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Application of antibacterial peptide in preparing drugs for treating helicobacter pylori and drug composition

An anti-Helicobacter pylori, Helicobacter pylori technology, applied in the field of medicine, can solve the problems that antibiotics cannot cure Helicobacter pylori infection, the emergence of drug-resistant bacteria, high treatment costs, etc., to achieve the solution of antibiotic resistance and its side effects and good curative effect Effect

Active Publication Date: 2018-08-24
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the method of using antibiotics to treat Helicobacter pylori infection, the bacteria are easy to develop resistance to antibiotics through mutation, that is, the emergence of drug-resistant bacteria
With the increase of antibiotic-resistant strains, re-infection, complications, high treatment costs and poor treatment compliance, antibiotics cannot cure Helicobacter pylori infection

Method used

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  • Application of antibacterial peptide in preparing drugs for treating helicobacter pylori and drug composition
  • Application of antibacterial peptide in preparing drugs for treating helicobacter pylori and drug composition
  • Application of antibacterial peptide in preparing drugs for treating helicobacter pylori and drug composition

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0030] This experimental example provides an antibacterial peptide with anti-Helicobacter pylori activity, the amino acid sequence of which is shown in SEQ ID NO:1.

[0031] The screening of this antimicrobial peptide and the experimental process of anti-helicobacter pylori are as follows:

[0032] a. Screening of antimicrobial peptides: Antimicrobial peptides have their own unique antibacterial spectrum just like antibiotics. Not all antimicrobial peptides have antibacterial activity against Helicobacter pylori. The inventor screened out tens of thousands of antimicrobial peptides through experimental research. The target antibacterial peptide, the sequence is: GIGAS ILSAG KSALK GLAKG LAEHF AN, that is, the antibacterial peptide shown in SEQ ID NO: 1, denoted as BLP-1, and synthesized by chemical methods with a purity of 95%.

[0033] b. Helicobacter pylori culture and the determination of the number of bacteria: Prepare the culture medium, inoculate the test bacteria into th...

experiment example 2

[0040] This experimental example provides the anti-Helicobacter pylori activity test of the antibacterial peptide BLP-1 and the composition comprising the antibacterial peptide BLP-1:

[0041] A.1-Phenyl-naphthylamine (1-N-phenyl-naphthylamine, NPN) fluorescence test:

[0042] Test principle: 1-phenylnaphthylamine has weak fluorescence in aqueous solution, but when 1-phenylnaphthylamine penetrates into the hydrophobic environment of the bacterial cell wall outer membrane, its fluorescence increases. Therefore, according to the fluorescence intensity of 1-phenylnaphthylamine, the ability of each sample to penetrate the outer membrane of the Helicobacter pylori cell wall can be judged.

[0043] Test procedure: After centrifuging 50 mL of Helicobacter pylori in the logarithmic growth phase, the cells were collected and suspended in 5 mM N-2-hydroxyethylpiperazine-N'-2-sodium ethanesulfonate buffer (sodium N-2-hydroxyethylpiperazine -N'-2-ethanesulfonic acid, HEPES, pH7.2), prepa...

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Abstract

The invention provides application of antibacterial peptide in preparing drugs for treating helicobacter pylori and a drug composition, and relates to the field of drugs. According to research findings, the inventor discovers that antibacterial peptide with the amino acid sequence being shown as SEQ ID NO:1 has the strong killing activity on helicobacter pylori, and can serve as an active ingredient to prepare the drugs for treating helicobacter pylori diseases. The drug composition is prepared from the antibacterial peptide and honey, because honey can enhance the activity of the antibacterial peptide in killing helicobacter pylori, and thus the drug composition has a more prominent effect on resisting helicobacter pylori.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the use of an antimicrobial peptide in the preparation of medicines for treating Helicobacter pylori disease and a medicine composition. Background technique [0002] Helicobacter pylori was discovered by two Australian scientists in the 1980s and won the Nobel Prize in Physiology in 2005. At present, more than 50% of the world's population is infected with this pathogen, and 20% of those infected will cause clinical symptoms of chronic gastritis, peptic and duodenal ulcers, gastric cancer, and mucosa-associated lymphoid tissue tumors. The World Health Organization has listed it as a class I carcinogen. It is more popular in developing countries, and the infection rate of the population in some areas of our country is as high as 60%. [0003] To kill pathogenic microorganisms, the drug concentration must reach the minimum bactericidal concentration. Helicobacter pylori colonizes the hu...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K48/00A61K35/644A61P31/04A61P35/00A61P1/04
CPCA61K35/644A61K38/16A61K48/005A61P1/04A61P31/04A61P35/00A61K2300/00
Inventor 张孝林金光明汪建飞蒋安民张钦元
Owner ANHUI SCI & TECH UNIV
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