Unlock instant, AI-driven research and patent intelligence for your innovation.

DNA fragment detection system with high sensitivity, rapidity and absolute quantification as well as detection method thereof

A detection system and absolute quantification technology, applied in the field of DNA detection, can solve the problems of inability to make absolute quantification, long detection time, and low sensitivity, and achieve the effects of reducing target DNA amplification multiples, short process time, and high sensitivity

Inactive Publication Date: 2018-08-03
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF20 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is that the isothermal nucleic acid detection device in the prior art takes a long time to detect, has low sensitivity and cannot be absolutely quantified, and then provides a highly sensitive, fast and absolutely quantitative DNA fragment detection system and detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA fragment detection system with high sensitivity, rapidity and absolute quantification as well as detection method thereof
  • DNA fragment detection system with high sensitivity, rapidity and absolute quantification as well as detection method thereof
  • DNA fragment detection system with high sensitivity, rapidity and absolute quantification as well as detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This embodiment provides a high-sensitivity, rapid, and absolute quantitative DNA fragment detection system, including a DNA amplification module for carrying a DNA amplification system, including at least 20,000 independent reaction units, and each reaction unit can hold liquid The volume is less than 1nL; the temperature control module is used to control the reaction temperature of the DNA amplification module; the detection module is used for laser scanning to detect the amplification result in the DNA amplification module in real time.

[0065] The DNA amplification module, such as figure 1 and figure 2 As shown, the DNA amplification module includes a chip base 1, a top cover 2 and a microwell array chip 3; a groove is provided in the middle of the chip base 1 for placing the microwell array chip 3, and the top cover 2 covers the microwell array chip 3. On the chip 3 , the upper surface of the closed upper cover 2 is on the same plane as the upper surface of the ...

Embodiment 2

[0079] This embodiment provides a digital PCR chip, including a temperature control sample pool 9, such as Figure 3-Figure 5 As shown, the temperature control sample cell 9 includes a base 5 and a transparent window 8. There is a through hole in the middle of the base 5, and the transparent window 8 is embedded in the through hole. The material of the transparent window 8 is glass or polymethyl methacrylate. One of them can make the light pass from the bottom of the base 8 to the top of the transparent window 8 through the transparent window 8. Heating ceramics 6 are installed on the left and right sides of the transparent window 8, the upper surface of the heating ceramic sheet 6 is flush with the upper surface of the transparent window 8, the base 5 is provided with a sample groove, and the heating ceramics 6 and the transparent window 8 are arranged on The bottom surface of the sample tank, the upper surface of the heating ceramic 6 and the upper surface of the transparent...

Embodiment 3

[0082] This embodiment provides a high-sensitivity, rapid, and absolute quantitative DNA fragment detection system, including a DNA amplification module for carrying a DNA amplification system, including at least 20,000 independent reaction units, and each reaction unit can hold liquid The volume is less than 1nL; the temperature control module is used to control the reaction temperature of the DNA amplification module; the detection module is used for laser scanning to detect the amplification result in the DNA amplification module in real time.

[0083] The DNA amplification module, such as figure 1 and figure 2As shown, the DNA amplification module includes a chip base 1, a top cover 2 and a microwell array chip 3; a groove is provided in the middle of the chip base 1 for placing the microwell array chip 3, and the top cover 2 covers the microwell array chip 3. On the chip 3 , the upper surface of the closed upper cover 2 is on the same plane as the upper surface of the c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA fragment detection system with high sensitivity, rapidity and absolute quantification. The DNA fragment detection system comprises a DNA amplification module used for bearing a DNA amplification system, wherein the DNA amplification module comprises at least twenty thousands of independent reaction units, each reaction unit is filled with liquid having a volume beingless than 1 nL; a temperature control module used for controlling the reaction temperature of the DNA amplification module; and a detection module used for performing real-time detection on the amplification results in the DNA amplification module through laser scanning. The invention also discloses a DNA fragment detection method with high sensitivity, rapidity and absolute quantification, The disclosed detection system and the detection method have the advantages of short time consuming and high sensitivity, and can carry out absolute quantitative determination.

Description

technical field [0001] The invention belongs to the field of DNA detection, and in particular relates to a high-sensitivity, rapid and absolute quantitative DNA fragment detection system and detection method. Background technique [0002] DNA detection technology is the detection and analysis of DNA molecular structure, content and sequence, and is the basis for research and application in the field of biomedicine. A major challenge of DNA testing is to obtain DNA information of very rare samples, such as DNA in ancient biological fossils, human hair, rare DNA fragments in blood, etc., which must rely on DNA amplification technology. Polymerase chain reaction (PCR) technology is a molecular biology technique used to amplify specific DNA fragments. In 1983, Kary Mullis of PE Cetus Company in the United States established PCR technology, which was then widely used in molecular biology, genetic engineering and other fields related to DNA identification. With the continuous de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/38C12M1/34C40B40/06C12Q1/6844C12Q1/686
CPCC12Q1/6844C12Q1/686C40B40/06
Inventor 周连群李金泽张芷齐李传宇姚佳张威郭振
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI