A method for the determination of carbamate drug residues in poultry eggs
A carbamate and residue technology, which is applied in the field of physical and chemical detection of pesticide residues, can solve problems such as unused egg samples, and achieves the effects of good recovery rate and repeatability, high degree of automation, and simple processing and operation.
Inactive Publication Date: 2020-05-26
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] At present, there is no liquid chromatography tandem mass spectrometry method for the simultaneous determination of eight carbamate drugs in poultry eggs in China. The literature reports mainly include gas chromatography, gas-mass spectrometry, and liquid-mass spectrometry for the determination of one of the pesticides , the simultaneous determination of eight carbamate drugs in poultry eggs by high performance liquid chromatography tandem mass spectrometry has not been reported
In foreign literatures, gas chromatography-mass spectrometry (LC / MS-MS) has been used to detect carbamate drugs in vegetables, but it has not been used in poultry egg samples
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0034] Example 1 Using the method of the present invention, respectively determine carbosulfan, 3-hydroxy carbofuran, aldicarb sulfone, carbofuran, aldicarb, aldicarb sulfoxide in 6 poultry eggs according to the following steps , carbavir and methomyl residues:
[0035] (1) Sample preparation: poultry eggs were shelled, homogenized, frozen and stored to obtain poultry egg samples;
[0036](2) Sample pretreatment: Accurately weigh 2 g of poultry egg samples prepared in step (1), place them in a 50 mL polypropylene centrifuge tube, add 10 mL of 0.1% formic acid acetonitrile crude extract, homogenize for 1 min, and shake for 10 min , at 4°C, centrifuge at 8000 r / min for 8 min, extract the supernatant into a chicken heart bottle to obtain the extract; repeat the extraction once with 10 mL of 0.1% formic acid acetonitrile crude extract to obtain the extract; combine twice Add 2mL of isopropanol to the extract, condense to near dryness in a rotary evaporator at 50°C to obtain a con...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention relates to a method for determining carbamate drug residue in poultry eggs. The method comprises steps such as sample preparation; sample pretreatment; organic solvent extraction; use of a solid phase extraction column for removal of pigment impurities, blowing with nitrogen to near dry, dissolving of the residue in a mobile phase, and filtering membrane filtration of a solution for UPLC-TQS analysis; standard solution preparation; and high performance liquid chromatography-tandem mass spectrometry measurement. The method has the advantages of simple operation, rapidity,high degree of automation, fast extraction efficiency, good recovery rate and repeatability, and can simultaneously determine the residual amount of carbamate drugs in the poultry eggs.
Description
technical field [0001] The present invention relates to a method for the determination of carbamate drug residues in poultry eggs, in particular to a high performance liquid chromatography-tandem mass spectrometer method for the determination of eight carbamate drug residues in poultry eggs, which belongs to the field of pesticide residues Physical and chemical detection technology field. Background technique [0002] With the improvement of people's living standards, food safety and quality issues have attracted more and more attention from the people. Poultry and egg products are agricultural products consumed in people's daily life, and the problem of pesticide residues in them is directly related to the health of consumers. At the same time, after my country's accession to WTO, non-tariff technical barriers from egg importing countries have brought unprecedented pressure on my country's poultry and egg exports, among which the problem of excessive agricultural and veter...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 郭宏斌杨春亮叶剑芝林玲
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI