Floating bead flotating and collecting method of microalgae

A harvesting method, microalgae technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high equipment requirements, high cost, low recovery rate, etc., to achieve increased recovery rate, Effects of reduced loss and uniform particle size

Inactive Publication Date: 2018-07-24
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen from Table 1 that traditional microalgae harvesting methods such as pressure filtration and centrifugation have low recovery rates and high energy consumption, while vacuum filtration, electrocoagulation and dissolved air flotation Although the recovery efficiency is very high, the energy consumption is high, the equipment requirements are high and the cost is relatively expensive

Method used

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  • Floating bead flotating and collecting method of microalgae
  • Floating bead flotating and collecting method of microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0026] This embodiment includes the following steps:

[0027] Step 1. Place Chlorella in a photobioreactor loaded with BG-11 medium, at a temperature of (25±1)°C, a light intensity of 3000Lux to 3500Lux, a light-dark cycle of 12L:12D, and an air flow of 15L / h, cultivate under the conditions of pH 7-7.5, and then select the chlorella suspension in the static growth period as the harvesting raw material;

[0028] 40mg chitosan flocculant was added to 2L chlorella suspension, then stirred for 1min under the condition of 250rpm, and then left to stand at room temperature for 15min to obtain the pretreated chlorella suspension; the chlorella The concentration of the suspension is 7.6×10 6 cells / mL;

[0029] Step 2. Disperse 1000 mg of floating beads in 500 mL of deionized water to form a floating bead dispersion system; the floating beads have a hollow structure, the material of the floating beads is sodium borosilicate, and the density of the floating beads is 0.35 g / cm 3 , the...

Embodiment 2

[0033] This embodiment includes the following steps:

[0034] Step 1. Place Chlorella in a photobioreactor loaded with BG-11 medium, at a temperature of (25±1)°C, a light intensity of 3000Lux to 3500Lux, a light-dark cycle of 12L:12D, and an air flow of 15L / h, cultivate under the conditions of pH 7-7.5, and then select the chlorella suspension in the static growth period as the harvesting raw material;

[0035] Add 100mg of aluminum sulfate flocculant to 2L of chlorella suspension, then stir for 1min under the condition of 250rpm, and then let stand at room temperature for 15min to obtain the pretreated chlorella suspension; the chlorella suspension The concentration of liquid is 7.6×10 6 cells / mL;

[0036] Step 2. Disperse 1000 mg of floating beads in 500 mL of deionized water to form a floating bead dispersion system; the floating beads have a hollow structure, the material of the floating beads is sodium borosilicate, and the density of the floating beads is 0.35 g / cm 3 ...

Embodiment 3

[0040] This embodiment includes the following steps:

[0041] Step 1. Place Chlorella in a photobioreactor loaded with BG-11 medium, at a temperature of (25±1)°C, a light intensity of 3000Lux to 3500Lux, a light-dark cycle of 12L:12D, and an air flow of 15L / h, cultivate under the conditions of pH 7-7.5, and then select the chlorella suspension in the static growth period as the harvesting raw material;

[0042] 200mg of ferric sulfate flocculant was added to 2L of Chlorella suspension, then stirred for 1min under the condition of 250rpm, and then left to stand at room temperature for 15min to obtain pretreated Scenedesmus dimorphus suspension; said Scenedesmus dimorphus The concentration of the algae suspension was 15.8×10 6 cells / mL;

[0043] Step 2: Disperse 1200 mg of floating beads in 500 mL of deionized water to form a floating bead dispersion system; the floating beads have a hollow structure, the material of the floating beads is sodium borosilicate, and the density o...

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Abstract

The invention discloses a floating bead flotating and collecting method of microalgae. The floating bead flotating and collecting method comprises the following steps: 1, adding a flocculant into a microalga suspension, and then stirring and standing to obtain the pretreated microalga suspension; 2, dispersing floating beads in deionized water to form a floating bead dispersion system; 3, adding the floating bead dispersion system into the pretreated microalga suspension to obtain a mixed suspension, stirring and standing the mixed suspension, and then overflowing to obtain floating bead-microalga combination containing an overflowing liquid; 4, centrifuging the floating bead-microalga combination containing the overflowing liquid, and then collecting precipitates to obtain the microalgae.By the floating bead flotating and collecting method, the microalgae are adsorbed by the floating beads with stable morphology, so that the contact probability and the contact area of the floating beads and the microalgae are increased, the adhesion efficiency of the microalgae is greatly improved, therefore, the collecting rate of the microalgae is improved; in addition, bubbles are not requiredto be generated in the collecting process, the equipment investment is reduced, the collecting energy consumption is reduced, and the collecting cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of separation of microalgae, and in particular relates to a method for harvesting microalgae by floating bead flotation. Background technique [0002] Microalgae are a class of tiny organisms that grow in water with a wide variety and a wide distribution. Microalgae are the earth's inorganic carbon (CO 2 ) and inorganic nitrogen (NO x ) into organic carbon (sugars and oils) and organic nitrogen (protein) is the most efficient organism. The growth process of microalgae can realize wastewater purification and CO 2 The emission reduction is highly coupled, and has low requirements for the growth environment, and does not compete with crops for land and water. Therefore, microalgae are recognized as the future alternative to fossil resources to obtain bioenergy, bio-based materials, bio-based chemicals and health pharmaceutical products. The most promising raw material. The use of microalgae to prepare high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 李彦鹏邹小彤徐开伟文豪王金龙薛雅婷赵双峰乔龙凯
Owner CHANGAN UNIV
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