Preparation method of fibroin aquagel film

Abstract

The invention provides a preparation method of a fibroin aquagel film. Natural silk is added into a sodium carbonate solution; stirring reaction is performed for 30 to 50 min at the temperature of 100to 110 DEG C; the operation is repeated for 3 to 5 times to obtain pure fibroin fiber; lithium bromide, butyl oxide and water are mixed and are subjected to ultrasonic treatment for 5 to 10 min to obtain a uniform mixed solution A; the pure fibroin fiber is added into the mixed solution A; dissolution reaction is performed for 30 to 50 min at the temperature of 80 to 120 DEG C; then, dialysis andfiltering are performed to obtain a fibroin water solution; a polyvinyl alcohol solution is prepared; nanometer titanium dioxide, elastin, the fibroin water solution and the polyvinyl alcohol solution are mixed; the reaction treatment is performed for 3 to 8h at the temperature being 60 to 80 DEG C and the pH being 7.3 to 8.1; obtained products are subjected to spreading flowing to realize the flat spreading in a shaping mode; freeze drying is performed at the temperature of -30 to -10 DEG C to obtain the fibroin aquagel film.

Description

technical field [0001] The invention relates to the technical field of production of silk fibroin membranes, in particular to a preparation method of silk fibroin hydrogel membranes. Background technique [0002] Silk is one of the earliest natural proteins used by humans. As a natural filament fiber with excellent performance, it is mainly used in the field of textiles. Silk is a natural long fiber protein composed of silk fibroin and sericin. Sericin is coated on the outside of silk fibroin, accounting for about 25% of the weight; silk is a tough and elastic protein, and there are crystals in its macromolecules. region and non-crystalline region, the main components are tyrosine, alanine, serine, proline, tyrosine and other amino acids; the amino acid composition of silk fibroin is mainly glycine, alanine and serine, Accounting for about 85%, it is similar to the amino acid composition of human collagen, has good biocompatibility, and can be prepared into materials in var...

AI Technical Summary

Problems solved by technology

[0003] After degumming and dissolving, silk fibroin becomes a water-soluble material. After pure silk fibroin is prepared into a film or other forms, it is brittle and its mechanical properties are relatively poor. In order to take advantage of its good biocompatibility, people have been In the study of improving its mechanical properties, the main way to improve its mechanical properties is to add other polymer materials to silk fibroin to induce changes in the structure of silk fibroin and improve its mechanical properties, including the improvement of its water solubility, but there are still some problems. Big deficiency