisms embedded in airgel. Background technique [0002] Immobilized microorganism technology is to treat one or two or more free microorganisms through physical and chemical means, so that they can be fixed in a specific space and maintain biological activity, which can be used repeatedly. The embedding method is currently the most common and widely studied method for preparing immobilized microorganisms. It is a method of diffusing microbial cells into a porous carrier, or encapsulating microorganisms when a water-insoluble polymer forms a gel. The embedding method is easy to operate and can maintain the multi-enzyme system in the organism. Common embedding materials include polyvinyl alcohol (PVA), polypropylene alcohol (ACAM), polyethyle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/10C12N11/04
CPCC12N11/04C12N11/08C12N11/10
Inventor 任洪强于清淼耿金菊
Owner NANJING UNIV
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