Procalcitonin chemiluminescence detection reagent based on nanometer antibodies and detection method thereof

Abstract

The invention relates to the technical field of biomedicine and biological detection, in particular to a reagent for testing procalcitonin in blood serum and a detection method thereof, in particularto a procalcitonin chemiluminescence detection reagent based on nanometer antibodies, a detection method thereof and application thereof to diagnosis. The detection reagent is prepared from a luminoussubstrate solution A, a luminous substrate solution B, a coating plate, enzyme conjugates, concentrated washing liquid, a calibration product and a quality control product, wherein the procalcitoninnanometer antibodies are covered on the coating plate; enzyme labelling procalcitonin nanometer antibodies are contained in the enzyme conjugates. Experiments show that the detection reagent and the detection method have the characteristics that the operation is simple and convenient; the specificity is high; the sensitivity is high; the detection speed is high; the detection result obtaining timeis short; the detection result is accurate and reliable, and the like. The detection accuracy in the experiment reaches 100 percent.

Description

technical field [0001] The invention relates to the technical field of biomedicine and biological detection, in particular to a reagent for measuring procalcitonin in serum and a detection method thereof, in particular to a nanobody-based procalcitonin chemiluminescence detection reagent, detection method and its application in diagnosis. Background technique [0002] Procalcitonin (PCT for short) is a protein in human blood. Its level in plasma increases in severe bacterial, fungal, and parasitic infections, as well as sepsis and multiple organ failure. Autoimmunity, allergies, and viral infections do not increase PCT, and local limited bacterial infections, minor infections, and chronic inflammation do not cause it to increase. PCT reflects the active degree of systemic inflammatory response. Factors affecting PCT level include the size and type of infected organ, the type of bacteria, the degree of inflammation and the status of immune response. [0003] The detection o...

AI Technical Summary

Problems solved by technology

However, the above methods all need to be coated and labeled with a pair of monoclonal antibodies against PCT, and the monoclonal antibodies will decompose under high temperature and strong acid and strong alkali conditions, and need to be stored below zero, otherwise they will lose their activity within a few weeks. Poor stability. In addition, traditional monoclonal antibodies have problems such as low solubility and difficult preparation. At the same time, the macromolecular properties of traditional monoclonal antibodies also seriously restrict their wide application.

[0004] Not only that, in addition to the detection stability and sensitivity need to be improved, the existing chemiluminescence detection method using monoclonal antibody detection reagents also has defects such as complicated operation process, long time-consuming, and slow detection results.

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