Circular dumbbell-shaped probes and application thereof

A dumbbell-shaped, probe-based technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of reduced cell activity, slow reaction rate, and limited accuracy, so as to reduce false positives and background signals, The effect of improving detection accuracy and precision
CN108300775AActive Publication Date: 2018-07-20SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2018-07-20

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Abstract

The invention discloses circular dumbbell-shaped probes and an application thereof. Two kinds of circular dumbbell-shaped probes are designed for chain hybridization reaction amplification, so as to detect the concentration of a to-be-tested target and to be applied to in-situ imaging of target sequences in cells. Compared with the existing chain hybridization amplification method, the method hasthe advantages that the defect that linear probes or hairpin probes are easy to hydrolyze in cells is overcome, high hydrolysis resistance of circular nucleic acid probes is discovered, the circular probes are combined with a chain hybridization reaction to be applied to imaging of target sequences in living cells, false positive and background signals are reduced, and detection accuracy is improved.
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Description

technical field

[0001] The invention belongs to the technical field of molecular detection, and in particular relates to a circular dumbbell-shaped probe and an application thereof. Background technique

[0002] MiRNA is a class of non-coding small molecule RNA (18-24 bases) that regulates gene expression. It plays an important role in the growth, development, differentiation and reproduction of animals and plants. About 30% of human genes are regulated by miRNA, and the expression level of miRNA is closely related to major human diseases. Quantitative detection of miRNA is helpful for in-depth understanding of its mechanism of action, which is of great significance to the diagnosis and treatment of diseases and the development of related gene medicines. Due to the short sequence of miRNA, the expression level in cells or body fluids is very low, easy to degrade, and the difference between homologous miRNAs is only 1-2 bases, it is difficult to detect by general methods; ...

Claims

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