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A preparation method of graphene quantum dots-bovine serum albumin composite material applicable to electrochemical chiral recognition

A technology of bovine serum albumin and graphene quantum dots, applied in the fields of biotechnology and electrochemistry, can solve problems such as little progress, and achieve the effects of good identification ability, simple and environmentally friendly preparation method

Active Publication Date: 2019-12-31
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to the best of our knowledge, little progress has been made in electrochemical chiral recognition using biological structures such as amino acids, peptides and proteins

Method used

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  • A preparation method of graphene quantum dots-bovine serum albumin composite material applicable to electrochemical chiral recognition
  • A preparation method of graphene quantum dots-bovine serum albumin composite material applicable to electrochemical chiral recognition
  • A preparation method of graphene quantum dots-bovine serum albumin composite material applicable to electrochemical chiral recognition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of graphene quantum dots-bovine serum albumin composite includes the following steps:

[0036] (1) Add 2 g of citric acid monohydrate into a crucible, and heat the reaction at 200° C. for 30 min with a program-controlled box-type electric furnace. After the reaction, take out the solid sample, add 10mL ultrapure water, and dissolve it by ultrasonic. After the product was fully dissolved, a yellow transparent solution was obtained by suction filtration, and prepared into a 2 mg / mL graphene quantum dot solution.

[0037] (2) Add 5 mg of bovine serum albumin into 1 mL of 0.01M sodium dihydrogen phosphate solution with a pH of 7.4 to prepare a 5 mg / mL bovine serum albumin solution.

[0038] (3) Take 5 mL of the graphene quantum dot solution prepared in step (1) in a beaker, add N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt 80 mg each of salts, stirred at room temperature for 0.5 h, added 400 μL of the bovine serum albumin solu...

Embodiment 2

[0042] The graphene quantum dot-bovine serum albumin composite modified electrode prepared in Example 1 and a single bovine serum albumin modified electrode were placed in a 5mM potassium ferricyanide solution, and the electrochemical window of -0.2~0.6V The modified electrode was characterized by cyclic voltammetry, the scan rate was 0.1V / s, and the number of scan cycles was 20. The results are shown in the attached figure 2 As shown, the graphene quantum dots-bovine serum albumin composite modified electrode and a single bovine serum albumin modified electrode show reversible symmetric cyclic voltammograms.

Embodiment 3

[0044]Put the graphene quantum dot-bovine serum albumin composite modified electrode prepared in Example 1 in 25mL of 0.5mM tryptophan enantiomer solution, and after standing for 60s, the electrochemical window range of 0.4-1.2V The differential pulse voltammogram was recorded internally. After each measurement, the modified electrode was swept in 25 mL of 0.1 M sodium dihydrogen phosphate with pH=7 to restore the electrode activity. The graphene quantum dots-bovine serum albumin composite modified electrode for the recognition effect of tryptophan enantiomers is shown in the appendix image 3 , the graphene quantum dots-bovine serum albumin composite modified electrode has a certain recognition effect on tryptophan enantiomers (R L / D was 4.87).

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Abstract

The invention relates to a preparation method of a graphene quantum dot-bovine serum albumin composite material applied to electrochemical chiral recognition. The preparation method includes the following steps: preparation of a graphene quantum dot solution, preparation of a bovine serum albumin solution, preparation of a graphene quantum dot-bovine serum albumin composite material, preparation of a graphene quantum dot-bovine serum albumin composite material modified electrode, and electrochemical method identification of tryptophan enantiomers. The beneficial effects are that the preparation method of the graphene quantum dot-bovine serum albumin composite material modified electrode is simple and environment-friendly, and the graphene quantum dot-bovine serum albumin composite materialmodified electrode has better tryptophan enantiomer recognition ability because of certain chiral environment of the bovine serum albumin.

Description

technical field [0001] The invention relates to a preparation method of a graphene quantum dot-bovine serum albumin composite material applicable to electrochemical chiral recognition, belonging to the fields of biotechnology and electrochemistry. [0002] technical background [0003] Chirality and chiral analysis are of great significance in modern chemistry and chemical technology, so the development of simple and smart devices for enantioselective determination has become a research hotspot in life science and many other related fields. Currently, the most common methods for enantioselective detection, chromatography and electron mobility, suffer from high cost and time-consuming disadvantages. Amino acids are the molecular structural units of living organisms and play a vital role in many related fields such as living systems. Amino acids are important enantiomeric compounds, and their different structures play different or even opposite roles in life sciences. Therefo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 孔泳郭莉丽
Owner CHANGZHOU UNIV