A method for separating and preparing petunienin-3-o-glucoside

A technology of petunienin and glucoside, which is applied in the field of separation and preparation of petunienin-3-O-glucoside, and can solve the problems of undiscovered separation and preparation of petunin-3-O-glucoside monomers. Reporting and other problems, to achieve the effect of large sample processing volume and good repeatability

Active Publication Date: 2020-02-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, there is no research and report on the isolation and preparation of petunienin-3-O-glucoside monomers from blueberries

Method used

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  • A method for separating and preparing petunienin-3-o-glucoside
  • A method for separating and preparing petunienin-3-o-glucoside
  • A method for separating and preparing petunienin-3-o-glucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Wash 1kg of fresh blueberries, add 70% ethanol aqueous solution containing 0.1% (v / v) hydrochloric acid (the volume ratio of ethanol to water is 70 : 30) fully mixed, ultrasonically extracted for 90min, (control temperature below 45°C, protected from light), vacuum filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrate, and removed by vacuum rotary evaporation at 45°C ethanol to obtain the crude extract of anthocyanins.

[0067] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized water until neutral. The crude an...

Embodiment 2

[0072] Wash 5kg of fresh blueberries, add 70% ethanol solution containing 0.5% (v / v) hydrochloric acid according to the ratio of material to liquid (w / v) to fully mix, and extract by ultrasonic for 150min, (control the temperature at 45°C hereinafter, protected from light), vacuum suction filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrates, and removed ethanol by vacuum rotary evaporation at 45° C. to obtain crude anthocyanin extracts.

[0073] Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized water until neutral. ...

Embodiment 3

[0077] Wash 10kg of fresh blueberries, add 60% ethanol solution containing 0.1% (v / v) hydrochloric acid according to the ratio of solid to liquid (1:5 (w / v)) and mix thoroughly, ultrasonically extract for 200min, (control the temperature at 45°C hereinafter, protected from light), vacuum suction filtration after the end of ultrasonication, repeated extraction of the obtained filter residue once according to the above conditions, combined filtrates, and removed ethanol by vacuum rotary evaporation at 45° C. to obtain crude anthocyanin extracts.

[0078]Soak the AB-8 macroporous resin in ethanol for 24 hours, put it into the chromatography column, wash it with pure water until there is no alcohol smell, wash it with 0.5M sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash it with deionized water until the effluent is neutral; then use 0.5M hydrochloric acid solution to rinse for 1 hour at a flow rate of 2BV / h, and then rinse with deionized water until neut...

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Abstract

The invention discloses a method for preparing petunidin-3-O-glucoside by separation. The method includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, and a petunidin-3-O-glucoside monomer is prepared from blueberries with complex anthocyanin compositions by separation.Preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined for the first time, process parameters are optimized, a high-purity petunidin-3-O-glucoside monomer is prepared by separation from the blueberries, and the purity of the monomer can reach up to 99%.

Description

technical field [0001] The invention relates to the field of separation and purification of natural products, in particular to a method for the separation and preparation of petunienin-3-O-glucoside. Background technique [0002] Anthocyanin is a water-soluble pigment widely present in plants. It is a polyphenol compound formed by combining anthocyanin with one or more sugar groups, such as glucose, galactose, and arabinose, through glycosidic bonds. There are mainly 6 kinds of anthocyanins commonly found in nature, namely Pelargonidin, Cyaniain, Delphinidin, Peonidin, Petunidin and Malva pigment (Malvidin). In recent years, a large number of studies have confirmed that anthocyanins from natural sources have biological activities such as anti-oxidation, anti-tumor, prevention of cardiovascular diseases, alleviation of diabetes and obesity control. With the continuous improvement of people's living standards, people's calls for functional components from natural sources wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/065
CPCC07H1/08C07H17/065
Inventor 陈卫谢佳宏徐阳
Owner ZHEJIANG UNIV
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