PCR (Polymerase Chain Reaction) specific primer and kit for detecting thyroid cancer metastasis associated gene, and using method of kit

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) specific primer and a kit for detecting a thyroid cancer metastasis associated gene, and a using method of the kit. The thyroid cancer metastasis associated gene related to PCR specific primer and the kit, which are independently researched and developed and are based on a high throughput sequencing technology, for detecting the thyroid cancer metastasis associated gene and the using method of the kit is subjected to sample validation, the PCR specific primer, the kit and the using method can be used for detecting a mutation situation of the thyroid cancer metastasis associated gene, a detection result can be used as an intermediate result together with other detecting results for judging whether cancer cells are metastasized or notor the cancer cells have the possibility of metastasis or recurrence, and an important significance in treatment and prognosis of thyroid cancer is obtained; in addition, limit of a traditional detecting technology is broken through by utilizing the high throughput sequencing technology, the detection sensitivity is increased, and even low-abundance mutant genes can also be detected, so that theimportant significance in aided prediction on spreading and recurrence of thyroid cancer cells is obtained.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a PCR-specific primer for detecting thyroid cancer metastasis-related genes, a kit and a use method thereof. Background technique [0002] The thyroid is the largest endocrine organ in the human body, and thyroid cancer is a common endocrine tumor. Thyroid tumors are divided into benign and malignant, among them, malignant thyroid tumors include papillary carcinoma, follicular carcinoma, medullary carcinoma and undifferentiated carcinoma. Differentiated thyroid cancer accounts for 90% of all thyroid cancers. Undifferentiated thyroid cancer is rare, but it has a high degree of malignancy and poor prognosis. [0003] In the past 30 years, the incidence of thyroid cancer in most regions of the world, including my country, has been on the rise. In 2010, the incidence rate of thyroid cancer in my country was 4.12 / 100,000, male was 1.93 / 100,000, and female was 6.42 / 100,000; the morta...

AI Technical Summary

Problems solved by technology

Conventional serial section HE staining method has low sensitivity and needs to cut dozens or even hundreds of sections, which has been rarely used in recent years; immunohistochemical method is relatively simple to operate, with high sensitivity and specificity, but it is time-consuming and prone to False positive and false negative; the advantages of flow cytometry are fast speed, high precision, and good accuracy, but the disadvantage is that specific fluorescent dyes are required and the cost of instruments and reagents is high; PCR / RT-PCR technology is amplified Tumor cell marker genes or target RNA are used to detect tumor metastasis. The biggest advantage is high sensitivity, but the PCR method can only be designed for one or several known mutation sites, and cannot be used to detect unknown mutations; Qualcomm Quantitative sequencing is a next-generation sequencing technology that can perform high-throughput sequencing of multiple samples. It is time-consuming, high-precision, and large in data volume. It can detect both known mutation sites and unknown mutations.

[0006] Although high-throughput sequencing technology has been widely used in the detection of unknown mutations, there are still no effective, accurate and easy-to-use targeted mutations due to the limitations of numerous genes related to thyroid cancer metastasis and the design of amplification primers. PCR-specific primers for detection of genes related to metastasis of thyroid cancer and related detection methods

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