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Application of composition containing MG 53 mutant in preparation of neuroprotective drugs

A neuroprotection and nerve cell protection technology, applied in the field of biopharmaceuticals, can solve the problems of inability to protect and repair endogenous MG53 protein cells, and no MG53 protein expression is found, and achieve good promotion and application prospects, improve learning, and stimulate angiogenesis. Effect

Pending Publication Date: 2018-07-24
MUDANJIANG YOUBO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They work together to bind to proteins that are no longer needed by cells, and mark these proteins with ubiquitin for degradation. Therefore, it has a good application prospect, but it is reported that it has obvious curative effect only in the failure and repair of multiple organs such as heart muscle, lung, kidney, bone, skin, etc., and the expression of MG53 protein has not been found in the brain, that is to say, it cannot By activating endogenous MG53 protein activity to exert its cellular protection and repair function, it can only rely on exogenous introduction to play a role, so for the development of a composition containing MG53 or its mutants, it can be used to enter the blood brain It plays a significant role in various neurological diseases that seriously threaten human health, and will be of great benefit to the development of human health

Method used

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  • Application of composition containing MG 53 mutant in preparation of neuroprotective drugs
  • Application of composition containing MG 53 mutant in preparation of neuroprotective drugs
  • Application of composition containing MG 53 mutant in preparation of neuroprotective drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Nerve cell protection experiment

[0039] (1) Experimental principle:

[0040] h 2 o 2 The oxidative damage of cells has become one of the important methods to study the oxidative damage of nerve cells.

[0041] The degree of differentiation of SH-SY5Y cells is low, and the cell morphology, physiology and biochemical functions are similar to normal nerve cells, and have obvious axons. 2 o 2 Highly sensitive to induction of oxidative stress, H 2 o 2 Induced SH-SY5Y cell oxidative damage model is widely used in the study of oxidative stress-induced neuronal cell degeneration and apoptosis.

[0042] (2) Experimental materials

[0043] SH-SY5Y cells: purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences;

[0044] 1640 medium and FBS (Fetal Bovine Serum): purchased from Gibco;

[0045] MG53 mutant protein: obtained from strains constructed, fermented and purified by our company, batch number 201...

Embodiment 2

[0055] Example 2: Effects of compositions containing MG53 mutants on cerebral ischemia-reperfusion in rats

[0056] (1) Experimental principle

[0057] Cerebral arteries come from bilateral internal carotid arteries and vertebral arteries, form a basilar artery ring at the base of the brain, divide into anterior, middle, and posterior cerebral arteries, branch in the pia mater and enter the human brain parenchyma, so cerebral blood vessels are further divided into cerebral parenchymal vessels and Extraparenchymal blood vessels. Blocking the blood vessels that control the brain tissue can simulate a pathological model similar to human stroke, which can be used for pathological, physiological, biochemical research and drug evaluation. Clinical and experimental cerebral ischemia includes global cerebral ischemia and partial cerebral ischemia, which can be further divided into temporary ischemia and permanent ischemia. The middle cerebral artery is the most common occlusion of h...

Embodiment 3

[0092] Example 3: Effects of MG53 Mutant Composition on Rapid Aging Brain Atrophy and Dementia Model Mouse Model

[0093] (1) Experimental principle

[0094] Alzheimer's disease (AD) is a progressive dementia-based brain degenerative disease that accompanies aging. Its main clinical manifestations are memory loss, mental decline, and behavioral personality degeneration. So far, there is still a lack of effective prevention and treatment measures. Behavioral experiment is a common experimental method for studying animal behavior changes. It is usually used to observe and study abnormal changes or performances in the nervous, mental and psychological aspects. It is of great significance in basic research and is also one of the basic methods for judging dementia. one. The shuttle box experiment is an important means to quantitatively test the changes in animal behavior. The animal model used in this example is the natural rapid aging model mouse SAM (SenecenceA °elerateMouse) i...

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Abstract

The invention discloses application of a composition containing an MG 53 mutant in preparation of neuroprotective drugs. The research results prove that the MG53 mutant has a prominent neuroprotectiveeffect and can cross the blood-brain barrier, thus being applied to prevention or treatment of multiple neurodegenerative diseases such as ischemic stroke, brain atrophy, senile dementia, multiple system atrophy, brain atrophy, motor neuron diseases, ataxia telangiectasia, spinal muscular atrophy and optic atrophy.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to the application of a composition containing MG53 mutants in the preparation of neuroprotective drugs. Background technique [0002] In recent years, various neurological diseases have seriously endangered human life and health, including ischemic stroke and various neurodegenerative diseases, such as brain atrophy, Alzheimer's disease, multiple system atrophy, motor neuron disease, ataxia capillary Dilatation, spinal muscular atrophy, and optic atrophy have become common and frequently-occurring diseases that cause disability and death, and bring heavy burdens to society and families. [0003] Ischemic stroke, accounting for about 85% of all strokes, is a disease caused by changes in the normal function of cells due to reduced blood flow in the brain. In humans, if the brain is ischemic, the brain tissue responds quickly to the ischemia. Short-term ischemia of neur...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P9/10A61P9/14A61P25/00A61P25/28A61P21/00
CPCA61K38/1709
Inventor 慎东
Owner MUDANJIANG YOUBO PHARMA CO LTD
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