The invention discloses a 1, 3, 4-oxadiazole derivative, and discloses different preparation methods of the 1, 3, 4-oxadiazole derivative and an application thereof. The compound can be used for preparing inhibitors and anti-tumor drugs of ataxia telangiectasia mutant genes and Rad3 related kinase (ATR). The 1, 3, 4-oxadiazole derivative disclosed by the invention is novel in structure, and an electron withdrawing group (nitryl) of a side-chain benzene ring is beneficial to improving the anti-tumor activity of the 1, 3, 4-oxadiazole derivative. Compared with a traditional preparation method of the 1, 3, 4-oxadiazole derivative, the preparation method of the 1, 3, 4-oxadiazole derivative can overcome the defects that under the high-temperature reaction condition, a dehydration cyclizing agent is high in toxicity, prone to corrosion and the like, dibromotriphenylphosphine is adopted as a dehydration cyclizing agent in the preparation method, reaction can be conducted at the room temperature, and the preparation method is environmentally friendly.