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Method for selecting high-cordycepin cordyceps militaris strain through ultraviolet-induced conidia

A technology of conidia and high cordycepin, which is applied in the direction of using spores, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low induction efficiency, etc., and achieves a simple and easy way, and the colony grows Cordyceps militaris. The effect of the fruiting body is strong and the workload is small

Inactive Publication Date: 2018-08-10
CHANGDE YANDI BIOTECH LTD CO +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention relates to a method for screening high cordycepin strains by inducing conidia by ultraviolet rays, which solves the problem of low induction efficiency of previous methods for inducing mycelium

Method used

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  • Method for selecting high-cordycepin cordyceps militaris strain through ultraviolet-induced conidia
  • Method for selecting high-cordycepin cordyceps militaris strain through ultraviolet-induced conidia
  • Method for selecting high-cordycepin cordyceps militaris strain through ultraviolet-induced conidia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Under sterile conditions, use a pipette gun to draw 200 microliters of mature Cordyceps militaris liquid strain (the strain is derived from the megaspore head variety of Changde Yandi Biotechnology Co., Ltd. strain bank), and inoculate it into PDA culture On the base, spread the mycelium evenly with a coating stick;

[0026] (2) Transfer the PDA plate coated with mycelium to a biochemical incubator, and incubate in the dark at 19-21°C for 4-5 days, so that the mycelium is covered with the entire PDA plate;

[0027] (3) Set the light intensity to 500-2000 lux, and see the light change color for 3-4 days, so that the white mycelium turns orange-yellow;

[0028] (4) In aseptic conditions, invert the PDA plate to an angle of 45°, pipette the sterile water to gently rinse the top of the mycelium 5 to 10 times, and collect the mixture;

[0029] (5) Dispense the collected mixture into 10ml EP tubes, set at 4°C, centrifugal force 3000-5000g, and centrifuge for 10-15min to ...

Embodiment 2

[0036] (1) Under sterile conditions, use a pipette gun to draw 200 microliters of mature Cordyceps militaris liquid strains (the strains are derived from the species of Acorns grass in the strain bank of Changde Yandi Biotechnology Co., Ltd.), and inoculate them into PDA culture On the base, spread the mycelium evenly with a coating stick;

[0037] (2) Transfer the PDA plate coated with mycelium to a biochemical incubator, and incubate in the dark at 19-21°C for 4-5 days, so that the mycelium is covered with the entire PDA plate;

[0038] (3) Set the light intensity to 500-2000 lux, and see the light change color for 3-4 days, so that the white mycelium turns orange-yellow;

[0039] (4) In aseptic conditions, invert the PDA plate to an angle of 45°, pipette the sterile water to gently rinse the top of the mycelium 5 to 10 times, and collect the mixture;

[0040] (5) Dispense the collected mixture into 10ml EP tubes, set at 4°C, centrifugal force 3000-5000g, and centrifuge for...

Embodiment 3

[0047] (1) Under sterile conditions, use a pipette gun to draw 200 microliters of mature Cordyceps militaris liquid strain (the strain is derived from the spore powder variety of Changde Yandi Biotechnology Co., Ltd. strain bank), and inoculate it into the PDA medium On top, spread the mycelium evenly with a coating stick;

[0048] (2) Transfer the PDA plate coated with mycelium to a biochemical incubator, and incubate in the dark at 19-21°C for 4-5 days, so that the mycelium is covered with the entire PDA plate;

[0049] (3) Set the light intensity to 500-2000 lux, and see the light change color for 3-4 days, so that the white mycelium turns orange-yellow;

[0050](4) In aseptic conditions, invert the PDA plate to an angle of 45°, pipette the sterile water to gently rinse the top of the mycelium 5 to 10 times, and collect the mixture;

[0051] (5) Dispense the collected mixture into 10ml EP tubes, set at 4°C, centrifugal force 3000-5000g, and centrifuge for 10-15min to separ...

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Abstract

The invention relates to a method for selecting a high-cordycepin cordyceps militaris strain through ultraviolet-induced conidia. The method comprises the steps that firstly, conidia are separated andpurified; secondly, the high-activity conidia are selected through ultraviolet induction; thirdly, the high-cordycepin strain is cultured by using the totipotency of the conidia. In the induction way, most sterile spores can be killed by using ultraviolet rays, the induced mutation rate is high, the long sporocarp is high in ability, and it is beneficial for the sporocarp to enrich the cordycepinin the later period. The significance of conidia induced breeding in the method lies in entering a more microscopic conidia induced breeding stage from a mycelia induced breeding stage, artificial induction intervention with the strain is conducted in the front end of the life cycle, and the valuable strain can be more effectively selected.

Description

technical field [0001] The invention relates to the field of biological induction breeding, in particular to a method for screening cordycepin militaris strains through ultraviolet-induced conidia. Background technique [0002] Cordyceps militaris conidia are asexual spores produced during asexual reproduction. During the process of asexual reproduction, tiny hyphae grow from the mycelium, and the hyphae extend to form conidiophores, and clusters or clusters of totipotent spores are produced at the end of the conidiophores, which are conidia. [0003] Cordycepin (3'-deoxyadenosine) is a nucleoside substance with antibacterial activity. It has good effects in protecting the liver, protecting the kidney, and nourishing the lungs. It also has anti-cancer, anti-virus, and scavenging free radicals. pharmacological effects. Cordycepin can interfere with the synthesis of RNA and DNA in gene cells, inhibit the division of abnormal cells such as cancer cells, and can be used as a t...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N3/00C12R1/645
CPCC12N3/00C12N13/00
Inventor 阳武雄易思富王卫周丰杨子瑶
Owner CHANGDE YANDI BIOTECH LTD CO