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Bacillus licheniformis serving as pesticide surface active agent and application thereof

A technology of Bacillus licheniformis and surfactant, which is applied in the fields of biological control and pesticide research and application, can solve problems such as unseen strains, and achieve the effects of being beneficial to storage, transportation and use, reducing use and reducing pollution

Active Publication Date: 2018-07-13
YANGZHOU UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

There are many bacterial strains of Bacillus licheniformis that can prevent and treat plant diseases, and there are also reports of Bacillus licheniformis with biosurfactant functions. A report on the cultivation of Bacillus licheniformis by fermentation of starch and other substrates for the development of pesticide preparations

Method used

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  • Bacillus licheniformis serving as pesticide surface active agent and application thereof
  • Bacillus licheniformis serving as pesticide surface active agent and application thereof
  • Bacillus licheniformis serving as pesticide surface active agent and application thereof

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Embodiment Construction

[0022] The present invention will be described in further detail below through specific examples.

[0023] (1) Isolation, screening and identification of Bacillus licheniformis W10

[0024] Tomato plants (roots with soil) were collected from vegetable fields in the suburbs of Yangzhou, and the top soil on the roots was shaken off after being brought back to the laboratory. The tomato roots still stuck to the fine soil were cut off, and the collected rhizosphere soil samples were collected.

[0025] Soil microorganisms were isolated using the plate dilution coating method. Cut the collected tomato root system into small sections of about 4mm, weigh 10g and put it into a conical flask filled with 90mL sterile water, vibrate at 160r / min for 30min, and obtain 10 -1 Concentration of soil dilution solution, and then sequentially diluted 10 -3 -10 -7 Concentration gradient soil suspension. Draw 100 μL and spread on NA medium (peptone 10g, beef extract 3g, sodium chloride 5g, agar...

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Abstract

The invention relates to bacillus licheniformis serving as a pesticide surface active agent and application thereof, and belongs to the field of biological prevention and control and pesticide research and application in the plant protection subject. A bacterial strain is a high-efficiency biocontrol bacterium-bacillus licheniformis W10 bacterial strain which is separated and screened from plant rhizosphere soil, the preservation number of the bacterial strain in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 14859. According to bacillus licheniformis, base materials, such as soybean cake powder, corn starch and the like, serve as a fermentation medium, the prepared bacterial strain fermentation broth microbial inoculum can improve the surface tension of pesticide, reduce the contact angle, has a good surface active agent function, and meanwhile, has the effect of preventing and controlling plant diseases. Therefore, the fermentation broth microbial inoculum can not only serve as the surface active agent to be applied to processing and preparation of pesticide suspending agents, but also can prevent and control diseases, and presents a broad application prospect.

Description

technical field [0001] The invention relates to the surface activity of bio-control bacterial fermentation culture, and belongs to the field of bio-control and pesticide research and application in the field of plant protection. It specifically relates to a bacillus licheniformis used as a pesticide surfactant and an application thereof. Background technique [0002] Bacillus spp., as an important class of biocontrol bacteria, can produce a variety of antibacterial and antibacterial substances, including bacteriocins, cell wall degrading enzymes, antibacterial proteins, lipopeptide antibiotics, polyketides and polypeptides etc., have the potential to prevent and control plant diseases, and are also the main source of biological control bacteria. At present, researchers focus on various antibacterial active substances in the screening of biocontrol bacteria. However, there are many kinds of fermentation metabolites of biocontrol bacteria. In addition to producing antibacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N25/30A01N63/02A01P3/00C12R1/07
CPCA01N25/30A01N63/10C12N1/205C12R2001/07
Inventor 纪兆林朱薇李春骁童蕴慧金唯新王友德
Owner YANGZHOU UNIV
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