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Use of mtDNA detection reagent in preparation of diagnostic kits for renal injury degree or transplant kidney function and diagnostic kits

A technology of diagnostic kits and detection reagents, applied in the medical and health field, can solve the problems of increasing the risk of kidney transplantation, increasing the waiting time for surgery, prolonging the waiting time, etc., so as to achieve a simple and easy diagnosis process, high reliability of the diagnosis effect, and high detection efficiency. cost reduction effect

Inactive Publication Date: 2018-07-31
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing detection methods have the following disadvantages: (1) invasiveness: cutting out part of the donor kidney tissue will damage the kidney and increase the risk of kidney transplantation; (2) high cost: perform a biopsy of the transplanted kidney , need to perform pathological experiments; (3) Prolonged waiting time: During the pathological examination process of donor needle biopsy, the waiting time for surgery is increased; (4) High technical requirements: Professional pathologists are required to read the films

Method used

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  • Use of mtDNA detection reagent in preparation of diagnostic kits for renal injury degree or transplant kidney function and diagnostic kits
  • Use of mtDNA detection reagent in preparation of diagnostic kits for renal injury degree or transplant kidney function and diagnostic kits
  • Use of mtDNA detection reagent in preparation of diagnostic kits for renal injury degree or transplant kidney function and diagnostic kits

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a diagnostic kit for the degree of kidney injury, which includes detection primers, QIAamp kit and qPCR reagent.

[0044]Wherein, the detection primers include a first primer pair and a second primer pair, the base sequence of the first primer pair is shown in SEQ NO.1-2, and the base sequence of the second primer pair is shown in SEQ NO.3-4; qPCR reagents include PCR reaction buffer and Taq DNA polymerase.

Embodiment 2

[0046] This embodiment provides a diagnostic kit for the degree of kidney injury, which includes detection primers, QIAamp kit and qPCR reagents.

[0047] Wherein, the detection primers include a first primer pair and a second primer pair, the base sequence of the first primer pair is shown in SEQ NO.1-2, and the base sequence of the second primer pair is shown in SEQ NO.3-4; qPCR reagents include PCR reaction buffer, Taq DNA polymerase and dNTPs.

Embodiment 3

[0049] This embodiment provides a diagnostic kit for the degree of kidney injury, which includes detection primers, QIAamp kit and qPCR reagents.

[0050] Wherein, the detection primers include a first primer pair and a second primer pair, the base sequence of the first primer pair is shown in SEQ NO.1-2, and the base sequence of the second primer pair is shown in SEQ NO.3-4; qPCR reagents include PCR reaction buffer, Taq DNA polymerase, dNTPs and Mg 2+ .

[0051] It should be noted that the QIAamp kit mentioned in the embodiment of the present invention is an existing kit for extracting nucleotides, and its specific use process and components will not be repeated here.

[0052] The diagnostic kit for the degree of donor kidney injury in Example 1 was used in the diagnosis process of the degree of donor kidney injury and the function of the transplanted kidney.

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Abstract

The invention discloses use of an mtDNA detection reagent in preparation of diagnostic kits for renal injury degree or transplant kidney function and the diagnostic kits and relates to the technical field of medical treatment and health. Reagents for detecting mtDNA in blood plasma are applied to the preparation of the diagnostic kits for the renal injury degree and the diagnostic kits for the transplant kidney function, diagnostic processes for the renal injury degree and the transplant kidney function can be simpler, more convenient and easier, nephridial tissue is not required to be cut fordetection, the detection is a noninvasive drug detection method, and the detection cost is remarkably reduced. The diagnostic kits for the renal injury degree and the diagnostic kits for the transplant kidney function, provided by the invention, separately comprise the reagents for detecting the mtDNA in the blood plasma, the diagnosis effects are high in reliability, and the diagnosis cost is low.

Description

technical field [0001] The invention relates to the field of medical and health technology, and particularly relates to the use of the mtDNA detection reagent in preparing a diagnostic kit for the degree of kidney damage or transplanted kidney function and the diagnostic kit. Background technique [0002] Kidney transplantation is the most effective way to treat patients with end-stage renal disease. The quality of the donated kidney not only plays an important role in the distribution of kidneys, but also predicts the recovery speed of the transplanted kidney function and the long-term survival of the recipient. Creatinine levels play an important role. At present, in the evaluation of the quality of the donor kidney, the zero-point puncture of the donor kidney is a common method for judging the quality of the donor kidney. The zero-point puncture means that after the donor kidney is obtained, part of the kidney tissue is cut out for frozen histopathological examination. A...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883
Inventor 孙启全韩飞
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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