Lactobacillus plantarum CCFM1019, fermented food and application to preparation of drug

A technology of CCFM1019 and Lactobacillus plantarum, applied in the field of microorganisms, can solve problems such as unsatisfactory adsorption effect, achieve good gastric acid resistance and bile salt resistance, reduce damage, and promote discharge

Active Publication Date: 2018-08-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing literature reports, the highest adsorption rate of probiotics to DEHP in vitro is only 9.62%, and the adsorption effect is not ideal.

Method used

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  • Lactobacillus plantarum CCFM1019, fermented food and application to preparation of drug
  • Lactobacillus plantarum CCFM1019, fermented food and application to preparation of drug
  • Lactobacillus plantarum CCFM1019, fermented food and application to preparation of drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Lactobacillus plantarum CCFM1019 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Lactobacillus plantarum CCFM1019 was streak-inoculated in the MRS solid medium, and cultured aerobically at 37°C for 24 hours, and then subcultured in the MRS medium for 2 to 3 times, then the culture medium of Lactobacillus plantarum CCFM1019 was taken, The bacteria were collected by centrifugation at 8000×g for 5 min, resuspended in (1:1) artificial simulated gastric juice of pH 2.5 (MRS medium containing 1% pepsin, pH=2.5), and then cultured aerobically at 37°C. Samples were taken at the beginning (0h), 1h, 2h and 3h, and MRS agar medium was used to pour culture for plate colony counting, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the number of viable bacteria in the culture medium to the number of viable bacteria at 0h, expressed in %.

[0070] Take the culture sol...

Embodiment 2

[0075] Example 2: Lactobacillus plantarum CCFM1019 has good adsorption capacity for DEHP and MEHP in an aqueous solution containing plasticizer DEHP or MEHP in vitro

[0076] Bacteria adsorption: After the experimental bacteria were purified and activated, they were inserted into the MRS liquid medium according to the inoculation amount of 1% (v / v), and cultured at 37°C for 20h (E.coli uses LB culture based on shaking culture at 37°C; E. .faecalis is cultured in MRS based on 37°C), then centrifuged at 8000×g for 20 minutes, poured off the supernatant, resuspended with ultrapure water, continued to centrifuge at 8000×g for 20 minutes, and poured off the supernatant Live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 50mg / LDEHP or 10mg / L MEHP aqueous solution, and the final bacterial concentration reached 1g wet bacteria / L, and the blank control was wet bacteria resuspended in ultrapure water without DEHP and MEHP. The volume of each group is 1 mL. ...

Embodiment 3

[0083] Example 3: Lactobacillus plantarum CCFM1019 has no acute toxic side effects on SD rats

[0084] Lactobacillus plantarum CCFM1019 was resuspended in 2% (w / v) sucrose solution, and the cell density was 1.0×10 9 CFU / mL. Take 10 healthy male SD rats with a body weight of about 100 g, and give 2 mL of the suspension solution of this concentration by intragastric administration every day, observe for a week, and record the death and body weight.

[0085] The results of these tests are listed in Table 4. The results showed that the feeding concentration of 1.0×10 9 CFU / mL of Lactobacillus plantarum CCFM1019 had no significant impact on rats, no significant change in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

[0086] Table 4 Changes in body weight and death of rats

[0087]

[0088] Note: -: Rats did not die

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Abstract

The invention discloses lactobacillus plantarum CCFM1019, fermented food and application to preparation of a drug. The lactobacillus plantarum is apparently better than the residential bacteria of human intestines such as escherichia coli and enterococcus faecalis in the efficacy aspect for promoting the discharging of DEHP and MEHP and also better than the commercial bacterial strain lactobacillus rhamnosus LGG. The lactobacillus plantarum CCFM1019 of the invention can be used as an effective means for preventing and alleviating the body injury caused by the DEHP and MEHP, and does not have the toxic and side effect of the drug. The actobacillus plantarum CCFM1019 can be used for preparing a drug composition and fermented food for alleviating and preventing DEHP and metabolism product toxicity, and has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to plant lactobacillus CCFM1019, its fermented food and its application in the preparation of medicines. Background technique [0002] Bis(2-ethylhexyl) phthalate (hereinafter referred to as DEHP), as a plasticizer, can be added to plastics to increase their flexibility and plasticity. Due to its low price, DEHP is widely used in the production of plastic products such as medical equipment and chemical products, and is currently the most widely used plasticizer in my country. In these plastic products, DEHP is mainly combined with other molecules in the form of hydrogen bonds and van der Waals forces rather than covalent bonds, so it is easy to escape during use, thereby migrating to the environment and even the human body. Health hazard. [0003] Studies have shown that the absorption and distribution of DEHP in the human body lasts for about 4-8 hours, and most of them can be c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/02A23L29/00C12R1/25
CPCA61P39/02A23L29/065A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30Y02A50/30
Inventor 陈卫王刚陈潜赵建新张灏
Owner JIANGNAN UNIV
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