Method for producing agricultural microbial agents from corn germ oil

Abstract

The invention discloses a method for producing agricultural microbial agents from corn germ oil. The method comprises the following steps: firstly, pretreating corn germ; secondly, performing wet steaming and softening continuously by water vapor, controlling rotation speed and temperature of a fry chamber, and filtering and purifying obtained oil; then, sterilizing the corn germ oil, and adding aculture medium to the corn germ oil; finally, transplanting a detection strain to the mixed culture medium until colonies appear on the mixed culture medium, and performing inactivation operation; performing constant temperature selective culture on the strains on the mixed culture medium until all the strains grow on the culture medium, crushing the culture medium, mixing the culture medium withorganic fertilizer for constant temperature and constant humidity fermentation to obtain the microbial agents. By direct preparation of the corn germ oil, cultivation and detection of subsequent strains are facilitated, gel solidification is performed on the basis of the culture medium, real-time observation is convenient, applicability is higher, selective cultivation can be performed, and massreproduction of the designated microbial agents is achieved in a short period of time.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a method for producing agricultural microbial agent by using corn germ oil. Background technique [0002] Bacteria can play a very important role in agricultural production. Among them, the most significant role is to improve soil quality, increase crop yield, and improve crop quality. Bacteria agent can reach above-mentioned beneficial effect, and its reason is: [0003] First of all, the nutrients are relatively comprehensive, and the organic fertilizer is used as the basis. At the same time, it is also equipped with corresponding microbial strains, which has the characteristics of multifunctional fertilizers; [0004] Secondly, the organic components and elements in the bacterial agent can be quickly dissolved in water, so that the nutrients in it can enter the soil with the water to achieve better results; [0005] Third, through the large number of bacteria...

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Problems solved by technology

Moreover, in the configuration process, it is necessary to detect the strains, and the national standard inspection method is still very traditional, mainly with the following defects: cumbersome operation, from the preparation of the medium to the actual inspection, the final sterilization and cleaning, is a micro-inspection Indispensable steps in traditional methods, these operations require high professional skills of technicians, and it takes a lot of time for preparation and aftercare work, which seriously affects work efficiency; low sensitivity

[0007] Therefore, the traditional method is not conducive to the rapid detection of the production line by the enterprise, and the most important thing is that the cost of the specific preparation process is high, it is difficult to effectively and quickly prepare the required products, and the quality and reliability of the prepared products are poor.

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